FastQCFastQC Report
Thu 2 Feb 2017
SRR4062292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433735
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA4830.1113583178669003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT353.212709E-431.43153644
CGTTTTG353.2148825E-431.42791225
CGCGGTA300.005738357529.33610243
GGCCGCG300.005738357529.33610240
TAGGACC2500.029.039394
GGTATCA3250.025.7255061
GTATCAA7300.025.6188561
TGACCGT450.001396940724.4439325
GCGGGGT651.808595E-523.69454244
CTGTAGG7250.023.3677221
GACTCAC952.6759153E-823.1574087
GTCCTAC6850.022.805071
GACCGTG500.002578109321.9995386
TTCGTAC500.002578109321.99953824
CGTACAT500.002578109321.99953826
TACACCG500.002578109321.9995385
TGTAGGA8100.021.727942
AGGACCT3650.021.0954485
TCTAGAC755.463152E-520.5329043
GTAGGAC8150.020.5149063
Produced by FastQC (version 0.11.5)