FastQCFastQC Report
Thu 2 Feb 2017
SRR4062296_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062296_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197297
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12630.10548761084342481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3300.014.9629091
GGTCTAC851.7254933E-814.5228241
GCGTTAT400.00529406214.243541
CGGTCCA1153.765308E-1013.2196910
ACCGTCG957.376366E-813.000638
ATCTAAT604.1064766E-412.6609251
GTATCAA9450.012.560441
GTTACAC701.0921316E-412.2123233
CGGACCA1103.8009603E-812.0930139
GTACTAG550.003078170112.0854271
TAACGCC951.0395361E-611.9995784
CGCATCG802.8611905E-511.8765713
TTAACCA1251.830449E-811.3995984
CCTAATA1001.9328654E-611.3976912
TATACTG1604.5474735E-1111.2813255
GATTAAG2450.011.23979951
ATACCGT1104.9608025E-711.2278186
TAATACC855.322153E-511.1760774
AGCGAAA1201.2745113E-711.08294417
GCGAAAG1201.2759847E-711.08201618
Produced by FastQC (version 0.11.5)