FastQCFastQC Report
Thu 2 Feb 2017
SRR4062293_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062293_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192069
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC38440.3224645553235593No Hit
GTATCAACGCAGAGTACTTTTTTTT33900.2843795115886748No Hit
GAATGGAATGGAATGGAATGGAATG29360.24629446785379033No Hit
TATCAACGCAGAGTACTTTTTTTTT20800.17448654398361169No Hit
GGTATCAACGCAGAGTACTTTTTTT15110.1267544076727102No Hit
GTGTAAAGGTATAAGGGAGCTTGAC13520.11341625358934761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGTA504.565489E-617.09803418
GGTATCA10150.016.837571
AGTTCGG704.4223452E-714.93187510
ACGCATA456.7472004E-414.7816699
TAGTACT456.75796E-414.7785634
TTTAGAC653.373074E-614.6143183
CGAAATT1250.014.44076713
CCGTCTA400.005283641614.24776316
GTCCTAC2000.014.2417781
GCGAAAT1350.014.07541312
GTGCTAG551.9691612E-413.810211
AACGGAC1301.4551915E-1113.153439515
TAGGGTC1900.013.00068954
GTATCAA30800.012.6696351
TCCGACA604.0889424E-412.66733913
CGCATAG701.0882361E-412.21698910
AGGTATA2100.012.2164757
TTAGCGC701.090801E-412.21390715
CGGACCA1405.2750693E-1112.21134217
CGTCCGA550.003064569812.09256711
Produced by FastQC (version 0.11.5)