##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062291_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4192422
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.296281481205853	32.0	32.0	32.0	32.0	32.0
2	31.472229417744682	32.0	32.0	32.0	32.0	32.0
3	31.56967237553853	32.0	32.0	32.0	32.0	32.0
4	31.63893353293156	32.0	32.0	32.0	32.0	32.0
5	31.61855223543813	32.0	32.0	32.0	32.0	32.0
6	35.24671204377803	36.0	36.0	36.0	36.0	36.0
7	35.24713161031976	36.0	36.0	36.0	36.0	36.0
8	35.206387143278995	36.0	36.0	36.0	36.0	36.0
9	35.27677843499533	36.0	36.0	36.0	36.0	36.0
10	35.16410943364003	36.0	36.0	36.0	36.0	36.0
11	35.27869403414065	36.0	36.0	36.0	36.0	36.0
12	35.202283071694595	36.0	36.0	36.0	36.0	36.0
13	35.237894944736006	36.0	36.0	36.0	36.0	36.0
14	35.19640914011042	36.0	36.0	36.0	36.0	36.0
15	35.178518526999426	36.0	36.0	36.0	36.0	36.0
16	35.19312798186824	36.0	36.0	36.0	36.0	36.0
17	35.173881827735855	36.0	36.0	36.0	36.0	36.0
18	35.16937059294126	36.0	36.0	36.0	36.0	36.0
19	35.1648233407801	36.0	36.0	36.0	36.0	36.0
20	35.16105582882639	36.0	36.0	36.0	36.0	36.0
21	35.14879418150176	36.0	36.0	36.0	36.0	36.0
22	35.13593502753301	36.0	36.0	36.0	36.0	36.0
23	35.10686853565791	36.0	36.0	36.0	36.0	36.0
24	35.09117975241996	36.0	36.0	36.0	36.0	36.0
25	35.076112089861184	36.0	36.0	36.0	36.0	36.0
26	35.03385155406588	36.0	36.0	36.0	36.0	36.0
27	35.02178335100808	36.0	36.0	36.0	36.0	36.0
28	34.99947238135856	36.0	36.0	36.0	36.0	36.0
29	34.98111807446865	36.0	36.0	36.0	36.0	36.0
30	34.96317260046818	36.0	36.0	36.0	36.0	36.0
31	34.961065465260894	36.0	36.0	36.0	36.0	36.0
32	34.93704784489729	36.0	36.0	36.0	36.0	36.0
33	34.914014381185865	36.0	36.0	36.0	32.0	36.0
34	34.904949215513135	36.0	36.0	36.0	32.0	36.0
35	34.896165510056	36.0	36.0	36.0	32.0	36.0
36	34.87134739775719	36.0	36.0	36.0	32.0	36.0
37	34.86307342152102	36.0	36.0	36.0	32.0	36.0
38	34.84723651388148	36.0	36.0	36.0	32.0	36.0
39	34.83295717845198	36.0	36.0	36.0	32.0	36.0
40	34.82359791070651	36.0	36.0	36.0	32.0	36.0
41	34.798415331281056	36.0	36.0	36.0	32.0	36.0
42	34.76638921368126	36.0	36.0	36.0	32.0	36.0
43	34.757368413771324	36.0	36.0	36.0	32.0	36.0
44	34.716824308239964	36.0	36.0	36.0	32.0	36.0
45	34.691087395305146	36.0	36.0	36.0	32.0	36.0
46	34.67982683995075	36.0	36.0	36.0	32.0	36.0
47	34.65138385401088	36.0	36.0	36.0	32.0	36.0
48	34.62571158151541	36.0	36.0	36.0	32.0	36.0
49	34.61008171410226	36.0	36.0	36.0	32.0	36.0
50	34.20486701958915	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	10.0
20	43.0
21	150.0
22	419.0
23	967.0
24	2482.0
25	5606.0
26	11539.0
27	21163.0
28	35880.0
29	55393.0
30	80651.0
31	116869.0
32	173731.0
33	287967.0
34	653894.0
35	2745655.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.90042538083372	18.330482826811686	13.033026426676924	25.736065365677675
2	16.206881746079073	20.215731409147832	36.677249686922295	26.900137157850796
3	18.913232328677463	23.16090443357932	28.45043452292641	29.475428714816807
4	13.416100207565181	16.433237334636004	34.45455581957715	35.696106638221664
5	15.225454510759896	35.944537999532486	32.2636806426837	16.566326847023916
6	34.43470304473602	34.28524268584034	16.686893667040675	14.593160602382971
7	30.969282195351518	30.34911561860901	19.438238803250247	19.243363382789234
8	28.57722815117371	32.482417084921316	18.79968190225125	20.140672861653716
9	27.167064765903813	15.32946826440659	18.858072970707624	38.64539399898197
10	17.552400547846137	26.53101384451646	29.725017877511263	26.19156773012615
11	36.5920701685088	21.782993219671113	21.65709940459238	19.967837207227706
12	25.231405617087212	23.63900866849759	27.270179385567577	23.85940632884762
13	29.20450279098812	19.741834195126348	24.890027769151104	26.16363524473443
14	24.121379002400044	19.653603573304405	24.734389810949374	31.490627613346177
15	25.466758832960995	26.565383923660356	22.117573087823697	25.850284155554952
16	25.97948423152931	26.00059393400177	23.16686086797849	24.853060966490435
17	24.321215755475	26.15137502856344	24.453931402897897	25.073477813063665
18	24.926331296163692	25.242473436570496	25.443145220729424	24.388050046536392
19	25.750461189260047	24.935299929253304	25.231906520860736	24.08233236062591
20	26.24573932415961	24.222893276182432	24.724156272883615	24.807211126774337
21	26.91409734711694	24.052027230096222	24.475839729798064	24.558035692988774
22	26.294013341214217	24.087484513725002	24.77961426593029	24.83888787913049
23	24.90080903192382	24.17597672751143	25.27324326915875	25.649970971406
24	24.791645994260065	24.777525150367904	25.23366180197234	25.19716705339969
25	25.208817441390625	24.282597775317253	25.20113690953526	25.30744787375686
26	24.789643364523076	25.111439641790245	25.452485018142333	24.646431975544342
27	25.352476176596234	24.646510086916575	24.844844649057546	25.156169087429642
28	25.029684964881337	24.55632367273978	25.284267230995063	25.129724131383817
29	24.90183441202975	24.732504437785842	25.29425823739399	25.071402912790415
30	24.93878759240897	24.683538114130698	25.424384160840802	24.95329013261953
31	25.380276590380372	24.49090725642465	24.704936946753048	25.423879206441928
32	25.314378698958784	24.585344514210966	24.61136781926135	25.488908967568904
33	24.842053892139866	24.442617863563473	25.053340428693655	25.661987815603005
34	25.429821793221365	24.585909139373676	25.138602754475563	24.845666312929396
35	25.72818498190778	24.32409998067937	25.20855546094013	24.739159576472723
36	24.867391436055694	24.767353605822326	24.929670590738414	25.435584367383562
37	25.56574000063449	24.604320116135593	24.837817343579637	24.99212253965028
38	24.994645100135436	24.50006225518328	25.10050276427325	25.40478988040803
39	25.694453223560064	24.47310000789524	24.541724201346295	25.290722567198397
40	25.714205978506914	24.59676015130171	24.832924579562437	24.85610929062894
41	24.962604764502448	24.99313635527704	25.169074647115636	24.87518423310488
42	25.859930771878524	24.828617343478776	24.785227517498456	24.52622436714424
43	25.5682525658133	24.21491814157498	24.866527138964916	25.35030215364681
44	25.03521371174825	24.76328394786677	24.791645745169845	25.409856595215135
45	25.248758147328747	24.844161652543637	24.75299505644243	25.154085143685183
46	25.228154858112244	24.669467656156673	24.759322266769203	25.343055218961872
47	25.245785503443752	24.691684307820747	25.023310176212764	25.039220012522733
48	25.61649995050396	25.232124477160863	24.25389199845906	24.897483573876112
49	25.19587333770648	25.08920031009601	24.340175323513627	25.374751028683878
50	25.371999353106826	25.189404691311264	24.419549340631715	25.019046614950195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	125.0
1	101.0
2	77.0
3	228.0
4	379.0
5	426.5
6	474.0
7	480.5
8	487.0
9	643.0
10	799.0
11	1307.0
12	1815.0
13	3325.0
14	4835.0
15	6564.0
16	8293.0
17	8337.5
18	8382.0
19	7867.0
20	7352.0
21	8166.5
22	8981.0
23	10863.5
24	12746.0
25	16074.0
26	19402.0
27	24037.5
28	28673.0
29	34200.5
30	39728.0
31	47499.5
32	55271.0
33	65147.0
34	75023.0
35	89006.5
36	102990.0
37	121171.0
38	139352.0
39	157674.0
40	175996.0
41	193782.0
42	211568.0
43	223800.0
44	236032.0
45	258346.0
46	280660.0
47	298475.5
48	316291.0
49	330102.5
50	343914.0
51	337222.0
52	330530.0
53	328097.0
54	325664.0
55	324689.0
56	323714.0
57	311169.0
58	298624.0
59	272283.5
60	245943.0
61	216546.5
62	187150.0
63	160098.0
64	133046.0
65	112207.5
66	91369.0
67	77312.0
68	63255.0
69	54678.0
70	46101.0
71	35426.0
72	24751.0
73	21249.5
74	17748.0
75	13200.5
76	8653.0
77	7358.0
78	6063.0
79	5228.5
80	4394.0
81	3469.5
82	2545.0
83	2135.0
84	1725.0
85	1299.0
86	873.0
87	614.0
88	355.0
89	229.5
90	104.0
91	71.0
92	38.0
93	42.5
94	47.0
95	45.0
96	43.0
97	36.5
98	30.0
99	21.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032296367111898566
2	0.004102640430758163
3	1.9082048515154247E-4
4	9.541024257577123E-5
5	4.770512128788562E-5
6	1.4311536386365684E-4
7	0.0
8	0.0
9	0.0
10	9.541024257577123E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.825447438258839E-4
17	0.0
18	9.541024257577123E-5
19	0.0
20	2.862307277273137E-4
21	4.770512128788562E-5
22	0.0
23	9.541024257577123E-5
24	9.063973044698267E-4
25	9.541024257577123E-5
26	4.293460915909706E-4
27	2.623781670833709E-4
28	0.0015265638812123397
29	3.8164097030308493E-4
30	0.001264185714128969
31	3.100832883712565E-4
32	6.20166576742513E-4
33	2.623781670833709E-4
34	0.0010733652289774264
35	2.862307277273137E-4
36	1.9082048515154247E-4
37	0.0019320574121593674
38	0.0
39	5.486088948106847E-4
40	9.541024257577123E-5
41	0.0010256601076895407
42	0.004651249325568848
43	0.004388871158485477
44	0.003887967384962678
45	0.002313698382462452
46	0.002242140700530624
47	0.001121070350265312
48	0.003983377627538449
49	0.004102640430758163
50	0.0018127946089396534
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4192422.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.990040662935925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.38047916294187	34.2555209756943
2	15.809481994563182	15.173953675580806
3	5.478360068319843	7.88720167334621
4	2.4374494492338425	4.678931927303315
5	1.2387386655845347	2.97235594660764
6	0.7618677648442345	2.193723900879295
7	0.5199243693296157	1.7465834138045704
8	0.35076900199163624	1.346673493510086
9	0.2813092579404905	1.2150038454682024
>10	1.5715940507638595	13.695661536525611
>50	0.09799706327872171	3.2197817330589595
>100	0.06004694691602914	5.679112853847014
>500	0.007838358771614434	2.592650617481739
>1k	0.0041438455206410435	3.3428444068922634
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6696792450759967E-4
2	0.0	0.0	0.0	2.385256064394281E-5	1.6696792450759967E-4
3	0.0	0.0	0.0	2.385256064394281E-5	1.9082048515154247E-4
4	0.0	0.0	0.0	2.385256064394281E-5	1.9082048515154247E-4
5	0.0	0.0	0.0	2.385256064394281E-5	2.146730457954853E-4
6	7.155768193182842E-5	0.0	0.0	2.385256064394281E-5	2.385256064394281E-4
7	7.155768193182842E-5	0.0	0.0	2.385256064394281E-5	2.385256064394281E-4
8	7.155768193182842E-5	0.0	0.0	2.385256064394281E-5	2.385256064394281E-4
9	7.155768193182842E-5	0.0	0.0	2.385256064394281E-5	2.385256064394281E-4
10	7.155768193182842E-5	0.0	0.0	7.155768193182842E-5	2.385256064394281E-4
11	7.155768193182842E-5	0.0	0.0	7.155768193182842E-5	3.100832883712565E-4
12	7.155768193182842E-5	0.0	0.0	9.541024257577123E-5	6.917242586743415E-4
13	7.155768193182842E-5	0.0	0.0	9.541024257577123E-5	8.348396225379983E-4
14	7.155768193182842E-5	0.0	0.0	9.541024257577123E-5	8.348396225379983E-4
15	7.155768193182842E-5	0.0	0.0	1.1926280321971405E-4	0.0011449229109092547
16	9.541024257577123E-5	0.0	0.0	1.4311536386365684E-4	0.001240333153485026
17	9.541024257577123E-5	0.0	0.0	1.4311536386365684E-4	0.001264185714128969
18	9.541024257577123E-5	0.0	0.0	1.6696792450759967E-4	0.0013118908354168544
19	9.541024257577123E-5	0.0	0.0	1.6696792450759967E-4	0.0013118908354168544
20	9.541024257577123E-5	0.0	0.0	2.385256064394281E-4	0.0014073010779926257
21	9.541024257577123E-5	0.0	0.0	2.385256064394281E-4	0.0014550061992805114
22	9.541024257577123E-5	0.0	0.0	3.5778840965914216E-4	0.0015265638812123397
23	9.541024257577123E-5	0.0	0.0	3.8164097030308493E-4	0.0015265638812123397
24	9.541024257577123E-5	0.0	0.0	5.963140160985702E-4	0.0015265638812123397
25	9.541024257577123E-5	0.0	0.0	6.917242586743415E-4	0.0015265638812123397
26	9.541024257577123E-5	0.0	0.0	8.109870618940555E-4	0.0016935318057199394
27	9.541024257577123E-5	0.0	0.0	9.302498651137695E-4	0.0018127946089396534
28	9.541024257577123E-5	0.0	0.0	0.0015504164418562827	0.0018127946089396534
29	9.541024257577123E-5	0.0	0.0	0.0025522239889018807	0.0018127946089396534
30	9.541024257577123E-5	0.0	0.0	0.004579691643637019	0.0018127946089396534
31	9.541024257577123E-5	0.0	0.0	0.008992415362766439	0.00195590997280331
32	9.541024257577123E-5	0.0	0.0	0.0142638312650778	0.0019797625334472533
33	9.541024257577123E-5	0.0	0.0	0.01977377277382859	0.0019797625334472533
34	9.541024257577123E-5	0.0	0.0	0.025593797570950635	0.00212287789731091
35	9.541024257577123E-5	0.0	0.0	0.032487187597050105	0.0021467304579548528
36	9.541024257577123E-5	0.0	0.0	0.04152730808110443	0.0021467304579548528
37	9.541024257577123E-5	0.0	0.0	0.05726999810610668	0.0021467304579548528
38	9.541024257577123E-5	0.0	0.0	0.07513556602841985	0.0022182881398866813
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3055	0.0	27.515884	1
GTATCAA	6705	0.0	25.763344	1
TCAACGC	10030	0.0	17.06419	4
ATCAACG	10110	0.0	16.950922	3
TATCAAC	10260	0.0	16.853598	2
ACCGTCG	1005	0.0	16.417318	8
CAACGCA	10480	0.0	16.352465	5
AACGCAG	10530	0.0	16.29571	6
TAGGACG	405	0.0	16.29571	4
ATACCGT	1125	0.0	16.230526	6
GTATTAG	695	0.0	16.147924	1
TACCGTC	1055	0.0	15.639246	7
CGTCGTA	1100	0.0	15.199634	10
CCGTCGT	1110	0.0	14.864329	9
CGAGCCG	1290	0.0	14.325236	15
GTATACG	190	1.7507409E-7	13.898213	1
ACGCAGA	12570	0.0	13.651059	7
CGCAGAG	12955	0.0	13.313299	8
TTACCGC	1195	0.0	13.2549095	16
AGAGTAC	13225	0.0	13.108191	11
>>END_MODULE