##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062295_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	566262
Sequences flagged as poor quality	0
Sequence length	50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.272716516382875	32.0	32.0	32.0	32.0	32.0
2	31.5146433982856	32.0	32.0	32.0	32.0	32.0
3	31.584987161419978	32.0	32.0	32.0	32.0	32.0
4	31.665688320953905	32.0	32.0	32.0	32.0	32.0
5	31.654651027263	32.0	32.0	32.0	32.0	32.0
6	35.290084801734885	36.0	36.0	36.0	36.0	36.0
7	35.28555509640414	36.0	36.0	36.0	36.0	36.0
8	35.258428077462376	36.0	36.0	36.0	36.0	36.0
9	35.285738756971156	36.0	36.0	36.0	36.0	36.0
10	35.183897206593414	36.0	36.0	36.0	36.0	36.0
11	35.289240669513404	36.0	36.0	36.0	36.0	36.0
12	35.211804782944995	36.0	36.0	36.0	36.0	36.0
13	35.24120460140359	36.0	36.0	36.0	36.0	36.0
14	35.203725483963254	36.0	36.0	36.0	36.0	36.0
15	35.19894324535286	36.0	36.0	36.0	36.0	36.0
16	35.205394322769315	36.0	36.0	36.0	36.0	36.0
17	35.17741080983714	36.0	36.0	36.0	36.0	36.0
18	35.182081792527136	36.0	36.0	36.0	36.0	36.0
19	35.16279213508941	36.0	36.0	36.0	36.0	36.0
20	35.155375073729125	36.0	36.0	36.0	36.0	36.0
21	35.13462496159022	36.0	36.0	36.0	36.0	36.0
22	35.13939307246469	36.0	36.0	36.0	36.0	36.0
23	35.11056189537705	36.0	36.0	36.0	36.0	36.0
24	35.090901384871316	36.0	36.0	36.0	36.0	36.0
25	35.06976275999449	36.0	36.0	36.0	36.0	36.0
26	35.02785812927585	36.0	36.0	36.0	36.0	36.0
27	35.035940960191574	36.0	36.0	36.0	36.0	36.0
28	35.006943782206825	36.0	36.0	36.0	36.0	36.0
29	34.997582391189944	36.0	36.0	36.0	36.0	36.0
30	34.96730488713705	36.0	36.0	36.0	36.0	36.0
31	34.96321137565297	36.0	36.0	36.0	36.0	36.0
32	34.91907986055924	36.0	36.0	36.0	32.0	36.0
33	34.89118111404262	36.0	36.0	36.0	32.0	36.0
34	34.86569114650109	36.0	36.0	36.0	32.0	36.0
35	34.84353885657169	36.0	36.0	36.0	32.0	36.0
36	34.820537136519846	36.0	36.0	36.0	32.0	36.0
37	34.80577895037986	36.0	36.0	36.0	32.0	36.0
38	34.786715336716924	36.0	36.0	36.0	32.0	36.0
39	34.75450056687541	36.0	36.0	36.0	32.0	36.0
40	34.743544507666066	36.0	36.0	36.0	32.0	36.0
41	34.706614605959786	36.0	36.0	36.0	32.0	36.0
42	34.66837612271351	36.0	36.0	36.0	32.0	36.0
43	34.64001999074633	36.0	36.0	36.0	32.0	36.0
44	34.59244837195503	36.0	36.0	36.0	32.0	36.0
45	34.564162878667474	36.0	36.0	36.0	32.0	36.0
46	34.524439217182156	36.0	36.0	36.0	32.0	36.0
47	34.488364043499296	36.0	36.0	36.0	32.0	36.0
48	34.44373452571425	36.0	36.0	36.0	32.0	36.0
49	34.416870635854075	36.0	36.0	36.0	32.0	36.0
50	33.994633226315734	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	32.0
22	62.0
23	187.0
24	455.0
25	988.0
26	2034.0
27	3582.0
28	5804.0
29	8441.0
30	11691.0
31	16145.0
32	23045.0
33	36383.0
34	81198.0
35	376212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.76083749803468	15.361785795674363	10.969549540955398	27.907827165335554
2	18.2048891102328	17.969475732819063	33.64980803125188	30.175827125696266
3	20.901566244421723	20.572211655797084	25.535311580036694	32.990910519744496
4	14.810105569506696	14.24693869622189	29.96687045925738	40.97608527501404
5	17.144537334308147	34.12024822432019	29.65164535144509	19.083569089926574
6	38.48556932428447	30.032900139335535	15.292118977358419	16.18941155902158
7	33.86594897768171	27.58157884512823	17.465413536490175	21.087058640699887
8	31.181325958655183	29.028082407083645	17.373583252981835	22.417008381279338
9	28.636567525279816	13.336052922498773	16.95628525311605	41.07109429910536
10	19.54925458533329	23.838965002066182	27.241100409351148	29.370680003249376
11	39.32720189594216	19.584397328445135	19.342989640837633	21.74541113477507
12	27.267589914209324	21.709562004867006	25.01227347058429	26.010574610339383
13	31.65531149891746	17.10233072323412	23.394647707245056	27.84771007060336
14	25.932518869357295	17.19998869781126	22.35519953661026	34.51229289622118
15	27.62060671561927	23.787398765942267	20.622432725487492	27.969561792950966
16	28.62193558413303	22.890449229856355	21.27846317403021	27.209152011980404
17	26.63766948868192	23.08525029050157	22.4901194146879	27.78696080612861
18	27.529306222208096	21.953971836358434	23.73300698263348	26.783714958799994
19	27.526304078324166	22.130921728811046	23.479060929393107	26.863713263471677
20	28.57387569711547	20.711967251908128	23.053286287972703	27.660870763003697
21	29.358848024412726	20.855540368239435	22.681373639763926	27.10423796758391
22	29.213685537789928	20.93165354553193	22.86291504639195	26.991745870286195
23	27.124016797878014	20.99575814728871	23.548640028820582	28.331585026012696
24	26.84231067409083	21.887510838679827	23.379584253848503	27.89059423338084
25	27.25713851788669	21.451138083456513	22.95045200164589	28.34127139701091
26	27.172901446158033	22.554343507123065	23.432033751339933	26.840721295378973
27	27.918849854925043	21.389328911328562	23.28916626490705	27.402654968839347
28	27.422388383908846	21.70105377669541	23.329489323601454	27.547068515794287
29	26.603327093561262	21.824073747041993	23.622187687634653	27.950411471762088
30	26.438218756180948	22.04144415246814	23.854581673306775	27.665755418044135
31	27.96688441155019	21.788086370371154	22.223222942151914	28.021806275926743
32	27.67814727889535	21.757534979576484	22.367150014392706	28.197167727135465
33	26.97713598499632	21.642316882144453	22.944896434682946	28.435650698176286
34	27.8800187194815	21.933051363784866	22.96262284659738	27.224307070136245
35	27.607185366491127	21.69437468874832	23.703691930590434	26.994748014170117
36	26.961337479845792	22.216512232035164	22.85685525528071	27.965295032838327
37	28.423664459161145	22.019072847682118	22.43055187637969	27.126710816777045
38	27.215670484687298	22.094719405504872	22.722520670643625	27.967089439164205
39	28.24220012397154	21.983579951930125	22.22534211376773	27.54887781033061
40	28.003468359169432	22.095778985699198	22.341071800685903	27.559680854445467
41	26.991502076799183	23.15277895510158	22.812473510214463	27.043245457884773
42	28.353295633960517	22.5309677624445	22.419529735056532	26.696206868538447
43	27.706449562023174	21.473756710935294	22.72799519638316	28.09179853065838
44	27.040267591132334	22.42788796917192	22.714163857183475	27.81768058251227
45	27.609262307241302	22.346234538685955	22.47992060072194	27.564582553350803
46	27.546154851424998	21.971369334176313	22.699851301377848	27.782624513020842
47	27.486604951135003	22.130704595464227	22.873304206239602	27.509386247161167
48	27.59979937693824	22.455124718315332	22.172910235308247	27.772165669438188
49	27.384501271545638	22.649759819157957	22.1102359423566	27.855502966939817
50	27.311643382021817	22.965176343436593	22.22910960295133	27.494070671590258
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	8.0
2	12.0
3	19.5
4	27.0
5	41.5
6	56.0
7	70.5
8	85.0
9	123.0
10	161.0
11	264.5
12	368.0
13	647.5
14	927.0
15	1408.0
16	1889.0
17	2108.0
18	2327.0
19	2275.5
20	2224.0
21	2121.0
22	2018.0
23	1759.5
24	1501.0
25	1159.0
26	817.0
27	753.5
28	690.0
29	818.5
30	947.0
31	1233.0
32	1519.0
33	1871.0
34	2223.0
35	2364.0
36	2505.0
37	3137.5
38	3770.0
39	4570.0
40	5370.0
41	6749.0
42	8128.0
43	10325.0
44	12522.0
45	16312.0
46	20102.0
47	25262.5
48	30423.0
49	36508.0
50	42593.0
51	45406.5
52	48220.0
53	51918.0
54	55616.0
55	58599.5
56	61583.0
57	60786.5
58	59990.0
59	57364.0
60	54738.0
61	49025.5
62	43313.0
63	38460.5
64	33608.0
65	28621.0
66	23634.0
67	20183.5
68	16733.0
69	14183.0
70	11633.0
71	8644.0
72	5655.0
73	4689.5
74	3724.0
75	2773.0
76	1822.0
77	1508.5
78	1195.0
79	995.5
80	796.0
81	608.0
82	420.0
83	337.0
84	254.0
85	167.5
86	81.0
87	53.5
88	26.0
89	17.0
90	8.0
91	4.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03443635631562775
2	0.004238320777308031
3	5.297900971635038E-4
4	0.0
5	0.0
6	5.297900971635038E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.063867962180051E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	5.297900971635038E-4
25	5.297900971635038E-4
26	5.297900971635038E-4
27	5.297900971635038E-4
28	0.0022957570877085165
29	3.5319339810900254E-4
30	0.0010595801943270077
31	5.297900971635038E-4
32	5.297900971635038E-4
33	1.7659669905450127E-4
34	0.001236176893381509
35	0.0
36	5.297900971635038E-4
37	0.0021191603886540154
38	0.0
39	5.297900971635038E-4
40	0.0
41	0.0010595801943270077
42	0.004944707573526036
43	0.0038851273791990276
44	0.004414917476362532
45	0.0017659669905450129
46	0.0028255471848720203
47	0.0014127735924360102
48	0.004591514175417033
49	0.0038851273791990276
50	0.0015893702914905113
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	566262.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.21650889209495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.81150603704499	45.0931923596798
2	12.415534158789185	14.207470411929402
3	3.73012296276308	6.40273841002624
4	1.6780468096452585	3.840479208216779
5	0.9049577499388826	2.588926157317416
6	0.5407665772612633	1.8564465405850095
7	0.3546009762791167	1.4202320938693742
8	0.25846250936537346	1.1830657972301653
9	0.19174330285362015	0.9873794153451432
>10	0.9551870559208501	9.770353581327102
>50	0.08930350371760762	3.562373382013296
>100	0.06666754057991647	7.807982474659031
>500	0.0031008158409262837	1.279360167801261
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	950	0.16776686410177621	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	911	0.16087959283865066	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	833	0.14710505031239957	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	821	0.14498588992374553	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	705	0.12450067283342339	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT	672	0.11867298176462485	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG	613	0.10825377652040928	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	596	0.10525163263648277	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA	570	0.10066011846106572	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.5319339810900254E-4
2	0.0	0.0	0.0	0.0	3.5319339810900254E-4
3	0.0	0.0	0.0	0.0	3.5319339810900254E-4
4	0.0	0.0	0.0	0.0	3.5319339810900254E-4
5	0.0	0.0	0.0	0.0	3.5319339810900254E-4
6	0.0	0.0	0.0	0.0	5.297900971635038E-4
7	0.0	0.0	0.0	0.0	5.297900971635038E-4
8	0.0	0.0	0.0	0.0	5.297900971635038E-4
9	0.0	0.0	0.0	0.0	5.297900971635038E-4
10	0.0	0.0	0.0	0.0	7.063867962180051E-4
11	0.0	0.0	0.0	0.0	7.063867962180051E-4
12	0.0	0.0	0.0	0.0	0.0021191603886540154
13	0.0	0.0	0.0	0.0	0.002648950485817519
14	0.0	0.0	0.0	0.0	0.0028255471848720203
15	0.0	0.0	0.0	0.0	0.0030021438839265215
16	0.0	0.0	0.0	0.0	0.0030021438839265215
17	0.0	0.0	0.0	0.0	0.0030021438839265215
18	0.0	0.0	0.0	0.0	0.0030021438839265215
19	0.0	0.0	0.0	0.0	0.0030021438839265215
20	0.0	0.0	0.0	0.0	0.003885127379199028
21	0.0	0.0	0.0	0.0	0.004591514175417033
22	0.0	0.0	0.0	0.0	0.004591514175417033
23	0.0	0.0	0.0	3.5319339810900254E-4	0.004591514175417033
24	0.0	0.0	0.0	0.0010595801943270077	0.004591514175417033
25	0.0	0.0	0.0	0.001942563689599514	0.004591514175417033
26	0.0	0.0	0.0	0.0022957570877085165	0.004944707573526035
27	0.0	0.0	0.0	0.0028255471848720203	0.005121304272580537
28	0.0	0.0	0.0	0.004414917476362532	0.005121304272580537
29	0.0	0.0	0.0	0.007770254758398056	0.005121304272580537
30	0.0	0.0	0.0	0.013068155730033094	0.005121304272580537
31	0.0	0.0	0.0	0.025606521362902684	0.005297900971635038
32	0.0	0.0	0.0	0.037791693597663274	0.005297900971635038
33	0.0	0.0	0.0	0.04927047903620586	0.005297900971635038
34	0.0	0.0	0.0	0.06092586117380294	0.005651094369744041
35	0.0	0.0	0.0	0.08176427166223409	0.005651094369744041
36	0.0	0.0	0.0	0.10595801943270076	0.005651094369744041
37	0.0	0.0	0.0	0.138451812058729	0.005651094369744041
38	0.0	0.0	0.0	0.18401376041479034	0.006004287767853043
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTTAG	30	0.0057427483	29.33304	44
ACGCTTA	50	8.3343126E-5	26.399734	17
ATATCAA	60	2.8618E-4	22.009497	1
TTAGGGG	50	0.0025789102	21.999779	4
TCTTAGG	50	0.0025789102	21.999779	2
TATTGGG	50	0.0025789102	21.999779	19
TTTATTA	50	0.0025789102	21.999779	3
TTATTCT	50	0.0025789102	21.999779	4
GTATCAA	1660	0.0	20.153273	1
CTTAGAC	55	0.0044809803	19.9998	3
TATTGGA	70	8.116589E-4	18.856955	2
TAGCACA	95	1.5948053E-5	18.52613	4
TTAGGAC	60	0.007407787	18.33315	3
ATGCGCA	60	0.007407787	18.33315	11
ATTATTC	60	0.007407787	18.33315	3
GTAACAC	60	0.007407787	18.33315	3
CGCTTAC	60	0.007407787	18.33315	18
CTAGGCC	85	1.4292418E-4	18.117466	3
GGTATCA	895	0.0	17.705963	1
CGGTCCA	175	1.9099389E-10	17.599823	10
>>END_MODULE