##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062296_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1197297
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260558574856532	32.0	32.0	32.0	32.0	32.0
2	30.79997694807554	32.0	32.0	32.0	32.0	32.0
3	30.821139616987264	32.0	32.0	32.0	32.0	32.0
4	30.83526894329477	32.0	32.0	32.0	32.0	32.0
5	30.747145445115123	32.0	32.0	32.0	32.0	32.0
6	34.399862356624965	36.0	36.0	36.0	32.0	36.0
7	34.33958658545039	36.0	36.0	36.0	32.0	36.0
8	34.29789935162287	36.0	36.0	36.0	32.0	36.0
9	34.438270537719546	36.0	36.0	36.0	32.0	36.0
10	34.1283908670948	36.0	36.0	36.0	32.0	36.0
11	34.414192134449515	36.0	36.0	36.0	32.0	36.0
12	34.24273175327425	36.0	36.0	36.0	32.0	36.0
13	34.320265564851496	36.0	36.0	36.0	32.0	36.0
14	34.2276043454548	36.0	36.0	36.0	32.0	36.0
15	34.18824652529823	36.0	36.0	36.0	32.0	36.0
16	34.18281679483036	36.0	36.0	36.0	32.0	36.0
17	34.13615585773622	36.0	36.0	36.0	32.0	36.0
18	34.129628655212535	36.0	36.0	36.0	32.0	36.0
19	34.13466499957822	36.0	36.0	36.0	32.0	36.0
20	34.13065931009599	36.0	36.0	36.0	32.0	36.0
21	34.11397005087292	36.0	36.0	36.0	32.0	36.0
22	34.0871037010867	36.0	36.0	36.0	32.0	36.0
23	34.056293467702666	36.0	36.0	36.0	32.0	36.0
24	34.01433562432713	36.0	36.0	36.0	32.0	36.0
25	33.6677307301363	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	16.0
5	91.0
6	250.0
7	50.0
8	185.0
9	168.0
10	91.0
11	22.0
12	60.0
13	58.0
14	131.0
15	200.0
16	322.0
17	481.0
18	631.0
19	898.0
20	1360.0
21	1951.0
22	3017.0
23	4493.0
24	6689.0
25	9515.0
26	13459.0
27	17620.0
28	23860.0
29	32071.0
30	42466.0
31	58044.0
32	81908.0
33	115728.0
34	250305.0
35	531156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72070233055006	17.512075378089005	11.56889292399131	26.198329367369627
2	16.661488322834657	19.05964616877726	37.76482635008611	26.514039158301973
3	19.578982183917166	22.33880040658521	28.051289204193807	30.030928205303812
4	13.242090755319817	15.329588406039218	34.912272641844346	36.516048196796625
5	14.990588115375585	36.08599279299452	33.77600800741254	15.14741108421736
6	35.324489076166806	34.40384837174629	16.327666523478175	13.943996028608726
7	30.71219135350887	29.893760309814905	19.996254396694503	19.39779393998172
8	27.629584355144704	34.13202541477947	18.75606711388072	19.482323116195108
9	26.31163922499937	15.330010787125692	18.78013496450283	39.5782150233721
10	16.449181367100635	27.228440269529564	31.343140034439703	24.979238328930105
11	36.61779342762272	21.799667115530987	21.717115112787614	19.86542434405868
12	23.86711951499175	23.785827609307088	28.97780446620406	23.369248409497104
13	29.508095269953838	19.805730246872283	24.73615106710377	25.950023416070117
14	22.943472083900904	19.628108675819707	25.23440759871125	32.194011641568146
15	24.854856110595378	27.169715064689598	22.11748668155322	25.857942143161804
16	25.423903145124267	26.41599998662041	23.429423899800224	24.7306729684551
17	23.985663226309285	26.418164819377054	25.17582283413865	24.42034912017501
18	24.38623233910261	25.948945300447434	25.596249957146426	24.068572403303527
19	25.246881583787577	25.73061231314584	25.108851954653378	23.9136541484132
20	25.19681287290365	25.14372752755213	25.114969482196347	24.544490117347873
21	25.37506081728398	25.047943247233334	25.059730514643796	24.517265420838886
22	25.30984445908481	24.850512171385954	25.082435681774857	24.75720768775437
23	24.324683531606716	25.08057990785136	25.35934960980601	25.235386950735915
24	24.478123006727074	25.369604526291766	25.329394228944558	24.8228782380366
25	24.764763710413597	24.99072095659414	25.192017682011365	25.052497650980904
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	79.0
1	79.0
2	178.0
3	277.0
4	277.0
5	277.0
6	633.5
7	990.0
8	990.0
9	990.0
10	1413.5
11	1837.0
12	1837.0
13	1837.0
14	2767.5
15	3698.0
16	3698.0
17	3698.0
18	6053.5
19	8409.0
20	8409.0
21	8409.0
22	12788.0
23	17167.0
24	17167.0
25	17167.0
26	24630.0
27	32093.0
28	32093.0
29	32093.0
30	40862.5
31	49632.0
32	49632.0
33	49632.0
34	61891.0
35	74150.0
36	74150.0
37	74150.0
38	87495.0
39	100840.0
40	100840.0
41	100840.0
42	114185.5
43	127531.0
44	127531.0
45	127531.0
46	139878.5
47	152226.0
48	152226.0
49	152226.0
50	157057.0
51	161888.0
52	161888.0
53	161888.0
54	155527.5
55	149167.0
56	149167.0
57	149167.0
58	136050.5
59	122934.0
60	122934.0
61	122934.0
62	106777.5
63	90621.0
64	90621.0
65	90621.0
66	72175.0
67	53729.0
68	53729.0
69	53729.0
70	40511.5
71	27294.0
72	27294.0
73	27294.0
74	19864.5
75	12435.0
76	12435.0
77	12435.0
78	9250.5
79	6066.0
80	6066.0
81	6066.0
82	4212.0
83	2358.0
84	2358.0
85	2358.0
86	1668.0
87	978.0
88	978.0
89	978.0
90	648.0
91	318.0
92	318.0
93	318.0
94	211.5
95	105.0
96	105.0
97	105.0
98	290.0
99	475.0
100	475.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0021715581012898217
2	2.5056439630267177E-4
3	6.681717234737914E-4
4	0.01018961878297532
5	0.03390971496629491
6	0.06172236295589149
7	0.10256435955322697
8	0.10857790506449111
9	0.11918513117463753
10	0.1322980012478107
11	0.14090071218753575
12	0.13413547348736363
13	0.12837249237240217
14	0.11776526626225572
15	0.13254856564411335
16	0.1207720390178878
17	0.12453050496242786
18	0.11392327885228143
19	0.09830476481608155
20	0.09270882663198855
21	0.0911219187887383
22	0.10131153757171361
23	0.08093230000576299
24	0.09070431146156718
25	0.08794810310223779
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1197297.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.05820751595717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.95284728419003	39.77489436905143
2	16.182989730245286	18.14378793053388
3	5.974476517761521	10.047553332956653
4	2.629803571954701	5.896882974513681
5	1.3799445936715686	3.867861019628199
6	0.8013466724966967	2.695323483542495
7	0.4910192436809703	1.9267961059617322
8	0.3211553943307228	1.440271659220857
9	0.22345759374994534	1.1273968945305801
>10	0.95201694722847	9.182506688895458
>50	0.05681536092823854	2.1974766917380286
>100	0.03337869109884121	3.3835038916550286
>500	5.987189304608472E-4	0.20976943779691315
>1k	1.496797326152118E-4	0.1059755199750005
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1263	0.10548761084342481	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.340858617368957E-4
2	0.0	0.0	0.0	0.0	3.340858617368957E-4
3	0.0	0.0	0.0	0.0	3.340858617368957E-4
4	0.0	0.0	0.0	0.0	3.340858617368957E-4
5	0.0	2.5056439630267177E-4	0.0	0.0	4.1760732717111964E-4
6	0.0	2.5056439630267177E-4	0.0	0.0	5.011287926053435E-4
7	0.0	3.340858617368957E-4	0.0	0.0	5.011287926053435E-4
8	0.0	3.340858617368957E-4	0.0	8.352146543422392E-5	5.011287926053435E-4
9	0.0	3.340858617368957E-4	0.0	8.352146543422392E-5	5.011287926053435E-4
10	0.0	3.340858617368957E-4	0.0	8.352146543422392E-5	5.011287926053435E-4
11	0.0	3.340858617368957E-4	0.0	8.352146543422392E-5	5.011287926053435E-4
12	0.0	3.340858617368957E-4	0.0	8.352146543422392E-5	0.001002257585210687
13	0.0	3.340858617368957E-4	0.0	8.352146543422392E-5	0.001169300516079135
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	330	0.0	14.962909	1
GGTCTAC	85	1.7254933E-8	14.522824	1
GCGTTAT	40	0.005294062	14.24354	1
CGGTCCA	115	3.765308E-10	13.21969	10
ACCGTCG	95	7.376366E-8	13.00063	8
ATCTAAT	60	4.1064766E-4	12.660925	1
GTATCAA	945	0.0	12.56044	1
GTTACAC	70	1.0921316E-4	12.212323	3
CGGACCA	110	3.8009603E-8	12.093013	9
GTACTAG	55	0.0030781701	12.085427	1
TAACGCC	95	1.0395361E-6	11.999578	4
CGCATCG	80	2.8611905E-5	11.87657	13
TTAACCA	125	1.830449E-8	11.399598	4
CCTAATA	100	1.9328654E-6	11.397691	2
TATACTG	160	4.5474735E-11	11.281325	5
GATTAAG	245	0.0	11.2397995	1
ATACCGT	110	4.9608025E-7	11.227818	6
TAATACC	85	5.322153E-5	11.176077	4
AGCGAAA	120	1.2745113E-7	11.082944	17
GCGAAAG	120	1.2759847E-7	11.082016	18
>>END_MODULE