FastQCFastQC Report
Thu 2 Feb 2017
SRR4062292_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062292_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433735
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11570.266752740728786No Hit
CTGTAGGACGTGGAATATGGCAAGA11380.26237218578164084No Hit
GTCCTAAAGTGTGTATTTCTCATTT10910.25153607617554496No Hit
CTTTAGGACGTGAAATATGGCGAGG8600.19827775023920136No Hit
GTCCTACAGTGTGCATTTCTCATTT7470.17222497607986442No Hit
GTATCAACGCAGAGTACTTTTTTTT5720.1318777594614223No Hit
CTGTAGGACCTGGAATATGGCGAGA5470.12611387137307342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAC250.006023300319.001043
CCGTCTG250.006023300319.001049
GTACTAG307.731589E-418.9922641
ATAGGAC701.3023964E-917.6438223
TCCAACG402.7566266E-416.6239918
CCAACGA350.0021676816.28472319
TGAACTG508.6867614E-515.200835
GGTATCA2600.014.6094341
GACGTGA3400.013.4124997
TTGTAGA852.6971793E-713.4063041
GGACGTG5850.013.3169686
TATAGGA957.3443516E-812.9992092
AGGACGT6200.012.8716725
ACGTGAA3650.012.7541238
ACAGTGC604.0795552E-412.6673598
CCTGTAC604.0795552E-412.6673593
GTAAGAC604.0795552E-412.6673593
GTTTAGA751.4789401E-512.6615091
GTATAAA604.09549E-412.6615091
GACGTGG2550.012.294797
Produced by FastQC (version 0.11.5)