##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062296_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1197297
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229733307608722	32.0	32.0	32.0	32.0	32.0
2	31.384834339349386	32.0	32.0	32.0	32.0	32.0
3	31.53419243512679	32.0	32.0	32.0	32.0	32.0
4	31.621386339396157	32.0	32.0	32.0	32.0	32.0
5	31.597685453149886	32.0	32.0	32.0	32.0	32.0
6	35.22470949146285	36.0	36.0	36.0	36.0	36.0
7	35.23604335432228	36.0	36.0	36.0	36.0	36.0
8	35.18343318324526	36.0	36.0	36.0	36.0	36.0
9	35.27875289088672	36.0	36.0	36.0	36.0	36.0
10	35.14589696624981	36.0	36.0	36.0	36.0	36.0
11	35.275475508583085	36.0	36.0	36.0	36.0	36.0
12	35.19466765556082	36.0	36.0	36.0	36.0	36.0
13	35.23182134424458	36.0	36.0	36.0	36.0	36.0
14	35.19921205849509	36.0	36.0	36.0	36.0	36.0
15	35.17497078836747	36.0	36.0	36.0	36.0	36.0
16	35.19210605221595	36.0	36.0	36.0	36.0	36.0
17	35.17110959102044	36.0	36.0	36.0	36.0	36.0
18	35.16626785166922	36.0	36.0	36.0	36.0	36.0
19	35.15994026544792	36.0	36.0	36.0	36.0	36.0
20	35.165549567066485	36.0	36.0	36.0	36.0	36.0
21	35.14939985651012	36.0	36.0	36.0	36.0	36.0
22	35.13683655767951	36.0	36.0	36.0	36.0	36.0
23	35.11321251118144	36.0	36.0	36.0	36.0	36.0
24	35.097173884174104	36.0	36.0	36.0	36.0	36.0
25	35.08139751456823	36.0	36.0	36.0	36.0	36.0
26	35.038835810997604	36.0	36.0	36.0	36.0	36.0
27	35.021091675666106	36.0	36.0	36.0	36.0	36.0
28	35.01140986739297	36.0	36.0	36.0	36.0	36.0
29	34.98608782950262	36.0	36.0	36.0	36.0	36.0
30	34.96374834314293	36.0	36.0	36.0	36.0	36.0
31	34.97111243075027	36.0	36.0	36.0	36.0	36.0
32	34.94429034734072	36.0	36.0	36.0	36.0	36.0
33	34.93744325760442	36.0	36.0	36.0	36.0	36.0
34	34.918195735895104	36.0	36.0	36.0	36.0	36.0
35	34.911614244418885	36.0	36.0	36.0	36.0	36.0
36	34.894965910713886	36.0	36.0	36.0	32.0	36.0
37	34.88458419256041	36.0	36.0	36.0	32.0	36.0
38	34.87269491195585	36.0	36.0	36.0	32.0	36.0
39	34.85395436554172	36.0	36.0	36.0	32.0	36.0
40	34.852065109993596	36.0	36.0	36.0	32.0	36.0
41	34.81921027113573	36.0	36.0	36.0	32.0	36.0
42	34.8074997264672	36.0	36.0	36.0	32.0	36.0
43	34.786838186348085	36.0	36.0	36.0	32.0	36.0
44	34.75109266957154	36.0	36.0	36.0	32.0	36.0
45	34.74138246400016	36.0	36.0	36.0	32.0	36.0
46	34.72367841897207	36.0	36.0	36.0	32.0	36.0
47	34.70321983601396	36.0	36.0	36.0	32.0	36.0
48	34.68994911037111	36.0	36.0	36.0	32.0	36.0
49	34.67864865609786	36.0	36.0	36.0	32.0	36.0
50	34.25283367451852	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	21.0
22	79.0
23	197.0
24	524.0
25	1268.0
26	2596.0
27	5115.0
28	9034.0
29	14434.0
30	22298.0
31	33099.0
32	51206.0
33	88194.0
34	201114.0
35	768106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.325062242050564	18.0102594950457	12.059668822163182	26.605009440740556
2	16.096753419771822	19.610116360362138	37.42951432015095	26.863615899715093
3	18.99163945243007	22.779276532836658	28.343676135272155	29.885407879461116
4	13.026352714783979	15.80427897529099	34.91350509815451	36.255863211770524
5	14.710134578137254	36.368586908678466	33.6736833049778	15.247595208206485
6	35.45870406423803	34.564857341160966	16.164410334277964	13.812028260323045
7	30.761874455544447	29.878718480042966	20.04982890627806	19.30957815813453
8	27.73321907596862	34.21197914970137	18.719164918980002	19.335636855350007
9	26.47822553635397	15.050735114178018	18.607329676763577	39.86370967270443
10	16.292893564242732	27.223700090620937	31.621780764139164	24.861625580997167
11	36.9502303939624	21.570671270369843	21.64517241753717	19.83392591813059
12	23.783779269102435	23.792966645647063	28.95760860940704	23.465645475843463
13	29.651373051131007	19.750237409765496	24.735299595672586	25.86308994343091
14	22.971910895959816	19.438201214903238	25.281195893750674	32.308691995386276
15	24.910694673084453	27.171954828250634	22.066120603325658	25.85122989533925
16	25.54094945848677	26.23008737209689	23.470612456600012	24.75835071281633
17	24.137703510490713	26.35645123975087	25.14472181923115	24.36112343052726
18	24.597092114230733	25.768147559166653	25.570285042295303	24.064475284307303
19	25.384428425027373	25.6185390926395	25.05978048888455	23.937251993448577
20	25.37914664234516	25.07997200353464	25.122317492612506	24.4185638615077
21	25.568927342171577	24.983107783615928	25.00908295936597	24.438881914846526
22	25.42385055671233	24.906685642743614	25.06512586267234	24.604337937871723
23	24.495696970506874	24.908878005104835	25.304603038847535	25.290821985540752
24	24.58719136446931	25.196402530389566	25.36837480769006	24.84803129745107
25	24.87492587426605	24.796916369467713	25.336217624802682	24.991940131463554
26	24.616217263624915	25.43874009013674	25.414852851267693	24.53018979497065
27	24.973690540621803	25.130294465492014	25.016537374466292	24.87947761941989
28	24.78492917279166	25.155268608846722	25.279132700788452	24.780669517573166
29	24.673972053554277	25.573336451486274	25.203334196393524	24.549357298565926
30	24.8288620873981	25.302769611118535	25.233195242549776	24.63517305893358
31	24.83531572524975	25.095653437635463	25.144597261651512	24.924433575463276
32	24.774780794435376	25.205340781339004	24.92462068124529	25.095257742980326
33	24.562284148852203	25.046375371149132	25.344046371195066	25.047294108803598
34	25.045352496642415	25.093628266549068	25.246139608849333	24.614879627959187
35	25.014115186604435	25.00576300518169	25.405665451702674	24.57445635651119
36	24.639833357415437	25.237682004321414	25.293641573115355	24.828843065147797
37	25.07598517631317	25.086258522492365	25.231755831794416	24.606000469400044
38	24.55029954973578	25.15382565896348	25.42618915774449	24.86968563355625
39	24.944353265930836	25.17069870582192	24.981938302075115	24.903009726172133
40	25.121836937702174	25.057107801990654	25.20819813296116	24.612857127346015
41	24.53018665579762	25.369252407949993	25.470815612670012	24.629745323582377
42	25.24078357083852	25.241952929954554	24.996387515587976	24.52087598361895
43	24.82115249208811	24.992127786283387	25.205533685975094	24.9811860356534
44	24.561431350423096	25.064752720198925	25.275736752733565	25.09807917664442
45	24.50733752620545	25.452988883042256	25.210353554336113	24.82932003641618
46	24.59371985316534	25.274855608407492	25.10363202799714	25.027792510430018
47	24.653527450949277	25.318367200348124	25.414668736913054	24.613436611789545
48	24.75510422269813	25.5970378636268	24.977114105395394	24.67074380827968
49	24.75005220296513	25.29455001044059	25.03587387763625	24.91952390895803
50	24.80297006548176	25.482927970065482	25.1513430442336	24.562758920219164
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	42.0
2	37.0
3	86.5
4	136.0
5	135.5
6	135.0
7	112.0
8	89.0
9	131.5
10	174.0
11	264.5
12	355.0
13	662.0
14	969.0
15	1235.0
16	1501.0
17	1646.0
18	1791.0
19	1927.0
20	2063.0
21	2682.5
22	3302.0
23	4235.5
24	5169.0
25	6593.5
26	8018.0
27	10016.5
28	12015.0
29	14222.5
30	16430.0
31	18971.5
32	21513.0
33	24129.0
34	26745.0
35	31015.5
36	35286.0
37	39233.0
38	43180.0
39	47714.0
40	52248.0
41	56999.0
42	61750.0
43	65283.0
44	68816.0
45	73893.0
46	78970.0
47	83069.0
48	87168.0
49	89758.0
50	92348.0
51	92477.0
52	92606.0
53	91883.5
54	91161.0
55	89487.5
56	87814.0
57	83301.0
58	78788.0
59	72931.5
60	67075.0
61	59080.0
62	51085.0
63	44038.5
64	36992.0
65	31117.0
66	25242.0
67	21150.0
68	17058.0
69	14586.5
70	12115.0
71	9362.5
72	6610.0
73	5537.5
74	4465.0
75	3368.5
76	2272.0
77	1892.0
78	1512.0
79	1285.5
80	1059.0
81	817.5
82	576.0
83	448.5
84	321.0
85	241.5
86	162.0
87	115.5
88	69.0
89	45.5
90	22.0
91	19.0
92	16.0
93	11.0
94	6.0
95	5.5
96	5.0
97	6.0
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02981716316001794
2	0.005679459649527227
3	5.846502580395675E-4
4	8.352146543422392E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.6704293086844784E-4
11	0.0
12	1.6704293086844784E-4
13	0.0
14	0.0
15	0.0
16	3.340858617368957E-4
17	0.0
18	8.352146543422392E-5
19	0.0
20	2.5056439630267177E-4
21	0.0
22	0.0
23	8.352146543422392E-5
24	9.187361197764632E-4
25	5.846502580395675E-4
26	4.176073271711196E-4
27	7.516931889080153E-4
28	0.0014198649123818067
29	5.846502580395675E-4
30	0.0014198649123818067
31	5.011287926053435E-4
32	0.0015869078432502544
33	1.6704293086844784E-4
34	7.516931889080153E-4
35	4.176073271711196E-4
36	3.340858617368957E-4
37	0.002004515170421374
38	0.0
39	0.001002257585210687
40	0.0
41	0.0010857790506449108
42	0.005011287926053435
43	0.004009030340842748
44	0.004176073271711196
45	0.0022550795667240463
46	0.0026726868938951654
47	0.0013363434469475827
48	0.004426637668013868
49	0.003925508875408524
50	0.0014198649123818067
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1197297.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.78746774398073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.64948563435713	40.12005386589885
2	16.35631330315423	18.576672282266273
3	6.114335960626937	10.416529684198906
4	2.696601802389575	6.125327514862331
5	1.377287258804633	3.910632789178186
6	0.7903775714595808	2.6930124506896056
7	0.4781328765558084	1.9006368713324777
8	0.32421763630861994	1.4729198851124343
9	0.23348826869178663	1.1933286774239464
>10	0.9045004448033002	8.755961709982348
>50	0.04802959687863784	1.8761127713208683
>100	0.0263417262027919	2.68291520811749
>500	8.879197670284251E-4	0.2758962896162005
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.352146543422392E-5
2	0.0	0.0	0.0	0.0	8.352146543422392E-5
3	0.0	0.0	0.0	0.0	8.352146543422392E-5
4	0.0	0.0	0.0	0.0	8.352146543422392E-5
5	0.0	8.352146543422392E-5	0.0	0.0	8.352146543422392E-5
6	0.0	8.352146543422392E-5	0.0	0.0	8.352146543422392E-5
7	0.0	8.352146543422392E-5	0.0	0.0	8.352146543422392E-5
8	0.0	8.352146543422392E-5	0.0	0.0	8.352146543422392E-5
9	0.0	8.352146543422392E-5	0.0	0.0	8.352146543422392E-5
10	0.0	8.352146543422392E-5	0.0	0.0	2.5056439630267177E-4
11	0.0	8.352146543422392E-5	0.0	0.0	2.5056439630267177E-4
12	0.0	8.352146543422392E-5	0.0	0.0	5.846502580395675E-4
13	0.0	8.352146543422392E-5	0.0	0.0	7.516931889080153E-4
14	0.0	8.352146543422392E-5	0.0	0.0	7.516931889080153E-4
15	0.0	8.352146543422392E-5	0.0	1.6704293086844784E-4	0.001002257585210687
16	0.0	8.352146543422392E-5	0.0	1.6704293086844784E-4	0.001085779050644911
17	0.0	8.352146543422392E-5	0.0	2.5056439630267177E-4	0.001169300516079135
18	0.0	8.352146543422392E-5	0.0	2.5056439630267177E-4	0.0014198649123818067
19	0.0	8.352146543422392E-5	0.0	5.846502580395675E-4	0.0014198649123818067
20	0.0	8.352146543422392E-5	0.0	0.001002257585210687	0.0015869078432502546
21	0.0	8.352146543422392E-5	0.0	0.0014198649123818067	0.0015869078432502546
22	0.0	8.352146543422392E-5	0.0	0.0018374722395529263	0.0015869078432502546
23	0.0	8.352146543422392E-5	0.0	0.002255079566724046	0.0015869078432502546
24	0.0	8.352146543422392E-5	0.0	0.0028397298247636133	0.0015869078432502546
25	0.0	8.352146543422392E-5	0.0	0.0035079015482374046	0.0015869078432502546
26	0.0	8.352146543422392E-5	0.0	0.004927766460619211	0.0015869078432502546
27	0.0	8.352146543422392E-5	0.0	0.005261852322356107	0.0015869078432502546
28	0.0	8.352146543422392E-5	0.0	0.006681717234737914	0.0015869078432502546
29	0.0	8.352146543422392E-5	0.0	0.00893679680146196	0.0015869078432502546
30	0.0	1.6704293086844784E-4	0.0	0.012444698349699364	0.0015869078432502546
31	0.0	1.6704293086844784E-4	0.0	0.020629801962253308	0.0017539507741187023
32	0.0	1.6704293086844784E-4	0.0	0.02831377678220191	0.0017539507741187023
33	0.0	1.6704293086844784E-4	0.0	0.037668180910834986	0.002004515170421374
34	0.0	1.6704293086844784E-4	0.0	0.04718962797033652	0.00233860103215827
35	0.0	1.6704293086844784E-4	0.0	0.058465025803956745	0.00233860103215827
36	0.0	1.6704293086844784E-4	0.0	0.07491875449449886	0.00233860103215827
37	0.0	2.5056439630267177E-4	0.0	0.09588264231848906	0.00233860103215827
38	0.0	2.5056439630267177E-4	0.0	0.12369529030808563	0.0024221224975924938
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	940	0.0	23.64364	1
GGTATCA	450	0.0	21.515972	1
GGCGTTA	185	0.0	20.219095	42
TAGTGCG	55	0.004484228	19.999506	20
CGGTCCA	125	2.2884706E-8	19.359524	10
CGCAATA	145	6.3046173E-9	18.207207	36
TCAACGC	1260	0.0	17.809084	4
GTATAGA	235	0.0	17.791254	1
ATCAACG	1270	0.0	17.668854	3
CAACGCA	1280	0.0	17.358946	5
GCGTTAT	205	5.456968E-12	17.172459	43
CGAGCCG	205	5.456968E-12	17.170307	15
CAATACG	145	1.2483542E-7	16.690638	38
GTATTAG	200	7.2759576E-11	16.503729	1
CGTTATT	240	0.0	16.50166	44
GTAATAC	160	2.1296728E-8	16.499592	3
CGGCGTT	215	1.2732926E-11	16.373056	41
TATCAAC	1400	0.0	16.342453	2
AACGCAG	1360	0.0	16.337833	6
GTATTAC	135	1.0828662E-6	16.299978	1
>>END_MODULE