FastQCFastQC Report
Thu 2 Feb 2017
SRR4062295_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062295_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566262
Sequences flagged as poor quality0
Sequence length25
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13220.23346083615005067No Hit
TATCAACGCAGAGTACTTTTTTTTT8460.14940080740010805No Hit
GGGTAGGCACACGCTGAGCCAGTCA8430.14887101730294455No Hit
GTGCATGGCCGTTCTTAGTTGGTGG8420.14869442060389007No Hit
TCGTAGTTCCGACCATAAACGATGC8400.14834122720578108No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC8380.1479880338076721No Hit
ATCAGATACCGTCGTAGTTCCGACC7970.14074756914643752No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7670.13544966817480247No Hit
GTACATGGGGAATAATTGCAATCCC6820.12043894875516986No Hit
GTACATGGGGTGGTATCAACGCAAA6770.11955596525989737No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6590.11637722467691634No Hit
GTATCAACGCAGAGTACATGGGGAA6530.11531764448258933No Hit
ACCCCCGAGCGGTCGGCGTCCCCCA6410.11319848409393532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA402.75683E-416.6253094
AATTTCA1101.2732926E-1114.68079419
GATAACG653.3592332E-614.61694911
ATAACGA653.3592332E-614.61694912
TAACGAA653.365405E-614.61436513
GCCGCGC400.005266773514.2527878
ACCGTCC400.005266773514.2527878
CTACACT602.5621153E-514.2502654
CCGATAA707.228413E-613.5740819
CGATAAC707.228413E-613.57408110
CAAGACG1051.3624231E-913.5716814
AACGAAC707.2548755E-613.56928214
GACCGTC500.001495286213.30267
GTCCTAA500.001501276913.2955461
AAGAATT1251.0186341E-1012.91909816
AAGACGG1051.9826984E-812.6669035
CGAACGA751.47570845E-512.66578316
CCAGAAC701.089246E-412.2134333
GAATTTC1405.2750693E-1112.21343318
ATAACGC550.003065900912.0900653
Produced by FastQC (version 0.11.5)