FastQCFastQC Report
Thu 2 Feb 2017
SRR4062291_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062291_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4192422
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81890.1953286191132477No Hit
TATCAACGCAGAGTACTTTTTTTTT46540.11100981723690982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26150.013.908731
GTATCAA63750.011.8723991
CCGTCCG752.0720725E-411.40197859
GTCCTAA2950.010.9450381
AAGACGG7800.010.7194255
CGAACGA2400.010.68667316
GCGTTAT2650.010.3923191
CGGTCCA4850.010.38299710
CAAGACG7900.010.3425784
CGTCTTA1951.4551915E-1110.23034515
GACGGAC8300.010.1883937
CGGACCA8150.010.1428639
TAACGAA2650.010.03827813
CGCAAGA8200.09.9613352
CGACTTT3350.09.9259113
GCGCAAG8200.09.728051
GTATTAG3250.09.6425291
ACGGACC9000.09.6069938
CGTCGTA6750.09.57180310
AACCGCG1901.0513759E-99.5015347
Produced by FastQC (version 0.11.5)