##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062291_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4192422
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.230883007483502	32.0	32.0	32.0	32.0	32.0
2	30.838649353524048	32.0	32.0	32.0	32.0	32.0
3	30.860057265227596	32.0	32.0	32.0	32.0	32.0
4	30.853435078816016	32.0	32.0	32.0	32.0	32.0
5	30.805595190560492	32.0	32.0	32.0	32.0	32.0
6	34.444801358260214	36.0	36.0	36.0	32.0	36.0
7	34.37432777520965	36.0	36.0	36.0	32.0	36.0
8	34.35555533293166	36.0	36.0	36.0	32.0	36.0
9	34.44960454839708	36.0	36.0	36.0	32.0	36.0
10	34.18632380041895	36.0	36.0	36.0	32.0	36.0
11	34.4241095004272	36.0	36.0	36.0	32.0	36.0
12	34.282581047423186	36.0	36.0	36.0	32.0	36.0
13	34.347668722280346	36.0	36.0	36.0	32.0	36.0
14	34.271521330629405	36.0	36.0	36.0	32.0	36.0
15	34.236128901145925	36.0	36.0	36.0	32.0	36.0
16	34.2356389695503	36.0	36.0	36.0	32.0	36.0
17	34.17540696046343	36.0	36.0	36.0	32.0	36.0
18	34.17242181249884	36.0	36.0	36.0	32.0	36.0
19	34.177306339867506	36.0	36.0	36.0	32.0	36.0
20	34.15661662876495	36.0	36.0	36.0	32.0	36.0
21	34.139119344378976	36.0	36.0	36.0	32.0	36.0
22	34.11369943197512	36.0	36.0	36.0	32.0	36.0
23	34.09535299643023	36.0	36.0	36.0	32.0	36.0
24	34.07008764861934	36.0	36.0	36.0	32.0	36.0
25	33.71987648189996	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	53.0
5	344.0
6	853.0
7	172.0
8	694.0
9	588.0
10	320.0
11	83.0
12	138.0
13	193.0
14	687.0
15	896.0
16	1470.0
17	2099.0
18	3038.0
19	4066.0
20	6086.0
21	8748.0
22	13013.0
23	18798.0
24	26087.0
25	36204.0
26	48186.0
27	61210.0
28	79524.0
29	104330.0
30	135852.0
31	182432.0
32	258198.0
33	369920.0
34	839447.0
35	1988688.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.26241590999677	17.864135981850897	12.546229674149522	25.32721843400281
2	16.6863291650109	19.802218960245817	36.968438708581736	26.54301316616155
3	19.55868227236959	22.807132622634438	28.15464367776063	29.47954142723534
4	13.657545955222389	16.10728248664527	34.362080181566576	35.87309137656576
5	15.601311482920108	35.75086596670326	32.32939972765326	16.318422822723367
6	34.358377667509195	34.11688469772804	16.802521379455197	14.722216255307572
7	30.909736048225845	30.437047900625654	19.393800612326473	19.25941543882203
8	28.51645710593433	32.535691626827564	18.795946499989135	20.151904767248972
9	26.967125539641017	15.66147009699807	18.942635544880414	38.4287688184805
10	17.52806187094107	26.681258178766242	29.55418902106542	26.236490929227262
11	36.30060879601321	21.961943561208265	21.675292550752108	20.062155092026416
12	25.09574421587906	23.78072562352861	27.28104100921509	23.842489151377247
13	28.990851455202797	19.92556209360433	24.88131443280503	26.202272018387845
14	24.041420785279403	19.956605795700508	24.623297615658977	31.378675803361116
15	25.369669377253	26.708052727102068	22.112786090576908	25.809491805068024
16	25.865872559110308	26.15167486167197	23.153496946872963	24.82895563234476
17	24.17979277643044	26.289141002539846	24.451879687576497	25.079186533453218
18	24.651728106747665	25.52533298947901	25.372628240946366	24.45031066282696
19	25.611425905592217	25.143806284286274	25.099947546190748	24.14482026393076
20	26.0428922036747	24.44189522136063	24.688514649146263	24.826697925818404
21	26.698051450418202	24.263355244906837	24.428635069881636	24.609958234793325
22	26.19201224770857	24.271097069584826	24.706390220934374	24.830500461772232
23	24.74674112033321	24.42361628161514	25.186478314040773	25.643164284010883
24	24.635635229125977	24.958894291660368	25.183313434040226	25.222157045173425
25	24.932068039391226	24.648642196359297	25.04062071023575	25.378669054013724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	655.0
1	655.0
2	1095.0
3	1535.0
4	1535.0
5	1535.0
6	2690.5
7	3846.0
8	3846.0
9	3846.0
10	4746.0
11	5646.0
12	5646.0
13	5646.0
14	7647.0
15	9648.0
16	9648.0
17	9648.0
18	16189.5
19	22731.0
20	22731.0
21	22731.0
22	36200.0
23	49669.0
24	49669.0
25	49669.0
26	73991.0
27	98313.0
28	98313.0
29	98313.0
30	127730.5
31	157148.0
32	157148.0
33	157148.0
34	200980.5
35	244813.0
36	244813.0
37	244813.0
38	293393.0
39	341973.0
40	341973.0
41	341973.0
42	395733.5
43	449494.0
44	449494.0
45	449494.0
46	501549.0
47	553604.0
48	553604.0
49	553604.0
50	573998.0
51	594392.0
52	594392.0
53	594392.0
54	557700.0
55	521008.0
56	521008.0
57	521008.0
58	475828.5
59	430649.0
60	430649.0
61	430649.0
62	373728.5
63	316808.0
64	316808.0
65	316808.0
66	255014.5
67	193221.0
68	193221.0
69	193221.0
70	147185.0
71	101149.0
72	101149.0
73	101149.0
74	74700.0
75	48251.0
76	48251.0
77	48251.0
78	38128.5
79	28006.0
80	28006.0
81	28006.0
82	19417.5
83	10829.0
84	10829.0
85	10829.0
86	7942.0
87	5055.0
88	5055.0
89	5055.0
90	3431.5
91	1808.0
92	1808.0
93	1808.0
94	1153.0
95	498.0
96	498.0
97	498.0
98	1085.5
99	1673.0
100	1673.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.302498651137695E-4
2	1.9082048515154247E-4
3	5.486088948106847E-4
4	0.010996030456857635
5	0.03437153988792159
6	0.06325699082773632
7	0.10638242047198493
8	0.10817136252028065
9	0.11725918812562286
10	0.13149916683005672
11	0.1382971466135804
12	0.13083129513202632
13	0.12660939189804843
14	0.11368130402903143
15	0.13130834634490518
16	0.11716377788304708
17	0.12300765524081306
18	0.10976948408342481
19	0.09481392855967267
20	0.0902103843553917
21	0.0898764485063765
22	0.10041928031099923
23	0.0794051743836856
24	0.0913791598269449
25	0.08758660268455801
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4192422.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.40422603913839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.48450190673314	33.17182984775264
2	15.592350618096734	14.471019251273201
3	5.448498284584489	7.585000379151492
4	2.377458714980112	4.412965264346265
5	1.2965324439044354	3.0082292297008975
6	0.7995698424915723	2.226205182303432
7	0.5342657473923085	1.7354531954873327
8	0.36164724032644446	1.3425568229231148
9	0.27805498808523377	1.1612633866575408
>10	1.637655116212721	13.848953549797564
>50	0.10764403660949327	3.4508729301866645
>100	0.06687545488665529	6.159285398052583
>500	0.00961897127131378	3.0392618597463144
>1k	0.005274919528060035	4.190584917088511
>5k	5.171489733392161E-5	0.19651878553247687
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8189	0.1953286191132477	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4654	0.11100981723690982	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6696792450759967E-4
2	0.0	0.0	0.0	0.0	1.6696792450759967E-4
3	0.0	0.0	0.0	2.385256064394281E-5	1.6696792450759967E-4
4	0.0	0.0	0.0	2.385256064394281E-5	1.9082048515154247E-4
5	0.0	0.0	0.0	2.385256064394281E-5	2.862307277273137E-4
6	2.385256064394281E-5	0.0	0.0	2.385256064394281E-5	3.100832883712565E-4
7	2.385256064394281E-5	0.0	0.0	2.385256064394281E-5	3.100832883712565E-4
8	2.385256064394281E-5	0.0	0.0	2.385256064394281E-5	3.100832883712565E-4
9	2.385256064394281E-5	0.0	0.0	2.385256064394281E-5	3.100832883712565E-4
10	2.385256064394281E-5	0.0	0.0	7.155768193182842E-5	3.3393584901519934E-4
11	2.385256064394281E-5	0.0	0.0	7.155768193182842E-5	3.5778840965914216E-4
12	2.385256064394281E-5	0.0	0.0	7.155768193182842E-5	8.825447438258839E-4
13	2.385256064394281E-5	0.0	0.0	7.155768193182842E-5	0.0011687754715531977
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2615	0.0	13.90873	1
GTATCAA	6375	0.0	11.872399	1
CCGTCCG	75	2.0720725E-4	11.4019785	9
GTCCTAA	295	0.0	10.945038	1
AAGACGG	780	0.0	10.719425	5
CGAACGA	240	0.0	10.686673	16
GCGTTAT	265	0.0	10.392319	1
CGGTCCA	485	0.0	10.382997	10
CAAGACG	790	0.0	10.342578	4
CGTCTTA	195	1.4551915E-11	10.230345	15
GACGGAC	830	0.0	10.188393	7
CGGACCA	815	0.0	10.142863	9
TAACGAA	265	0.0	10.038278	13
CGCAAGA	820	0.0	9.961335	2
CGACTTT	335	0.0	9.92591	13
GCGCAAG	820	0.0	9.72805	1
GTATTAG	325	0.0	9.642529	1
ACGGACC	900	0.0	9.606993	8
CGTCGTA	675	0.0	9.571803	10
AACCGCG	190	1.0513759E-9	9.501534	7
>>END_MODULE