##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062292_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	433735
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.314991872917794	32.0	32.0	32.0	32.0	32.0
2	30.950799451277852	32.0	32.0	32.0	32.0	32.0
3	30.984797168778172	32.0	32.0	32.0	32.0	32.0
4	30.978184836363216	32.0	32.0	32.0	32.0	32.0
5	30.960747922118344	32.0	32.0	32.0	32.0	32.0
6	34.60774897114597	36.0	36.0	36.0	32.0	36.0
7	34.516400567166585	36.0	36.0	36.0	32.0	36.0
8	34.499883569460614	36.0	36.0	36.0	32.0	36.0
9	34.57469653129215	36.0	36.0	36.0	32.0	36.0
10	34.35735414481192	36.0	36.0	36.0	32.0	36.0
11	34.56559419922303	36.0	36.0	36.0	32.0	36.0
12	34.43360807866554	36.0	36.0	36.0	32.0	36.0
13	34.48579893252793	36.0	36.0	36.0	32.0	36.0
14	34.435196606222696	36.0	36.0	36.0	32.0	36.0
15	34.412530692704074	36.0	36.0	36.0	32.0	36.0
16	34.410522554094086	36.0	36.0	36.0	32.0	36.0
17	34.37174542059091	36.0	36.0	36.0	32.0	36.0
18	34.37002086527488	36.0	36.0	36.0	32.0	36.0
19	34.34080486933266	36.0	36.0	36.0	32.0	36.0
20	34.32379909391679	36.0	36.0	36.0	32.0	36.0
21	34.30501804096971	36.0	36.0	36.0	32.0	36.0
22	34.26076982489308	36.0	36.0	36.0	32.0	36.0
23	34.24249599409778	36.0	36.0	36.0	32.0	36.0
24	34.217125664288105	36.0	36.0	36.0	32.0	36.0
25	33.8546647146299	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	5.0
5	38.0
6	90.0
7	14.0
8	81.0
9	57.0
10	33.0
11	9.0
12	18.0
13	19.0
14	53.0
15	60.0
16	96.0
17	155.0
18	209.0
19	288.0
20	406.0
21	532.0
22	885.0
23	1239.0
24	1820.0
25	2752.0
26	3919.0
27	5316.0
28	7406.0
29	10239.0
30	13554.0
31	19152.0
32	27621.0
33	40453.0
34	91812.0
35	205404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.70282707834756	21.860172363706788	13.004514287558736	24.432486270386917
2	12.706837124050399	21.93643584216169	44.7835660022825	20.573161031505414
3	17.31714514953935	27.72380179465661	31.007396272352512	23.951656783451533
4	10.322286093759727	18.36641048876714	41.834012400762774	29.47729101671036
5	10.492132975999926	40.97725459082711	37.23055846414288	11.30005396903008
6	27.723343338401236	41.385451969906356	19.575172973858397	11.316031717834015
7	25.52422454005572	33.9524395338585	23.827318070715204	16.696017855370577
8	22.595290522418722	40.45779150336754	21.436616857547765	15.510301116665975
9	25.25178378103871	16.50565893137031	22.674967509908612	35.567589777682365
10	13.719175030187172	30.512477749225987	35.786170927502305	19.982176293084542
11	31.563392541727946	24.916705725889685	26.419350593276885	17.100551139105477
12	21.461667924894204	27.46699172801969	33.5886727200109	17.48266762707521
13	26.87560888897097	24.209194627463837	27.678534326332166	21.236662157233024
14	18.794420373990985	24.78099990997625	29.253795424485073	27.170784291547694
15	20.708180764560026	34.42390185114213	24.430089255618302	20.437828128679545
16	19.5872219311144	31.03152967029923	29.729162001888227	19.652086396698145
17	18.51668024848965	31.815170242187374	29.777665942559278	19.8904835667637
18	18.663890703369702	30.218607109635933	32.571097513404716	18.54640467358965
19	21.822784926979672	29.081660912430994	29.190129793401397	19.905424367187933
20	21.942776436704683	29.81551654904712	30.02113920954126	18.220567804706935
21	21.351643863118714	29.086465792370408	28.121401489365137	21.44048885514574
22	20.91389374219592	31.34372107730337	28.906194102020233	18.836191078480475
23	19.928745148532215	31.177689478372027	29.60583699068241	19.287728382413345
24	20.728862099124694	29.504099618538167	30.57579275060981	19.191245531727333
25	20.15562909322515	30.49882539726495	30.028984303245963	19.316561206263934
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	50.0
2	106.0
3	162.0
4	162.0
5	162.0
6	462.0
7	762.0
8	762.0
9	762.0
10	1004.5
11	1247.0
12	1247.0
13	1247.0
14	2114.5
15	2982.0
16	2982.0
17	2982.0
18	5226.5
19	7471.0
20	7471.0
21	7471.0
22	11761.0
23	16051.0
24	16051.0
25	16051.0
26	23310.0
27	30569.0
28	30569.0
29	30569.0
30	38882.0
31	47195.0
32	47195.0
33	47195.0
34	52605.5
35	58016.0
36	58016.0
37	58016.0
38	60289.5
39	62563.0
40	62563.0
41	62563.0
42	61542.0
43	60521.0
44	60521.0
45	60521.0
46	58515.0
47	56509.0
48	56509.0
49	56509.0
50	49163.5
51	41818.0
52	41818.0
53	41818.0
54	33160.5
55	24503.0
56	24503.0
57	24503.0
58	18747.5
59	12992.0
60	12992.0
61	12992.0
62	9556.5
63	6121.0
64	6121.0
65	6121.0
66	4290.0
67	2459.0
68	2459.0
69	2459.0
70	1725.0
71	991.0
72	991.0
73	991.0
74	691.0
75	391.0
76	391.0
77	391.0
78	266.0
79	141.0
80	141.0
81	141.0
82	85.0
83	29.0
84	29.0
85	29.0
86	21.5
87	14.0
88	14.0
89	14.0
90	10.5
91	7.0
92	7.0
93	7.0
94	8.5
95	10.0
96	10.0
97	10.0
98	85.5
99	161.0
100	161.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.3055552353395506E-4
2	0.0
3	6.916665706018652E-4
4	0.011988887223765662
5	0.03527499510069513
6	0.06639999077777906
7	0.1097444292021626
8	0.1108972068198324
9	0.12127220537886037
10	0.13925553621450887
11	0.1457110908734596
12	0.13510553679089765
13	0.13118609289082042
14	0.1189666501435208
15	0.13764164754977118
16	0.12081109433179245
17	0.12864998213194692
18	0.1134333175787059
19	0.09936943064313462
20	0.09614165331365926
21	0.0912999873194462
22	0.10789998501389098
23	0.08323054399575779
24	0.09268332046064993
25	0.09153054284298015
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	433735.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.37241990359317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.42928969657147	35.66750623900484
2	16.245040269873286	16.690940601894216
3	6.228539940864609	9.59925507685194
4	2.95583692147264	6.073939819857464
5	1.5889388025292335	4.081381568232214
6	0.9218153742159374	2.8413531886685406
7	0.5759711965735247	2.0712323913925075
8	0.4120691995988639	1.6935193560904303
9	0.2777593579861609	1.2842253333555762
>10	1.227768081754334	11.123662231928027
>50	0.08245303294995503	3.0046488184786244
>100	0.051364184458998596	4.453620515751977
>500	0.0018022520862806528	0.6309739372142763
>1k	0.0013516890647104895	0.7837409212793615
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	1157	0.266752740728786	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1138	0.26237218578164084	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1091	0.25153607617554496	No Hit
CTTTAGGACGTGAAATATGGCGAGG	860	0.19827775023920136	No Hit
GTCCTACAGTGTGCATTTCTCATTT	747	0.17222497607986442	No Hit
GTATCAACGCAGAGTACTTTTTTTT	572	0.1318777594614223	No Hit
CTGTAGGACCTGGAATATGGCGAGA	547	0.12611387137307342	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.3055552353395506E-4	0.0
13	0.0	0.0	0.0	2.3055552353395506E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTGAC	25	0.0060233003	19.00104	3
CCGTCTG	25	0.0060233003	19.00104	9
GTACTAG	30	7.731589E-4	18.992264	1
ATAGGAC	70	1.3023964E-9	17.643822	3
TCCAACG	40	2.7566266E-4	16.62399	18
CCAACGA	35	0.00216768	16.284723	19
TGAACTG	50	8.6867614E-5	15.20083	5
GGTATCA	260	0.0	14.609434	1
GACGTGA	340	0.0	13.412499	7
TTGTAGA	85	2.6971793E-7	13.406304	1
GGACGTG	585	0.0	13.316968	6
TATAGGA	95	7.3443516E-8	12.999209	2
AGGACGT	620	0.0	12.871672	5
ACGTGAA	365	0.0	12.754123	8
ACAGTGC	60	4.0795552E-4	12.667359	8
CCTGTAC	60	4.0795552E-4	12.667359	3
GTAAGAC	60	4.0795552E-4	12.667359	3
GTTTAGA	75	1.4789401E-5	12.661509	1
GTATAAA	60	4.09549E-4	12.661509	1
GACGTGG	255	0.0	12.29479	7
>>END_MODULE