##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062293_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1192069
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37062787472873	32.0	32.0	32.0	32.0	32.0
2	31.132036820016292	32.0	32.0	32.0	32.0	32.0
3	31.148037571650633	32.0	32.0	32.0	32.0	32.0
4	31.129754234025043	32.0	32.0	32.0	32.0	32.0
5	31.151311710983173	32.0	32.0	32.0	32.0	32.0
6	34.77227744367146	36.0	36.0	36.0	36.0	36.0
7	34.7331412862846	36.0	36.0	36.0	32.0	36.0
8	34.688077619667986	36.0	36.0	36.0	32.0	36.0
9	34.73588189945381	36.0	36.0	36.0	32.0	36.0
10	34.59974212902105	36.0	36.0	36.0	32.0	36.0
11	34.729634777852624	36.0	36.0	36.0	32.0	36.0
12	34.68840478193796	36.0	36.0	36.0	32.0	36.0
13	34.684063590278754	36.0	36.0	36.0	32.0	36.0
14	34.62933605353381	36.0	36.0	36.0	32.0	36.0
15	34.605744298358566	36.0	36.0	36.0	32.0	36.0
16	34.58598453613004	36.0	36.0	36.0	32.0	36.0
17	34.601997032050996	36.0	36.0	36.0	32.0	36.0
18	34.579021851923	36.0	36.0	36.0	32.0	36.0
19	34.531012047121436	36.0	36.0	36.0	32.0	36.0
20	34.515018845385626	36.0	36.0	36.0	32.0	36.0
21	34.46296816711113	36.0	36.0	36.0	32.0	36.0
22	34.4850868531939	36.0	36.0	36.0	32.0	36.0
23	34.46301598313521	36.0	36.0	36.0	32.0	36.0
24	34.3953831531564	36.0	36.0	36.0	32.0	36.0
25	34.058464736521124	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	15.0
5	85.0
6	241.0
7	43.0
8	238.0
9	185.0
10	101.0
11	23.0
12	48.0
13	56.0
14	129.0
15	211.0
16	370.0
17	496.0
18	670.0
19	923.0
20	1240.0
21	1633.0
22	2267.0
23	3141.0
24	4431.0
25	6296.0
26	9086.0
27	12041.0
28	16766.0
29	22838.0
30	30451.0
31	43649.0
32	64748.0
33	98726.0
34	246041.0
35	624880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.67165188271041	20.484154294505803	11.72795283450973	23.11624098827406
2	15.663032645788663	21.026233402792457	40.44819590828711	22.862538043131767
3	18.148171450382318	27.299518063192863	29.26602156761586	25.286288918808957
4	11.512089044227638	15.680646874399617	39.32859430830398	33.47866977306877
5	14.065326919913938	37.31282538425132	32.9696694045657	15.652178291269045
6	30.55157292467623	39.6400858233637	17.066039668122798	12.742301583837271
7	27.24352433429325	32.51904088846562	22.15915150995988	18.07828326728125
8	26.157982081008356	35.1432612296043	20.1595374790458	18.53921921034155
9	26.40103276873741	14.210061263949015	21.245672299187124	38.143233668126456
10	16.777526692932568	27.096077756029537	30.873663253219537	25.252732297818365
11	34.64547149346661	25.44042491163777	21.25650377098997	18.65759982390565
12	21.63822078329017	27.35635157918966	30.963575772289566	20.04185186523061
13	27.009931530151178	23.38937091908865	27.05722453087107	22.543473019889102
14	21.437768402557012	21.21502525178291	27.449649717077563	29.897556628582517
15	25.526122136275966	27.449244292713086	23.333764558033884	23.690869012977064
16	24.59846694250278	29.920827306767727	23.101862817728097	22.37884293300139
17	20.510776622373044	28.71688477497606	27.262334738857994	23.5100038637929
18	20.484769079593843	29.650364920590928	28.530071304391647	21.334794695423582
19	23.602180921055393	25.539583146145727	28.24902403530855	22.60921189749033
20	24.92224472590846	26.09814630083062	26.586174003876007	22.39343496938491
21	27.117034434635993	26.331761163400152	24.24682368213562	22.304380719828234
22	23.179686660217133	27.257000429968826	27.984672283134472	21.578640626679565
23	22.846230315324238	28.08590562102654	27.669710535772456	21.398153527876758
24	22.284350145098884	26.57803431319862	27.36004003654342	23.77757550515907
25	23.703743509481363	26.510535967381156	26.137729980990255	23.64799054214723
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81.0
1	81.0
2	262.0
3	443.0
4	443.0
5	443.0
6	1004.5
7	1566.0
8	1566.0
9	1566.0
10	1810.0
11	2054.0
12	2054.0
13	2054.0
14	2840.5
15	3627.0
16	3627.0
17	3627.0
18	6772.5
19	9918.0
20	9918.0
21	9918.0
22	15728.5
23	21539.0
24	21539.0
25	21539.0
26	32867.5
27	44196.0
28	44196.0
29	44196.0
30	58924.0
31	73652.0
32	73652.0
33	73652.0
34	90882.5
35	108113.0
36	108113.0
37	108113.0
38	126557.5
39	145002.0
40	145002.0
41	145002.0
42	150760.5
43	156519.0
44	156519.0
45	156519.0
46	159760.0
47	163001.0
48	163001.0
49	163001.0
50	156791.0
51	150581.0
52	150581.0
53	150581.0
54	136373.5
55	122166.0
56	122166.0
57	122166.0
58	103806.5
59	85447.0
60	85447.0
61	85447.0
62	70418.0
63	55389.0
64	55389.0
65	55389.0
66	41568.0
67	27747.0
68	27747.0
69	27747.0
70	19768.5
71	11790.0
72	11790.0
73	11790.0
74	8502.0
75	5214.0
76	5214.0
77	5214.0
78	3777.5
79	2341.0
80	2341.0
81	2341.0
82	1596.0
83	851.0
84	851.0
85	851.0
86	565.0
87	279.0
88	279.0
89	279.0
90	188.0
91	97.0
92	97.0
93	97.0
94	72.5
95	48.0
96	48.0
97	48.0
98	228.0
99	408.0
100	408.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.227653768364079E-4
2	8.388776153058254E-5
3	3.3555104612233014E-4
4	0.01023430690673107
5	0.0336389923737636
6	0.0658518928015073
7	0.11257737597404177
8	0.11551344762761216
9	0.12440555034985391
10	0.1408475516098481
11	0.1508301952319874
12	0.14093143937137867
13	0.13573039815648255
14	0.12247613183465052
15	0.1393375719022976
16	0.1264188566265879
17	0.12826438738026072
18	0.11735897838128498
19	0.1003297627905767
20	0.09672258904476168
21	0.0961353747140476
22	0.10779577356679855
23	0.08732715975333642
24	0.097058140090884
25	0.09236042544517138
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1192069.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.31988592430524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.42525318935855	19.852571759516348
2	15.67643691043611	10.133213052897263
3	7.326349637942724	7.1036035361965135
4	4.24260559978763	5.484821160278192
5	2.6589704330303534	4.296881053582076
6	1.820794841765216	3.530872894644911
7	1.2422013055772543	2.8103463143895917
8	0.879338938199504	2.273610733712613
9	0.6529307070759324	1.8992381372253082
>10	3.588648193791686	21.147954552329935
>50	0.2900149010307256	6.536163538117214
>100	0.18264614706772345	10.99888644600966
>500	0.011724788153771308	2.487302995970126
>1k	0.002084406782892677	1.4445338251302278
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTCCATTCCATTCCATTCCATTCC	3844	0.3224645553235593	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3390	0.2843795115886748	No Hit
GAATGGAATGGAATGGAATGGAATG	2936	0.24629446785379033	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2080	0.17448654398361169	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1511	0.1267544076727102	No Hit
GTGTAAAGGTATAAGGGAGCTTGAC	1352	0.11341625358934761	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.388776153058254E-5	0.0
5	0.0	2.5166328459174763E-4	0.0	8.388776153058254E-5	0.0
6	0.0	3.3555104612233014E-4	0.0	8.388776153058254E-5	0.0
7	0.0	3.3555104612233014E-4	0.0	1.6777552306116507E-4	0.0
8	0.0	3.3555104612233014E-4	0.0	2.5166328459174763E-4	0.0
9	0.0	3.3555104612233014E-4	0.0	2.5166328459174763E-4	0.0
10	0.0	3.3555104612233014E-4	0.0	2.5166328459174763E-4	0.0
11	0.0	3.3555104612233014E-4	0.0	3.3555104612233014E-4	0.0
12	0.0	3.3555104612233014E-4	0.0	3.3555104612233014E-4	0.0
13	0.0	3.3555104612233014E-4	0.0	4.194388076529127E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACGTA	50	4.565489E-6	17.098034	18
GGTATCA	1015	0.0	16.83757	1
AGTTCGG	70	4.4223452E-7	14.931875	10
ACGCATA	45	6.7472004E-4	14.781669	9
TAGTACT	45	6.75796E-4	14.778563	4
TTTAGAC	65	3.373074E-6	14.614318	3
CGAAATT	125	0.0	14.440767	13
CCGTCTA	40	0.0052836416	14.247763	16
GTCCTAC	200	0.0	14.241778	1
GCGAAAT	135	0.0	14.075413	12
GTGCTAG	55	1.9691612E-4	13.81021	1
AACGGAC	130	1.4551915E-11	13.1534395	15
TAGGGTC	190	0.0	13.0006895	4
GTATCAA	3080	0.0	12.669635	1
TCCGACA	60	4.0889424E-4	12.667339	13
CGCATAG	70	1.0882361E-4	12.216989	10
AGGTATA	210	0.0	12.216475	7
TTAGCGC	70	1.090801E-4	12.213907	15
CGGACCA	140	5.2750693E-11	12.211342	17
CGTCCGA	55	0.0030645698	12.092567	11
>>END_MODULE