FastQCFastQC Report
Thu 2 Feb 2017
SRR4062294_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062294_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190816
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13940.730546704678853No Hit
TATCAACGCAGAGTACTTTTTTTTT9430.49419335904745937No Hit
CCATAGGGTCTTCTCGTCTTATTAT8320.4360221365084689No Hit
GTATTAGAGGCACTGCCTGCCCAGT6440.3374979037397283No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6280.32911286265302697No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA5510.28875985242327684No Hit
GGTATCAACGCAGAGTACTTTTTTT5080.26622505450276707No Hit
CTCTAATACTTGTAATGCTAGAGGT4950.2594122086198222No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4930.25836407848398457No Hit
CCATTGGGATGTCCTGATCCAACAT4760.24945497232936442No Hit
GTTATATAATTTAAGCTCCATAGGG4670.2447383867180949No Hit
GTCAGGATACCGCGGCCGTTAAACT4650.24369025658225726No Hit
CTGTTAGTATGAGTAACAAGAATTC4630.2426421264464196No Hit
ATCCTGACCGTGCAAAGGTAGCATA4550.23844960590306893No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4500.23582928056347474No Hit
ATCTATAACTTTATAGATGCAACAC4470.23425708535971826No Hit
GTTCATGCTAGTCCCTAATTAAGGA4470.23425708535971826No Hit
ACCTATAACTTCTCTGTTAACCCAA4420.2316367600201241No Hit
GGTCAGGATACCGCGGCCGTTAAAC4380.22954049974844878No Hit
GATTAAACCTTGTACCTTTTGCATA4160.21801106825423444No Hit
GCCTAAAGGAAAGATCCAAAAAGAT4130.21643887305047796No Hit
GTTATAGATTAACCCAATTTTAAGT4080.2138185477108838No Hit
CTTCTACACCATTGGGATGTCCTGA4030.21119822237128963No Hit
ATAAATAATCCACCTATAACTTCTC4010.21015009223545197No Hit
GACTATAGGCAATAATCACACTATA4010.21015009223545197No Hit
CCTATAACTTCTCTGTTAACCCAAC3940.20648163676002013No Hit
CTATAGAACTAGTACCGCAAGGGAA3920.20543350662418244No Hit
CACTATAAATAATCCACCTATAACT3790.19862066074123763No Hit
CTGTTAACCCAACACCGGAATGCCT3680.19285594499413047No Hit
CCTCTAGCATTACAAGTATTAGAGG3660.1918078148582928No Hit
GTACATGGGGTGGTATCAACGCAAA3620.18971155458661748No Hit
AAATATATCTGGGTCAATAAGATAT3500.18342277377159147No Hit
GTATCCTGACCGTGCAAAGGTAGCA3400.17818212309240314No Hit
CTATTAAAGGTTTTTTCCGTTCCAG3380.17713399295656548No Hit
GATTAAAGATAAGAGACAGTTGGAC3330.17451366761697132No Hit
GTATAGGGGTCCTAGGAAGATAATA3180.16665269159818885No Hit
GTACATGGGAGAAATCGTAAATAGA3160.16560456146235117No Hit
TGCTAGAGGTGATGTTTTTGGTAAA3040.15931578064732516No Hit
GTATCAACGCAGAGTACATGGGGTG3040.15931578064732516No Hit
ATCGTAAATAGATAGAAACCGACCT3010.15774358544356867No Hit
TACTAACAGTGTTGCATCTATAAAG2990.156695455307731No Hit
GTTTAAAATTGAACTTAAATTCATT2910.15250293476438034No Hit
GATATATTTTGATCAACGGACCAAG2910.15250293476438034No Hit
ACCCTATGGAGCTTAAATTATATAA2840.14883447928894852No Hit
CTTTATTGGTGGCTGCTTTTAGGCC2810.147262284085192No Hit
AGCATGAACGGCTAAACGAGGGTCC2800.14673821901727319No Hit
GTCCTGATCCAACATCGAGGTCGTA2790.14621415394935436No Hit
GTTATCCCTAGGGTAACTTGGTCCG2720.1425456984739225No Hit
AATCTAAACTTACTTTTTGATTTTG2660.13940130806640955No Hit
ACCTTTGCACGGTCAGGATACCGCG2640.13835317793057186No Hit
GTTAGTATGAGTAACAAGAATTCCA2630.13782911286265304No Hit
GATAGAAACCGACCTGGATTGCTCC2620.13730504779473418No Hit
CTTAAATAGGATTGCGCTGTTATCC2600.13625691765889653No Hit
GTATCAACGCAGAGTACATGGGAGA2580.13520878752305887No Hit
GCTATCACCAAGCTCGTTAGGCTTT2560.13416065738722122No Hit
GACCTGGATTGCTCCGGTCTGAACT2460.12892000670803286No Hit
GGATACCGCGGCCGTTAAACTTTAG2390.12525155123260104No Hit
CGTCTATGTGGCAAAATAGTGAGAA2350.12315529096092571No Hit
GGATTGCTCCGGTCTGAACTCAGAT2290.12001090055341272No Hit
GGGCAGGCAGTGCCTCTAATACTTG2280.11948683548549388No Hit
CCTATAGTCTGATTAACTAACAATG2220.11634244507798087No Hit
TCTAGGAGCTATAGAACTAGTACCG2180.11424618480630555No Hit
ATCCTAGCCCTAGCCCTACACAAAT2170.11372211973838672No Hit
GTTATACGCGTATGCCTGGAGAATT2150.11267398960254904No Hit
CATTTACACCTACTACCCAACTATC2120.11110179439879256No Hit
CTATTATATAAATCAAAACATTTAT2120.11110179439879256No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2070.1084814690591984No Hit
GTTTAGATTATAGCCAAAAGAGGGA2040.1069092738554419No Hit
GGTTATAATCATTCGGAGGATTTTT2040.1069092738554419No Hit
CTCTTAAATAGGATTGCGCTGTTAT2030.10638520878752307No Hit
CTATAGTATAAGTTTGAAATTTCGG2030.10638520878752307No Hit
TTATATAACTTATCTATTTAATTTA2030.10638520878752307No Hit
CCAATAAAGAAAGCGTTCAAGCTCA2010.10533707865168539No Hit
CCCTAATTGTCGATATGAACTCTTA2010.10533707865168539No Hit
CAGTTGGACCCTCGTTTAGCCGTTC1960.10271675331209122No Hit
ATTTCAGCCTCTTCACTGAAAGGTC1950.1021926882441724No Hit
CCATTAATAGCTTCTACACCATTGG1940.10166862317625355No Hit
GCGGTATCCTGACCGTGCAAAGGTA1930.10114455810833474No Hit
GTATTGGAATTAGTGAAATTGGAGT1930.10114455810833474No Hit
ATTTTAAACTTGCTAAAAAAACAAC1920.1006204930404159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC250.006014060718.9994743
CATCTAG453.5135017E-516.888421
TATGCCT453.5135017E-516.8884211
ACTGCTC350.002161215316.2852638
CAGCTAG350.002161215316.2852637
AGGCTTA350.002161215316.2852636
GTATGCC508.652218E-515.19957910
GCTGCTT400.005257609314.24960512
GGCTTAC400.005257609314.2496057
CTAAAGT400.005257609314.24960516
TATAGGG400.005257609314.2496052
AGCTAGA400.005257609314.2496058
TATATCT551.9464434E-413.81780054
GCGTATG551.9464434E-413.81780058
TTTGGTA852.6581802E-713.41139417
GACCTTT500.001488505413.303122519
TTAAAGA500.001491454713.2996323
ATCCAAA655.4050928E-513.15348214
TATGGAG801.9739055E-613.0621395
GTTATAT1102.6830094E-912.9541881
Produced by FastQC (version 0.11.5)