FastQCFastQC Report
Thu 2 Feb 2017
SRR4062290_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062290_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132154
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18610.16437693105354925No Hit
TATCAACGCAGAGTACTTTTTTTTT11840.10457941234143057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA350.002169235416.28624516
TAACGAA400.005272141714.25235613
ACGAACG400.005275236414.25109615
GCGTTAT954.9294613E-913.9930311
CGCATCG1650.013.82046613
ATCGCCA1700.013.41220216
ATTCCGA500.001498239113.3010236
TCTATAC500.001501737913.2969063
TCTAGGA1153.783498E-1013.2137322
GTATAGG1153.8016879E-1013.2108111
GGTATCA5900.013.1968161
GCGTAAC801.9933323E-613.06523711
CGCCAGT1750.013.02726718
AACCGCG751.4759014E-512.66820057
AATTCCG604.089371E-412.667085
GCATCGC1900.012.501513514
CGTTATT1001.4462603E-712.34658052
CGCGTAA853.9333536E-612.2961510
CCGTCGT1650.012.0929079
AGGTCGA550.003064776812.0923737
Produced by FastQC (version 0.11.5)