FastQCFastQC Report
Thu 2 Feb 2017
SRR4062291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4192422
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30550.027.5158841
GTATCAA67050.025.7633441
TCAACGC100300.017.064194
ATCAACG101100.016.9509223
TATCAAC102600.016.8535982
ACCGTCG10050.016.4173188
CAACGCA104800.016.3524655
AACGCAG105300.016.295716
TAGGACG4050.016.295714
ATACCGT11250.016.2305266
GTATTAG6950.016.1479241
TACCGTC10550.015.6392467
CGTCGTA11000.015.19963410
CCGTCGT11100.014.8643299
CGAGCCG12900.014.32523615
GTATACG1901.7507409E-713.8982131
ACGCAGA125700.013.6510597
CGCAGAG129550.013.3132998
TTACCGC11950.013.254909516
AGAGTAC132250.013.10819111
Produced by FastQC (version 0.11.5)