FastQCFastQC Report
Thu 2 Feb 2017
SRR4062293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192069
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGG12630.10595024281312576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.033.3186231
GTATCAA33150.026.6193731
AGGTCGC450.001398450524.44376
GCACGAA2150.022.51471943
ACGTACG602.8700795E-422.0030243
CGACCCG1407.2759576E-1222.00025237
TAGGACG2200.020.999364
CGCACGA1601.8189894E-1220.62696642
GCGTGCA755.4738382E-520.53270735
TACTCGT550.00448436819.999395
TAGGCCC1452.9649527E-1019.7235364
TGTCGGG2800.019.64225823
TGTCACG808.9905414E-519.2494135
CGTCTTA3200.019.24941315
GTCACGT808.9905414E-519.2494136
TAGGGTC3550.019.2106824
CGCTCCG1851.8189894E-1219.0296443
TCTCGTC3250.018.95326812
GGTCGCG708.124899E-418.8565677
TTGTCGG2800.018.85656722
Produced by FastQC (version 0.11.5)