##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062293_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1192069
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.309216161145034	32.0	32.0	32.0	32.0	32.0
2	31.516387893653807	32.0	32.0	32.0	32.0	32.0
3	31.61008465114016	32.0	32.0	32.0	32.0	32.0
4	31.68079532308952	32.0	32.0	32.0	32.0	32.0
5	31.661133709541982	32.0	32.0	32.0	32.0	32.0
6	35.31218578790322	36.0	36.0	36.0	36.0	36.0
7	35.3299699933477	36.0	36.0	36.0	36.0	36.0
8	35.27076536676988	36.0	36.0	36.0	36.0	36.0
9	35.352223738726536	36.0	36.0	36.0	36.0	36.0
10	35.25881974952792	36.0	36.0	36.0	36.0	36.0
11	35.3536800302667	36.0	36.0	36.0	36.0	36.0
12	35.304744943455454	36.0	36.0	36.0	36.0	36.0
13	35.323410809273625	36.0	36.0	36.0	36.0	36.0
14	35.28385521307911	36.0	36.0	36.0	36.0	36.0
15	35.27374422118183	36.0	36.0	36.0	36.0	36.0
16	35.27356302361692	36.0	36.0	36.0	36.0	36.0
17	35.27728260696319	36.0	36.0	36.0	36.0	36.0
18	35.25758240504535	36.0	36.0	36.0	36.0	36.0
19	35.2310419950523	36.0	36.0	36.0	36.0	36.0
20	35.24037115301211	36.0	36.0	36.0	36.0	36.0
21	35.207243037106075	36.0	36.0	36.0	36.0	36.0
22	35.20516010398727	36.0	36.0	36.0	36.0	36.0
23	35.185173005924995	36.0	36.0	36.0	36.0	36.0
24	35.13373890269774	36.0	36.0	36.0	36.0	36.0
25	35.13230945524126	36.0	36.0	36.0	36.0	36.0
26	35.07772452769093	36.0	36.0	36.0	36.0	36.0
27	35.08146256634473	36.0	36.0	36.0	36.0	36.0
28	35.06486872823637	36.0	36.0	36.0	36.0	36.0
29	35.00056120912464	36.0	36.0	36.0	36.0	36.0
30	35.001494041032856	36.0	36.0	36.0	36.0	36.0
31	34.957955453920874	36.0	36.0	36.0	36.0	36.0
32	34.98468545025498	36.0	36.0	36.0	36.0	36.0
33	34.941367487955816	36.0	36.0	36.0	36.0	36.0
34	34.87502904613743	36.0	36.0	36.0	32.0	36.0
35	34.87544261280178	36.0	36.0	36.0	32.0	36.0
36	34.819600207706095	36.0	36.0	36.0	32.0	36.0
37	34.84366844536684	36.0	36.0	36.0	32.0	36.0
38	34.833928237375524	36.0	36.0	36.0	32.0	36.0
39	34.72761811606543	36.0	36.0	36.0	32.0	36.0
40	34.75593694660292	36.0	36.0	36.0	32.0	36.0
41	34.67903703560784	36.0	36.0	36.0	32.0	36.0
42	34.722119273297096	36.0	36.0	36.0	32.0	36.0
43	34.68973775846868	36.0	36.0	36.0	32.0	36.0
44	34.565333885874054	36.0	36.0	36.0	32.0	36.0
45	34.57785245652727	36.0	36.0	36.0	32.0	36.0
46	34.50564019364651	36.0	36.0	36.0	32.0	36.0
47	34.53394644101977	36.0	36.0	36.0	32.0	36.0
48	34.4916636536979	36.0	36.0	36.0	32.0	36.0
49	34.354950090976274	36.0	36.0	36.0	32.0	36.0
50	34.021640525842045	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	4.0
21	18.0
22	53.0
23	149.0
24	440.0
25	1138.0
26	2473.0
27	4983.0
28	8773.0
29	14593.0
30	22359.0
31	34502.0
32	52824.0
33	88062.0
34	193350.0
35	768345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1804316759267	21.118655076316564	12.034596506951106	23.666316740805634
2	15.131294709034213	21.338273995333942	40.236054472560284	23.29437682307156
3	17.55670183521256	27.54932810097402	29.535286967449032	25.358683096364388
4	11.224098605030413	16.066184088337167	39.15704543948379	33.55267186714863
5	13.728651613287484	37.532139498636404	32.93232187062997	15.806887017446138
6	30.58477417796147	39.6627035225369	16.98419635809061	12.76832594141101
7	27.201697217191285	32.3766493382514	22.206097130283567	18.215556314273755
8	26.211653855607352	35.20953904513917	20.07551576292983	18.503291336323652
9	26.334129987442	13.938790455921596	21.283918967777872	38.44316058885853
10	16.664303828050222	27.107407373230913	30.924803849441602	25.303484949277266
11	34.842949527250525	25.30616935764624	21.263702017249	18.587179097854232
12	21.581301082403787	27.27400846763065	31.013305437856364	20.1313850121092
13	27.202787758091183	23.159397652317107	27.088029300317345	22.549785289274364
14	21.505885984787792	20.95096844226299	27.46627921705874	30.076866355890473
15	25.50892607726566	27.391199670488874	23.396045027594877	23.703829224650587
16	24.700014093297632	29.684511465904283	23.095643157704	22.51983128309408
17	20.616088498232905	28.559504525325295	27.280803376314626	23.543603600127174
18	20.575605230242928	29.479970505013558	28.51794404173591	21.426480223007598
19	23.653244904447646	25.457670654970478	28.246854838100816	22.642229602481066
20	25.067907541721823	25.974907345423308	26.50675887663561	22.450426236219258
21	27.30622136805839	26.23447132674367	24.14675660553206	22.312550699665877
22	23.217196320011677	27.08031162625653	27.948969396905714	21.753522656826075
23	22.930216287815554	27.987054440640602	27.627175943674402	21.455553327869445
24	22.37545887105975	26.40706476876925	27.344520727214157	23.87295563295684
25	23.90155267857817	26.228515295675	26.14630528937503	23.723626736371802
26	23.980759422312897	27.398361161816815	26.45914984430366	22.161729571566628
27	22.45475929225514	27.150800122140684	28.161229057402764	22.23321152820141
28	21.841132369558633	27.605073281383568	28.32752680468672	22.22626754437108
29	22.573406416780333	25.574089267168986	27.66794009036443	24.18456422568625
30	22.939900373640793	27.044664084009618	26.935776399391305	23.07965914295829
31	24.616463545581446	27.79666192419199	25.770512321486095	21.81636220874047
32	21.55569872556323	27.48704758836833	28.07787553604864	22.879378150019797
33	22.237247528024945	27.870506844017473	27.757593348673687	22.1346522792839
34	22.158401688508444	25.108006489617118	28.77636826396031	23.95722355791413
35	23.734695675151944	26.14656079995638	26.398812145310867	23.71993137958081
36	23.652781261455942	27.804141881286874	25.94334043304613	22.599736424211056
37	22.99049113078785	26.690435344303275	27.278919839435588	23.040153685473282
38	22.43712402553879	27.801830263181078	27.8518273690533	21.909218342226836
39	23.013464892375655	25.63237010124465	27.580421504568132	23.773743501811566
40	23.748541401546387	26.397213584113	26.76153813244032	23.092706881900295
41	24.512689043766546	27.562891551710834	25.997943042753167	21.926476361769453
42	22.272212481438913	27.850269712502413	27.581060561572468	22.296457244486202
43	22.060843092002884	27.585093165617604	27.55480836263241	22.799255379747102
44	22.110860928041234	25.90946765815224	28.072274197661777	23.907397216144748
45	23.872892887283417	26.50369033098049	26.407048792232434	23.216367989503656
46	24.858876971926396	27.781920984526593	25.260204103033026	22.09899794051399
47	22.075883700582434	28.327157754510885	27.389034621550408	22.207923923356272
48	21.930561917273618	28.490150382625885	26.75033933823591	22.82894836186459
49	22.61908057605981	25.902893302092483	27.497737051699247	23.980289070148462
50	23.06084219551009	26.63963202916654	26.04175492491512	24.257770850408246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21.0
1	20.0
2	19.0
3	40.0
4	61.0
5	81.0
6	101.0
7	110.0
8	119.0
9	158.5
10	198.0
11	316.0
12	434.0
13	879.0
14	1324.0
15	1884.5
16	2445.0
17	2624.5
18	2804.0
19	2886.5
20	2969.0
21	3510.5
22	4052.0
23	4828.5
24	5605.0
25	7731.5
26	9858.0
27	12991.5
28	16125.0
29	19434.0
30	22743.0
31	26569.0
32	30395.0
33	36853.5
34	43312.0
35	50620.0
36	57928.0
37	67768.0
38	77608.0
39	84287.0
40	90966.0
41	94071.0
42	97176.0
43	93485.0
44	89794.0
45	87305.5
46	84817.0
47	82218.5
48	79620.0
49	76825.5
50	74031.0
51	76980.0
52	79929.0
53	77131.0
54	74333.0
55	71165.0
56	67997.0
57	61176.0
58	54355.0
59	48204.5
60	42054.0
61	35926.5
62	29799.0
63	24033.5
64	18268.0
65	15505.5
66	12743.0
67	9474.5
68	6206.0
69	5050.0
70	3894.0
71	3291.0
72	2688.0
73	2437.0
74	2186.0
75	1741.5
76	1297.0
77	1034.0
78	771.0
79	609.5
80	448.0
81	378.5
82	309.0
83	230.0
84	151.0
85	98.0
86	45.0
87	34.5
88	24.0
89	20.0
90	16.0
91	15.5
92	15.0
93	10.0
94	5.0
95	5.0
96	5.0
97	4.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03254845147386602
2	0.004697714645712623
3	0.0
4	0.0
5	0.0
6	1.6777552306116507E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.001090540899897573
17	0.0
18	1.6777552306116507E-4
19	0.0
20	5.872143307140778E-4
21	0.0
22	0.0
23	0.0
24	0.001090540899897573
25	0.0
26	4.1943880765291276E-4
27	8.388776153058254E-5
28	0.001677755230611651
29	5.872143307140778E-4
30	0.0012583164229587381
31	4.1943880765291276E-4
32	0.001090540899897573
33	5.033265691834953E-4
34	9.227653768364079E-4
35	3.3555104612233014E-4
36	1.6777552306116507E-4
37	0.002013306276733981
38	0.0
39	5.033265691834953E-4
40	0.0
41	0.0014260919460199032
42	0.0049493779303043705
43	0.004362163599590292
44	0.004446051361120874
45	0.002936071653570389
46	0.002013306276733981
47	6.711020922446603E-4
48	0.0036071737458150495
49	0.003355510461223302
50	0.001509979707550486
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1192069.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.12279214112227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.926320096687824	22.988779090139445
2	15.752166875907667	11.695288334131831
3	7.645456521991047	8.514620798695836
4	4.29094098075754	6.3716684047394185
5	2.580919029575887	4.790546033400632
6	1.7028101057740637	3.7927839367471785
7	1.2208609186536992	3.1725236281480593
8	0.8617876547645618	2.559357118208805
9	0.6386907965409858	2.1338987114194947
>10	3.0633422189300767	19.984102281788385
>50	0.2019918193698471	5.236134898850696
>100	0.10857981257325927	7.318574265274062
>500	0.005678859697781819	1.2490374713185222
>1k	4.5430877582254553E-4	0.1926850271376467
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAAAGGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGG	1263	0.10595024281312576	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	8.388776153058254E-5	0.0	0.0	0.0
5	0.0	2.5166328459174763E-4	0.0	0.0	0.0
6	0.0	2.5166328459174763E-4	0.0	0.0	0.0
7	0.0	2.5166328459174763E-4	0.0	0.0	0.0
8	0.0	2.5166328459174763E-4	0.0	0.0	0.0
9	0.0	2.5166328459174763E-4	0.0	0.0	0.0
10	0.0	2.5166328459174763E-4	0.0	0.0	0.0
11	0.0	2.5166328459174763E-4	0.0	0.0	0.0
12	0.0	2.5166328459174763E-4	0.0	0.0	0.0
13	0.0	2.5166328459174763E-4	0.0	0.0	0.0
14	0.0	2.5166328459174763E-4	0.0	0.0	0.0
15	0.0	2.5166328459174763E-4	0.0	0.0	0.0
16	0.0	2.5166328459174763E-4	0.0	0.0	0.0
17	0.0	2.5166328459174763E-4	0.0	0.0	0.0
18	0.0	2.5166328459174763E-4	0.0	0.0	0.0
19	0.0	3.3555104612233014E-4	0.0	0.0	0.0
20	0.0	4.194388076529127E-4	0.0	0.0	0.0
21	0.0	4.194388076529127E-4	0.0	0.0	0.0
22	0.0	4.194388076529127E-4	0.0	0.0	0.0
23	0.0	4.194388076529127E-4	0.0	0.0	0.0
24	0.0	4.194388076529127E-4	0.0	0.0	0.0
25	0.0	4.194388076529127E-4	0.0	0.0	0.0
26	0.0	5.033265691834953E-4	0.0	0.0	0.0
27	0.0	6.711020922446603E-4	0.0	0.0	0.0
28	0.0	6.711020922446603E-4	0.0	8.388776153058254E-5	0.0
29	0.0	6.711020922446603E-4	0.0	3.3555104612233014E-4	0.0
30	0.0	6.711020922446603E-4	0.0	7.549898537752429E-4	0.0
31	0.0	6.711020922446603E-4	0.0	0.002768296130509224	0.0
32	0.0	6.711020922446603E-4	0.0	0.00461382688418204	0.0
33	0.0	6.711020922446603E-4	0.0	0.007885449583874759	0.0
34	0.0	6.711020922446603E-4	0.0	0.011073184522036896	0.0
35	0.0	6.711020922446603E-4	0.0	0.015351460360096604	0.0
36	0.0	6.711020922446603E-4	0.0	0.02139137919029855	0.0
37	0.0	6.711020922446603E-4	0.0	0.029528492058765055	0.0
38	0.0	6.711020922446603E-4	0.0	0.044796064657331075	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1420	0.0	33.318623	1
GTATCAA	3315	0.0	26.619373	1
AGGTCGC	45	0.0013984505	24.4437	6
GCACGAA	215	0.0	22.514719	43
ACGTACG	60	2.8700795E-4	22.00302	43
CGACCCG	140	7.2759576E-12	22.000252	37
TAGGACG	220	0.0	20.99936	4
CGCACGA	160	1.8189894E-12	20.626966	42
GCGTGCA	75	5.4738382E-5	20.532707	35
TACTCGT	55	0.004484368	19.99939	5
TAGGCCC	145	2.9649527E-10	19.723536	4
TGTCGGG	280	0.0	19.642258	23
TGTCACG	80	8.9905414E-5	19.249413	5
CGTCTTA	320	0.0	19.249413	15
GTCACGT	80	8.9905414E-5	19.249413	6
TAGGGTC	355	0.0	19.210682	4
CGCTCCG	185	1.8189894E-12	19.02964	43
TCTCGTC	325	0.0	18.953268	12
GGTCGCG	70	8.124899E-4	18.856567	7
TTGTCGG	280	0.0	18.856567	22
>>END_MODULE