FastQCFastQC Report
Thu 2 Feb 2017
SRR4062295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566262
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9500.16776686410177621No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9110.16087959283865066No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8330.14710505031239957No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8210.14498588992374553No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT7050.12450067283342339No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT6720.11867298176462485No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6130.10825377652040928No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5960.10525163263648277No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA5700.10066011846106572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTAG300.005742748329.3330444
ACGCTTA508.3343126E-526.39973417
ATATCAA602.8618E-422.0094971
TTAGGGG500.002578910221.9997794
TCTTAGG500.002578910221.9997792
TATTGGG500.002578910221.99977919
TTTATTA500.002578910221.9997793
TTATTCT500.002578910221.9997794
GTATCAA16600.020.1532731
CTTAGAC550.004480980319.99983
TATTGGA708.116589E-418.8569552
TAGCACA951.5948053E-518.526134
TTAGGAC600.00740778718.333153
ATGCGCA600.00740778718.3331511
ATTATTC600.00740778718.333153
GTAACAC600.00740778718.333153
CGCTTAC600.00740778718.3331518
CTAGGCC851.4292418E-418.1174663
GGTATCA8950.017.7059631
CGGTCCA1751.9099389E-1017.59982310
Produced by FastQC (version 0.11.5)