##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062292_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	433735
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25481227016496	32.0	32.0	32.0	32.0	32.0
2	31.44349429951468	32.0	32.0	32.0	32.0	32.0
3	31.588154057200825	32.0	32.0	32.0	32.0	32.0
4	31.655430158968034	32.0	32.0	32.0	32.0	32.0
5	31.64216399414389	32.0	32.0	32.0	32.0	32.0
6	35.26416129664427	36.0	36.0	36.0	36.0	36.0
7	35.268643296021764	36.0	36.0	36.0	36.0	36.0
8	35.216420164386086	36.0	36.0	36.0	36.0	36.0
9	35.323450955076254	36.0	36.0	36.0	36.0	36.0
10	35.20692127681649	36.0	36.0	36.0	36.0	36.0
11	35.31934245564688	36.0	36.0	36.0	36.0	36.0
12	35.252898659319634	36.0	36.0	36.0	36.0	36.0
13	35.28171579420614	36.0	36.0	36.0	36.0	36.0
14	35.26291629681718	36.0	36.0	36.0	36.0	36.0
15	35.24021579997003	36.0	36.0	36.0	36.0	36.0
16	35.26024185274419	36.0	36.0	36.0	36.0	36.0
17	35.25454943686813	36.0	36.0	36.0	36.0	36.0
18	35.251194854000715	36.0	36.0	36.0	36.0	36.0
19	35.22843441271744	36.0	36.0	36.0	36.0	36.0
20	35.22329533009787	36.0	36.0	36.0	36.0	36.0
21	35.211940470563825	36.0	36.0	36.0	36.0	36.0
22	35.17179844836133	36.0	36.0	36.0	36.0	36.0
23	35.16638961577922	36.0	36.0	36.0	36.0	36.0
24	35.1614949220146	36.0	36.0	36.0	36.0	36.0
25	35.12845631549218	36.0	36.0	36.0	36.0	36.0
26	35.10111012484582	36.0	36.0	36.0	36.0	36.0
27	35.068557990478055	36.0	36.0	36.0	36.0	36.0
28	35.02597899639181	36.0	36.0	36.0	36.0	36.0
29	35.004820915997094	36.0	36.0	36.0	36.0	36.0
30	34.96012772776004	36.0	36.0	36.0	36.0	36.0
31	34.95719736705592	36.0	36.0	36.0	36.0	36.0
32	34.935884814460444	36.0	36.0	36.0	36.0	36.0
33	34.95154875672934	36.0	36.0	36.0	36.0	36.0
34	34.93528767565449	36.0	36.0	36.0	36.0	36.0
35	34.908446401604664	36.0	36.0	36.0	36.0	36.0
36	34.87644990604863	36.0	36.0	36.0	36.0	36.0
37	34.81469560907006	36.0	36.0	36.0	32.0	36.0
38	34.81711183095669	36.0	36.0	36.0	32.0	36.0
39	34.706514346317455	36.0	36.0	36.0	32.0	36.0
40	34.76669856018076	36.0	36.0	36.0	32.0	36.0
41	34.73355850922798	36.0	36.0	36.0	32.0	36.0
42	34.723912066123326	36.0	36.0	36.0	32.0	36.0
43	34.74508628540468	36.0	36.0	36.0	32.0	36.0
44	34.674056739714345	36.0	36.0	36.0	32.0	36.0
45	34.66242751910729	36.0	36.0	36.0	32.0	36.0
46	34.56203211638443	36.0	36.0	36.0	32.0	36.0
47	34.56636425467163	36.0	36.0	36.0	32.0	36.0
48	34.55498172847476	36.0	36.0	36.0	32.0	36.0
49	34.50791842945577	36.0	36.0	36.0	32.0	36.0
50	34.144733535453675	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	5.0
22	7.0
23	48.0
24	141.0
25	399.0
26	918.0
27	1768.0
28	3331.0
29	5447.0
30	8196.0
31	12199.0
32	18889.0
33	31882.0
34	71724.0
35	278778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.423997195630164	22.666734315939014	13.276938439930905	24.632330048499913
2	12.469911186121795	22.58228887105848	44.08460836123177	20.86319158158795
3	16.77364467623013	28.043685761319146	31.271701088685695	23.910968473765028
4	10.202797106060396	18.928191011080525	41.86091014308309	29.008101739775995
5	10.256031908884458	41.28949704312541	37.10053373603699	11.353937311953151
6	27.745346226027934	41.70851259066617	19.3519530403427	11.194188142963199
7	25.36894647653521	34.11945081674294	23.85442724244066	16.657175464281185
8	22.663838518911316	40.669072129295536	21.276816489331043	15.390272862462103
9	25.34335481342294	16.400106055540824	22.560549644368106	35.695989486668125
10	13.814195303583984	30.47298465652991	35.60538116591928	20.107438873966824
11	31.89528168121088	24.681429905356957	26.361257449825352	17.062030963606812
12	21.40846369326893	27.640379494391738	33.54352311895512	17.40763369338421
13	27.08566290476904	23.965785560307562	27.81375724808927	21.134794286834126
14	18.76122517205206	24.883165988449168	29.351101479013685	27.004507360485093
15	20.82492766320449	34.46643687966154	24.374099392486194	20.334536064647768
16	19.736195937113095	30.96089511701954	29.580085352441955	19.722823593425417
17	18.510380762447117	32.01655388658974	29.69877920850289	19.774286142460255
18	18.908824302452658	30.24641831168412	32.298828314128016	18.545929071735213
19	21.700346986062918	29.22798482944655	29.14936539592147	19.922302788569056
20	22.102307179762665	29.97304793501993	29.63574364874243	18.288901236474974
21	21.525585899224183	29.02209874693073	28.110712762401008	21.341602591444083
22	20.97478875350156	31.28730676565184	28.814829331273707	18.923075149572895
23	19.909391679251158	31.320276205517196	29.500962569310758	19.2693695459209
24	20.885064706004414	29.55956572161086	30.445598769744382	19.109770802640348
25	20.353535469978073	30.36960526406799	29.839094558172885	19.437764707781056
26	18.748717523073058	31.15387186989171	30.725726314236248	19.371684292798992
27	19.253275048762607	31.286226120156595	29.685705985696302	19.774792845384496
28	18.989133717753333	31.09813315749308	29.1141662843217	20.798566840431885
29	20.03532118764035	30.024623386684002	29.09709637704215	20.842959048633496
30	19.771792985942835	29.95856859928665	30.028658448017083	20.240979966753432
31	21.00849829619075	29.219752198351983	29.723978290841853	20.04777121461541
32	19.041437935319657	30.76016812186299	30.261935338099704	19.93645860471765
33	19.226761102426835	31.23965379702767	30.337718509501215	19.19586659104428
34	19.443618279532707	30.664859094692332	31.1707025783262	18.720820047448765
35	19.525747288090656	31.166495671320043	30.043229160662616	19.264527879926682
36	18.95954663457326	30.231893280213217	31.117920199938208	19.690639885275306
37	19.438264161557846	30.032485872449723	29.306914257124873	21.22233570886756
38	19.66062226935802	31.4459289658432	29.341879258072325	19.55156950672646
39	20.448063670506972	30.08970956786779	29.44023166325827	20.02199509836697
40	21.19727483371183	29.54315423011747	30.222140246924965	19.03743068924574
41	21.22716331744026	28.993940958933166	30.712744275266758	19.06615144835982
42	19.667708823285285	31.4802774165583	29.951396766547695	18.900616993608722
43	18.905689437327652	31.41018546698761	30.24029549290319	19.44382960278154
44	18.76609647393553	31.0057572634438	30.31659184760429	19.911554415016383
45	19.29909504870598	31.128941149345785	29.84033661882529	19.731627183122946
46	20.0874288718171	29.83763868266455	29.70091578976492	20.37401665575343
47	20.97890166946794	29.958430455743308	29.39540867496213	19.66725919982662
48	19.43460697783803	30.5384168442022	29.426398841638306	20.600577336321464
49	20.38568572884412	29.848912088646852	29.6736842347961	20.09171794771293
50	19.281527969270922	31.385944121403835	29.15458146387351	20.177946445451735
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13.0
1	7.5
2	2.0
3	6.5
4	11.0
5	24.5
6	38.0
7	34.0
8	30.0
9	53.0
10	76.0
11	121.5
12	167.0
13	262.0
14	357.0
15	514.0
16	671.0
17	937.0
18	1203.0
19	1496.5
20	1790.0
21	2521.5
22	3253.0
23	4049.5
24	4846.0
25	6566.0
26	8286.0
27	11002.0
28	13718.0
29	16510.5
30	19303.0
31	21666.5
32	24030.0
33	28381.5
34	32733.0
35	36746.5
36	40760.0
37	42792.5
38	44825.0
39	44035.0
40	43245.0
41	41236.0
42	39227.0
43	36530.5
44	33834.0
45	31501.5
46	29169.0
47	26749.5
48	24330.0
49	21480.5
50	18631.0
51	16896.5
52	15162.0
53	13181.5
54	11201.0
55	9434.0
56	7667.0
57	6749.5
58	5832.0
59	4701.5
60	3571.0
61	2988.0
62	2405.0
63	1863.5
64	1322.0
65	1130.0
66	938.0
67	713.0
68	488.0
69	375.5
70	263.0
71	197.0
72	131.0
73	105.0
74	79.0
75	70.5
76	62.0
77	51.0
78	40.0
79	29.5
80	19.0
81	11.0
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	1.0
88	2.0
89	1.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02904999596527834
2	0.004380554947145146
3	2.3055552353395506E-4
4	2.3055552353395506E-4
5	0.0
6	2.3055552353395506E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.222220941358202E-4
17	0.0
18	2.3055552353395506E-4
19	0.0
20	4.611110470679101E-4
21	0.0
22	0.0
23	0.0
24	9.222220941358202E-4
25	4.611110470679101E-4
26	9.222220941358202E-4
27	2.3055552353395506E-4
28	0.0018444441882716405
29	2.3055552353395506E-4
30	0.0013833331412037304
31	2.3055552353395506E-4
32	4.611110470679101E-4
33	2.3055552353395506E-4
34	9.222220941358202E-4
35	0.0
36	2.3055552353395506E-4
37	0.0018444441882716405
38	0.0
39	4.611110470679101E-4
40	0.0
41	6.916665706018652E-4
42	0.004380554947145146
43	0.004380554947145146
44	0.005072221517747011
45	0.0023055552353395507
46	0.0025361107588735055
47	9.222220941358202E-4
48	0.004380554947145146
49	0.003227777329475371
50	0.0020749997118055955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	433735.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.85921314699298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.00858632643207	39.10624545727827
2	16.268449231323448	18.17485546367061
3	6.303284016632804	10.562894562333774
4	2.8555937676900296	6.380448837224885
5	1.50380440966098	4.200066552532031
6	0.868664203502382	2.911373933796147
7	0.5079607721244175	1.9862002328286281
8	0.34542931071264	1.5436327595452979
9	0.2636973735905582	1.3256935017927505
>10	0.9965642635497677	9.407583207330934
>50	0.053912884164264714	2.091392651104687
>100	0.024053440616666682	2.3096128405619787
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	483	0.1113583178669003	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.3055552353395506E-4	0.0
13	0.0	0.0	0.0	2.3055552353395506E-4	0.0
14	0.0	0.0	0.0	2.3055552353395506E-4	0.0
15	0.0	0.0	0.0	2.3055552353395506E-4	0.0
16	0.0	0.0	0.0	2.3055552353395506E-4	0.0
17	0.0	0.0	0.0	2.3055552353395506E-4	0.0
18	0.0	0.0	0.0	4.611110470679101E-4	0.0
19	0.0	0.0	0.0	4.611110470679101E-4	0.0
20	0.0	0.0	0.0	6.916665706018652E-4	0.0
21	0.0	0.0	0.0	9.222220941358202E-4	0.0
22	0.0	0.0	0.0	0.0013833331412037304	0.0
23	0.0	0.0	0.0	0.0013833331412037304	0.0
24	0.0	0.0	0.0	0.0016138886647376854	0.0
25	0.0	0.0	0.0	0.0016138886647376854	0.0
26	0.0	0.0	0.0	0.0016138886647376854	0.0
27	0.0	0.0	0.0	0.0020749997118055955	0.0
28	0.0	0.0	0.0	0.002766666282407461	0.0
29	0.0	0.0	0.0	0.0046111104706791015	0.0
30	0.0	0.0	0.0	0.008530554370756338	0.0
31	0.0	0.0	0.0	0.014294442459105215	0.0
32	0.0	0.0	0.0	0.021211108165123865	0.0
33	0.0	0.0	0.0	0.02904999596527834	0.0
34	0.0	0.0	0.0	0.037349994812500724	0.0
35	0.0	0.0	0.0	0.04657221575385892	0.0
36	0.0	0.0	0.0	0.057869436407022724	0.0
37	0.0	0.0	0.0	0.08392221056635964	0.0
38	0.0	0.0	0.0	0.10859165158449284	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGTAT	35	3.212709E-4	31.431536	44
CGTTTTG	35	3.2148825E-4	31.427912	25
CGCGGTA	30	0.0057383575	29.336102	43
GGCCGCG	30	0.0057383575	29.336102	40
TAGGACC	250	0.0	29.03939	4
GGTATCA	325	0.0	25.725506	1
GTATCAA	730	0.0	25.618856	1
TGACCGT	45	0.0013969407	24.443932	5
GCGGGGT	65	1.808595E-5	23.694542	44
CTGTAGG	725	0.0	23.367722	1
GACTCAC	95	2.6759153E-8	23.157408	7
GTCCTAC	685	0.0	22.80507	1
GACCGTG	50	0.0025781093	21.999538	6
TTCGTAC	50	0.0025781093	21.999538	24
CGTACAT	50	0.0025781093	21.999538	26
TACACCG	50	0.0025781093	21.999538	5
TGTAGGA	810	0.0	21.72794	2
AGGACCT	365	0.0	21.095448	5
TCTAGAC	75	5.463152E-5	20.532904	3
GTAGGAC	815	0.0	20.514906	3
>>END_MODULE