##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062295_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	566262
Sequences flagged as poor quality	0
Sequence length	25
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23471997061431	32.0	32.0	32.0	32.0	32.0
2	30.96817550886339	32.0	32.0	32.0	32.0	32.0
3	31.011936170889093	32.0	32.0	32.0	32.0	32.0
4	30.968934874669323	32.0	32.0	32.0	32.0	32.0
5	31.000309044223346	32.0	32.0	32.0	32.0	32.0
6	34.61026521292264	36.0	36.0	36.0	32.0	36.0
7	34.543621150633456	36.0	36.0	36.0	32.0	36.0
8	34.500429129978706	36.0	36.0	36.0	32.0	36.0
9	34.55161568319965	36.0	36.0	36.0	32.0	36.0
10	34.39637482296181	36.0	36.0	36.0	32.0	36.0
11	34.56603833561143	36.0	36.0	36.0	32.0	36.0
12	34.45822428487167	36.0	36.0	36.0	32.0	36.0
13	34.51694621924127	36.0	36.0	36.0	32.0	36.0
14	34.462174753029515	36.0	36.0	36.0	32.0	36.0
15	34.44713931007202	36.0	36.0	36.0	32.0	36.0
16	34.442463029481054	36.0	36.0	36.0	32.0	36.0
17	34.380126160681804	36.0	36.0	36.0	32.0	36.0
18	34.40985444900064	36.0	36.0	36.0	32.0	36.0
19	34.395041517883946	36.0	36.0	36.0	32.0	36.0
20	34.33007865616976	36.0	36.0	36.0	32.0	36.0
21	34.30119803200638	36.0	36.0	36.0	32.0	36.0
22	34.29120795674087	36.0	36.0	36.0	32.0	36.0
23	34.31064418943881	36.0	36.0	36.0	32.0	36.0
24	34.296299239574616	36.0	36.0	36.0	32.0	36.0
25	33.99017769159859	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	6.0
5	54.0
6	120.0
7	26.0
8	101.0
9	88.0
10	42.0
11	15.0
12	22.0
13	26.0
14	89.0
15	127.0
16	213.0
17	275.0
18	379.0
19	603.0
20	911.0
21	1236.0
22	1843.0
23	2458.0
24	3199.0
25	4379.0
26	5849.0
27	7122.0
28	9289.0
29	11926.0
30	15612.0
31	20840.0
32	29941.0
33	43558.0
34	105492.0
35	300421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.39129513402018	14.819393280094939	10.671813908147875	27.117497677737003
2	18.695619157950134	17.814929864497113	34.05461439159681	29.43483658595595
3	21.534454137675276	20.510719457493025	25.210150814113657	32.74467559071804
4	15.080546739362727	14.263157615865968	29.816687949162212	40.83960769560909
5	17.533176460612225	34.14030922298539	29.645764436530147	18.68074987987224
6	38.225714957243056	30.04843843053123	15.504008850042325	16.221837762183387
7	33.872869773995816	27.883042879444986	17.21454220141476	21.02954514514444
8	31.052333804809052	29.38719943422914	17.22807637906648	22.33239038189533
9	28.643017042299846	13.798639869574597	16.820916160655237	40.737426927470324
10	19.589291588117327	24.224085791795027	26.827090060870578	29.359532559217065
11	39.080282908245835	19.81984850012292	19.343028900998032	21.756839690633218
12	27.248177583481002	22.059993845696763	24.75320890322535	25.938619667596885
13	31.458017602526674	17.42391955960084	23.19628921619772	27.921773621674767
14	25.776473292430644	17.49297169227505	22.179924677758724	34.55063033753558
15	27.51970063523054	24.19729637961037	20.366460110634808	27.916542874524286
16	28.410143789740218	23.271411091543072	21.118455020617173	27.19999009809954
17	26.57404362018539	23.60013297543137	22.077811846837765	27.74801155754547
18	27.419928039109948	22.52588028324655	23.353518922885154	26.700672754758347
19	27.531763611903198	22.646699536649916	22.987361733046	26.83417511840089
20	28.52155251658158	21.344804910127138	22.494555302569612	27.639087270721667
21	29.36052266877846	21.363874197471507	22.16481177701728	27.110791356732754
22	29.343596579467967	21.444621430831816	22.144356069110728	27.06742592058949
23	27.18203783536332	21.65290658521971	23.06461847368263	28.10043710573434
24	26.97157342280313	22.4335030926122	22.841314258542024	27.75360922604264
25	27.24611653574484	22.15589713857445	22.463640027433485	28.134346298247227
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	169.0
1	169.0
2	278.5
3	388.0
4	388.0
5	388.0
6	778.5
7	1169.0
8	1169.0
9	1169.0
10	1131.0
11	1093.0
12	1093.0
13	1093.0
14	826.0
15	559.0
16	559.0
17	559.0
18	804.5
19	1050.0
20	1050.0
21	1050.0
22	1522.0
23	1994.0
24	1994.0
25	1994.0
26	2882.5
27	3771.0
28	3771.0
29	3771.0
30	4970.5
31	6170.0
32	6170.0
33	6170.0
34	8315.5
35	10461.0
36	10461.0
37	10461.0
38	15865.0
39	21269.0
40	21269.0
41	21269.0
42	31262.0
43	41255.0
44	41255.0
45	41255.0
46	54053.0
47	66851.0
48	66851.0
49	66851.0
50	77024.5
51	87198.0
52	87198.0
53	87198.0
54	86887.5
55	86577.0
56	86577.0
57	86577.0
58	84921.0
59	83265.0
60	83265.0
61	83265.0
62	74560.0
63	65855.0
64	65855.0
65	65855.0
66	54536.0
67	43217.0
68	43217.0
69	43217.0
70	32996.5
71	22776.0
72	22776.0
73	22776.0
74	17245.0
75	11714.0
76	11714.0
77	11714.0
78	8594.0
79	5474.0
80	5474.0
81	5474.0
82	3879.0
83	2284.0
84	2284.0
85	2284.0
86	1699.0
87	1114.0
88	1114.0
89	1114.0
90	721.0
91	328.0
92	328.0
93	328.0
94	186.5
95	45.0
96	45.0
97	45.0
98	130.5
99	216.0
100	216.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.063867962180051E-4
2	1.7659669905450127E-4
3	3.5319339810900254E-4
4	0.012185172234760589
5	0.03496614641279125
6	0.068696115932201
7	0.11531764448258933
8	0.11690701477407985
9	0.12856239691167692
10	0.14127735924360102
11	0.14993059749727158
12	0.14198374603981903
13	0.1368624417672385
14	0.12220491574571489
15	0.14092416584549203
16	0.12609004312491393
17	0.13138794409654894
18	0.11955596525989737
19	0.10578142273364625
20	0.10136650525728373
21	0.0971281844799757
22	0.1093133567147363
23	0.08741536603197812
24	0.09871755477146621
25	0.09359625049888567
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	566262.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.29456026005811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.18100297738981	42.44803177347674
2	12.516165781553095	13.591194345028237
3	3.9812343083501434	6.484780981923829
4	1.741922366183498	3.783076355163718
5	0.9234344245574859	2.5068733005174253
6	0.5776079754221853	1.8816582616950004
7	0.3714011937495198	1.4115545146283575
8	0.26750316391114776	1.1619173322184013
9	0.1938319831996779	0.9471620062945333
>10	1.057510851531255	10.406527742947018
>50	0.09746306112347182	3.689638710308627
>100	0.08339959648317304	8.899668309302145
>500	0.00719525930443059	2.5531631539795283
>1k	3.270572411104814E-4	0.23475321251640954
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1322	0.23346083615005067	No Hit
TATCAACGCAGAGTACTTTTTTTTT	846	0.14940080740010805	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	843	0.14887101730294455	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	842	0.14869442060389007	No Hit
TCGTAGTTCCGACCATAAACGATGC	840	0.14834122720578108	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	838	0.1479880338076721	No Hit
ATCAGATACCGTCGTAGTTCCGACC	797	0.14074756914643752	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	767	0.13544966817480247	No Hit
GTACATGGGGAATAATTGCAATCCC	682	0.12043894875516986	No Hit
GTACATGGGGTGGTATCAACGCAAA	677	0.11955596525989737	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	659	0.11637722467691634	No Hit
GTATCAACGCAGAGTACATGGGGAA	653	0.11531764448258933	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCA	641	0.11319848409393532	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.7659669905450127E-4
11	0.0	0.0	0.0	0.0	1.7659669905450127E-4
12	0.0	0.0	0.0	0.0	0.0015893702914905115
13	0.0	0.0	0.0	0.0	0.0022957570877085165
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACA	40	2.75683E-4	16.625309	4
AATTTCA	110	1.2732926E-11	14.680794	19
GATAACG	65	3.3592332E-6	14.616949	11
ATAACGA	65	3.3592332E-6	14.616949	12
TAACGAA	65	3.365405E-6	14.614365	13
GCCGCGC	40	0.0052667735	14.252787	8
ACCGTCC	40	0.0052667735	14.252787	8
CTACACT	60	2.5621153E-5	14.250265	4
CCGATAA	70	7.228413E-6	13.574081	9
CGATAAC	70	7.228413E-6	13.574081	10
CAAGACG	105	1.3624231E-9	13.571681	4
AACGAAC	70	7.2548755E-6	13.569282	14
GACCGTC	50	0.0014952862	13.3026	7
GTCCTAA	50	0.0015012769	13.295546	1
AAGAATT	125	1.0186341E-10	12.919098	16
AAGACGG	105	1.9826984E-8	12.666903	5
CGAACGA	75	1.47570845E-5	12.665783	16
CCAGAAC	70	1.089246E-4	12.213433	3
GAATTTC	140	5.2750693E-11	12.213433	18
ATAACGC	55	0.0030659009	12.090065	3
>>END_MODULE