##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062294_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	190816
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.029001760858627	32.0	32.0	32.0	32.0	32.0
2	30.719703798423613	32.0	32.0	32.0	32.0	32.0
3	30.786752683213148	32.0	32.0	32.0	32.0	32.0
4	30.73687741069931	32.0	32.0	32.0	32.0	32.0
5	30.80281527754486	32.0	32.0	32.0	32.0	32.0
6	34.35207215327855	36.0	36.0	36.0	32.0	36.0
7	34.22959814690592	36.0	36.0	36.0	32.0	36.0
8	34.12152020794902	36.0	36.0	36.0	32.0	36.0
9	34.18128982894516	36.0	36.0	36.0	32.0	36.0
10	33.993648331376825	36.0	36.0	36.0	32.0	36.0
11	34.339211596511824	36.0	36.0	36.0	32.0	36.0
12	34.149358544356865	36.0	36.0	36.0	32.0	36.0
13	34.27766539493543	36.0	36.0	36.0	32.0	36.0
14	34.13853136005366	36.0	36.0	36.0	32.0	36.0
15	34.14766057353681	36.0	36.0	36.0	32.0	36.0
16	34.17078756498407	36.0	36.0	36.0	32.0	36.0
17	34.091548926714744	36.0	36.0	36.0	32.0	36.0
18	34.14986688747275	36.0	36.0	36.0	32.0	36.0
19	34.09635460338755	36.0	36.0	36.0	32.0	36.0
20	34.027843577058526	36.0	36.0	36.0	32.0	36.0
21	33.9838587959081	36.0	36.0	36.0	32.0	36.0
22	33.99044105316116	36.0	36.0	36.0	32.0	36.0
23	34.01200109005534	36.0	36.0	36.0	32.0	36.0
24	34.00153026999832	36.0	36.0	36.0	32.0	36.0
25	33.68872631225893	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2.0
5	14.0
6	44.0
7	6.0
8	31.0
9	32.0
10	17.0
11	2.0
12	10.0
13	12.0
14	63.0
15	85.0
16	119.0
17	148.0
18	182.0
19	285.0
20	394.0
21	523.0
22	720.0
23	1044.0
24	1430.0
25	1746.0
26	2314.0
27	2839.0
28	3748.0
29	4907.0
30	6082.0
31	8369.0
32	11768.0
33	17259.0
34	38934.0
35	87687.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.877053046422176	24.007672393010996	12.807236366304359	23.308038194262476
2	14.323746436357537	23.31146235116552	40.94415562636257	21.420635586114372
3	15.255534127117224	29.31619989937951	32.55177762871038	22.87648834479289
4	9.24895178197065	19.816037735849058	43.90670859538784	27.028301886792455
5	9.491855960325656	42.003533365137116	37.67332623864369	10.831284435893537
6	24.7194428712269	44.97566756864473	19.60438822813752	10.700501331990854
7	24.615699400858315	36.63420878671186	23.20913297587694	15.54095883655289
8	23.716889371547285	36.617508958034946	21.539680291273854	18.12592137914391
9	25.013248542660303	16.6281016018931	24.145403411565322	34.213246443881275
10	14.076857279296393	28.0151762427779	35.93337566448539	21.974590813440315
11	28.81126441245467	27.28513175227109	27.422105832156895	16.48149800311734
12	20.57148854661384	31.43711789247199	30.57647398388917	17.414919577025007
13	23.029170842800696	23.942760289032204	31.059417422743707	21.968651445423397
14	19.131867382327787	26.251226435388496	30.32010619487599	24.29679998740772
15	21.693771417476135	31.194865578278414	26.45350210173334	20.65786090251211
16	21.105712216776684	31.170568912863246	27.206754331650696	20.516964538709374
17	17.414005719833128	31.72041035867027	29.20735706976622	21.65822685173038
18	17.169300509979223	31.31020588050116	31.645994669352977	19.874498940166635
19	20.036197670758575	30.954779141747977	31.1053404679467	17.903682719546744
20	19.01287283485632	31.77187699990558	30.793037968043475	18.422212197194625
21	21.58878700770054	28.482028578023037	29.99118739377662	19.9379970204998
22	19.325967662396256	30.36293241839529	30.320438162989078	19.990661756219374
23	20.345641455994965	30.501416133431235	30.633064093150107	18.519878317423686
24	19.409240647097086	31.778887513376276	29.903061331542837	18.9088105079838
25	19.493605816137052	30.703622496616696	31.725957553949286	18.076814133296963
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	647.0
1	647.0
2	468.0
3	289.0
4	289.0
5	289.0
6	885.0
7	1481.0
8	1481.0
9	1481.0
10	1405.0
11	1329.0
12	1329.0
13	1329.0
14	1583.5
15	1838.0
16	1838.0
17	1838.0
18	3447.5
19	5057.0
20	5057.0
21	5057.0
22	7043.0
23	9029.0
24	9029.0
25	9029.0
26	13087.0
27	17145.0
28	17145.0
29	17145.0
30	19889.5
31	22634.0
32	22634.0
33	22634.0
34	23627.5
35	24621.0
36	24621.0
37	24621.0
38	24068.0
39	23515.0
40	23515.0
41	23515.0
42	21496.5
43	19478.0
44	19478.0
45	19478.0
46	21023.5
47	22569.0
48	22569.0
49	22569.0
50	19929.5
51	17290.0
52	17290.0
53	17290.0
54	14117.0
55	10944.0
56	10944.0
57	10944.0
58	8424.0
59	5904.0
60	5904.0
61	5904.0
62	4653.5
63	3403.0
64	3403.0
65	3403.0
66	2553.0
67	1703.0
68	1703.0
69	1703.0
70	1349.0
71	995.0
72	995.0
73	995.0
74	741.0
75	487.0
76	487.0
77	487.0
78	370.0
79	253.0
80	253.0
81	253.0
82	168.5
83	84.0
84	84.0
85	84.0
86	54.0
87	24.0
88	24.0
89	24.0
90	20.0
91	16.0
92	16.0
93	16.0
94	13.0
95	10.0
96	10.0
97	10.0
98	40.5
99	71.0
100	71.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010481301358376658
2	0.0
3	0.0
4	0.008385041086701326
5	0.03301609927888646
6	0.06498406842193527
7	0.11005366426295488
8	0.10743333892336071
9	0.12001090055341272
10	0.13363659231930236
11	0.14097350327016603
12	0.13678098272681538
13	0.13049220191178937
14	0.11686651014589972
15	0.1357328525909777
16	0.12682374643635752
17	0.13154033204762702
18	0.1152943149421432
19	0.10271675331209122
20	0.09537984236122757
21	0.0943317122253899
22	0.10586114371960423
23	0.08175415059533792
24	0.0943317122253899
25	0.0911873218178769
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	190816.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.431053999664595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.58487401153896	20.068023645815863
2	13.000682015078063	7.392461848063056
3	4.76673241046248	4.065696796914304
4	2.4994930969014395	2.842528928391749
5	1.406425688005751	1.999308234110347
6	0.9824703692097841	1.6759600872044274
7	0.7741792778013308	1.5407512996813684
8	0.5880075943300584	1.3374140533288612
9	0.4940001105970397	1.2640449438202246
>10	3.985179996682089	23.583452121415394
>50	0.5456120624504618	10.7134621834647
>100	0.35944037897918935	20.634537984236122
>500	0.011059703968590441	2.1518111688747275
>1k	0.0018432839947650735	0.730546704678853
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1394	0.730546704678853	No Hit
TATCAACGCAGAGTACTTTTTTTTT	943	0.49419335904745937	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	832	0.4360221365084689	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	644	0.3374979037397283	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	628	0.32911286265302697	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	551	0.28875985242327684	No Hit
GGTATCAACGCAGAGTACTTTTTTT	508	0.26622505450276707	No Hit
CTCTAATACTTGTAATGCTAGAGGT	495	0.2594122086198222	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	493	0.25836407848398457	No Hit
CCATTGGGATGTCCTGATCCAACAT	476	0.24945497232936442	No Hit
GTTATATAATTTAAGCTCCATAGGG	467	0.2447383867180949	No Hit
GTCAGGATACCGCGGCCGTTAAACT	465	0.24369025658225726	No Hit
CTGTTAGTATGAGTAACAAGAATTC	463	0.2426421264464196	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	455	0.23844960590306893	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	450	0.23582928056347474	No Hit
ATCTATAACTTTATAGATGCAACAC	447	0.23425708535971826	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	447	0.23425708535971826	No Hit
ACCTATAACTTCTCTGTTAACCCAA	442	0.2316367600201241	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	438	0.22954049974844878	No Hit
GATTAAACCTTGTACCTTTTGCATA	416	0.21801106825423444	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	413	0.21643887305047796	No Hit
GTTATAGATTAACCCAATTTTAAGT	408	0.2138185477108838	No Hit
CTTCTACACCATTGGGATGTCCTGA	403	0.21119822237128963	No Hit
ATAAATAATCCACCTATAACTTCTC	401	0.21015009223545197	No Hit
GACTATAGGCAATAATCACACTATA	401	0.21015009223545197	No Hit
CCTATAACTTCTCTGTTAACCCAAC	394	0.20648163676002013	No Hit
CTATAGAACTAGTACCGCAAGGGAA	392	0.20543350662418244	No Hit
CACTATAAATAATCCACCTATAACT	379	0.19862066074123763	No Hit
CTGTTAACCCAACACCGGAATGCCT	368	0.19285594499413047	No Hit
CCTCTAGCATTACAAGTATTAGAGG	366	0.1918078148582928	No Hit
GTACATGGGGTGGTATCAACGCAAA	362	0.18971155458661748	No Hit
AAATATATCTGGGTCAATAAGATAT	350	0.18342277377159147	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	340	0.17818212309240314	No Hit
CTATTAAAGGTTTTTTCCGTTCCAG	338	0.17713399295656548	No Hit
GATTAAAGATAAGAGACAGTTGGAC	333	0.17451366761697132	No Hit
GTATAGGGGTCCTAGGAAGATAATA	318	0.16665269159818885	No Hit
GTACATGGGAGAAATCGTAAATAGA	316	0.16560456146235117	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	304	0.15931578064732516	No Hit
GTATCAACGCAGAGTACATGGGGTG	304	0.15931578064732516	No Hit
ATCGTAAATAGATAGAAACCGACCT	301	0.15774358544356867	No Hit
TACTAACAGTGTTGCATCTATAAAG	299	0.156695455307731	No Hit
GTTTAAAATTGAACTTAAATTCATT	291	0.15250293476438034	No Hit
GATATATTTTGATCAACGGACCAAG	291	0.15250293476438034	No Hit
ACCCTATGGAGCTTAAATTATATAA	284	0.14883447928894852	No Hit
CTTTATTGGTGGCTGCTTTTAGGCC	281	0.147262284085192	No Hit
AGCATGAACGGCTAAACGAGGGTCC	280	0.14673821901727319	No Hit
GTCCTGATCCAACATCGAGGTCGTA	279	0.14621415394935436	No Hit
GTTATCCCTAGGGTAACTTGGTCCG	272	0.1425456984739225	No Hit
AATCTAAACTTACTTTTTGATTTTG	266	0.13940130806640955	No Hit
ACCTTTGCACGGTCAGGATACCGCG	264	0.13835317793057186	No Hit
GTTAGTATGAGTAACAAGAATTCCA	263	0.13782911286265304	No Hit
GATAGAAACCGACCTGGATTGCTCC	262	0.13730504779473418	No Hit
CTTAAATAGGATTGCGCTGTTATCC	260	0.13625691765889653	No Hit
GTATCAACGCAGAGTACATGGGAGA	258	0.13520878752305887	No Hit
GCTATCACCAAGCTCGTTAGGCTTT	256	0.13416065738722122	No Hit
GACCTGGATTGCTCCGGTCTGAACT	246	0.12892000670803286	No Hit
GGATACCGCGGCCGTTAAACTTTAG	239	0.12525155123260104	No Hit
CGTCTATGTGGCAAAATAGTGAGAA	235	0.12315529096092571	No Hit
GGATTGCTCCGGTCTGAACTCAGAT	229	0.12001090055341272	No Hit
GGGCAGGCAGTGCCTCTAATACTTG	228	0.11948683548549388	No Hit
CCTATAGTCTGATTAACTAACAATG	222	0.11634244507798087	No Hit
TCTAGGAGCTATAGAACTAGTACCG	218	0.11424618480630555	No Hit
ATCCTAGCCCTAGCCCTACACAAAT	217	0.11372211973838672	No Hit
GTTATACGCGTATGCCTGGAGAATT	215	0.11267398960254904	No Hit
CATTTACACCTACTACCCAACTATC	212	0.11110179439879256	No Hit
CTATTATATAAATCAAAACATTTAT	212	0.11110179439879256	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	207	0.1084814690591984	No Hit
GTTTAGATTATAGCCAAAAGAGGGA	204	0.1069092738554419	No Hit
GGTTATAATCATTCGGAGGATTTTT	204	0.1069092738554419	No Hit
CTCTTAAATAGGATTGCGCTGTTAT	203	0.10638520878752307	No Hit
CTATAGTATAAGTTTGAAATTTCGG	203	0.10638520878752307	No Hit
TTATATAACTTATCTATTTAATTTA	203	0.10638520878752307	No Hit
CCAATAAAGAAAGCGTTCAAGCTCA	201	0.10533707865168539	No Hit
CCCTAATTGTCGATATGAACTCTTA	201	0.10533707865168539	No Hit
CAGTTGGACCCTCGTTTAGCCGTTC	196	0.10271675331209122	No Hit
ATTTCAGCCTCTTCACTGAAAGGTC	195	0.1021926882441724	No Hit
CCATTAATAGCTTCTACACCATTGG	194	0.10166862317625355	No Hit
GCGGTATCCTGACCGTGCAAAGGTA	193	0.10114455810833474	No Hit
GTATTGGAATTAGTGAAATTGGAGT	193	0.10114455810833474	No Hit
ATTTTAAACTTGCTAAAAAAACAAC	192	0.1006204930404159	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	5.240650679188328E-4	0.0
9	0.0	0.0	0.0	5.240650679188328E-4	0.0
10	0.0	0.0	0.0	5.240650679188328E-4	0.0
11	0.0	0.0	0.0	5.240650679188328E-4	0.0
12	0.0	0.0	0.0	5.240650679188328E-4	0.0
13	0.0	0.0	0.0	5.240650679188328E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATAC	25	0.0060140607	18.999474	3
CATCTAG	45	3.5135017E-5	16.88842	1
TATGCCT	45	3.5135017E-5	16.88842	11
ACTGCTC	35	0.0021612153	16.285263	8
CAGCTAG	35	0.0021612153	16.285263	7
AGGCTTA	35	0.0021612153	16.285263	6
GTATGCC	50	8.652218E-5	15.199579	10
GCTGCTT	40	0.0052576093	14.249605	12
GGCTTAC	40	0.0052576093	14.249605	7
CTAAAGT	40	0.0052576093	14.249605	16
TATAGGG	40	0.0052576093	14.249605	2
AGCTAGA	40	0.0052576093	14.249605	8
TATATCT	55	1.9464434E-4	13.8178005	4
GCGTATG	55	1.9464434E-4	13.8178005	8
TTTGGTA	85	2.6581802E-7	13.411394	17
GACCTTT	50	0.0014885054	13.3031225	19
TTAAAGA	50	0.0014914547	13.299632	3
ATCCAAA	65	5.4050928E-5	13.153482	14
TATGGAG	80	1.9739055E-6	13.062139	5
GTTATAT	110	2.6830094E-9	12.954188	1
>>END_MODULE