FastQCFastQC Report
Thu 2 Feb 2017
SRR4062296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197297
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9400.023.643641
GGTATCA4500.021.5159721
GGCGTTA1850.020.21909542
TAGTGCG550.00448422819.99950620
CGGTCCA1252.2884706E-819.35952410
CGCAATA1456.3046173E-918.20720736
TCAACGC12600.017.8090844
GTATAGA2350.017.7912541
ATCAACG12700.017.6688543
CAACGCA12800.017.3589465
GCGTTAT2055.456968E-1217.17245943
CGAGCCG2055.456968E-1217.17030715
CAATACG1451.2483542E-716.69063838
GTATTAG2007.2759576E-1116.5037291
CGTTATT2400.016.5016644
GTAATAC1602.1296728E-816.4995923
CGGCGTT2151.2732926E-1116.37305641
TATCAAC14000.016.3424532
AACGCAG13600.016.3378336
GTATTAC1351.0828662E-616.2999781