##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062290_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1132154
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.215946770492355	32.0	32.0	32.0	32.0	32.0
2	30.747363874525902	32.0	32.0	32.0	32.0	32.0
3	30.780212762574703	32.0	32.0	32.0	32.0	32.0
4	30.78950566795683	32.0	32.0	32.0	32.0	32.0
5	30.705850087532262	32.0	32.0	32.0	32.0	32.0
6	34.34272899269887	36.0	36.0	36.0	32.0	36.0
7	34.28037881772268	36.0	36.0	36.0	32.0	36.0
8	34.237970276128515	36.0	36.0	36.0	32.0	36.0
9	34.37326459121286	36.0	36.0	36.0	32.0	36.0
10	34.05642783578912	36.0	36.0	36.0	32.0	36.0
11	34.35296169955677	36.0	36.0	36.0	32.0	36.0
12	34.169370068029615	36.0	36.0	36.0	32.0	36.0
13	34.25056926884505	36.0	36.0	36.0	32.0	36.0
14	34.16532821506615	36.0	36.0	36.0	32.0	36.0
15	34.120343168862185	36.0	36.0	36.0	32.0	36.0
16	34.1204085309949	36.0	36.0	36.0	32.0	36.0
17	34.049088728211885	36.0	36.0	36.0	32.0	36.0
18	34.06165150677381	36.0	36.0	36.0	32.0	36.0
19	34.063796091344464	36.0	36.0	36.0	32.0	36.0
20	34.04545759675804	36.0	36.0	36.0	32.0	36.0
21	34.03416408015164	36.0	36.0	36.0	32.0	36.0
22	34.00093273529926	36.0	36.0	36.0	32.0	36.0
23	33.96692764411909	36.0	36.0	36.0	32.0	36.0
24	33.94241684435156	36.0	36.0	36.0	32.0	36.0
25	33.58728406206223	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	18.0
5	110.0
6	247.0
7	49.0
8	192.0
9	136.0
10	90.0
11	25.0
12	42.0
13	47.0
14	159.0
15	249.0
16	398.0
17	532.0
18	764.0
19	1062.0
20	1561.0
21	2334.0
22	3552.0
23	5184.0
24	7289.0
25	10243.0
26	14105.0
27	17856.0
28	23277.0
29	30549.0
30	39993.0
31	54229.0
32	76106.0
33	106075.0
34	230443.0
35	505237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57030339806897	17.050761255424447	11.316061663588433	26.06287368291815
2	16.897819374076978	18.985348257124485	37.34224733074711	26.77458503805143
3	19.591625469130776	22.25223402967989	27.957411890858697	30.19872861033064
4	13.048044917775595	15.072825311124147	34.67550157947181	37.20362819162845
5	15.069578886260675	36.221136182699695	33.27548714939955	15.433797781640079
6	35.6315763888582	34.27619396949837	16.132983918367707	13.959245723275721
7	31.24148365082018	29.77921378567582	19.75722270413374	19.22207985937026
8	28.37912879853002	33.5078848844016	18.631742208404294	19.48124410866409
9	26.941872496219453	14.4904005164531	18.427824795054786	40.13990219227266
10	16.493827771974715	26.74865052992626	31.080929288033477	25.676592410065545
11	37.49416189593314	21.03592733860296	21.730573266436917	19.73933749902698
12	24.532684241902132	23.363311356918327	28.49356893559737	23.610435465582174
13	29.672738079023294	19.358265216414573	24.839694548464873	26.129302156097268
14	23.26765769669529	19.488596669643883	24.77038582962655	32.473359804034274
15	25.050193787822234	27.475946783086773	21.676463668542336	25.797395760548657
16	25.682090719038403	25.795020817341534	23.61539032957548	24.907498134044577
17	24.147689586557593	26.26203847004201	24.89082467388901	24.69944726951139
18	24.835367929575412	25.197129605475503	25.80295440175796	24.164548063191123
19	25.673029230542078	25.00948239810511	24.97942164886746	24.33806672248535
20	25.752610076766185	24.685858065223407	24.747391249380026	24.81414060863038
21	26.022086601190175	24.49745511642279	24.494449174382655	24.98600910800438
22	25.789731501015957	24.621337384190692	24.72151829340539	24.867412821387962
23	24.374792805774476	24.75439472765286	25.37109314568351	25.49971932088916
24	24.78226696973796	24.855205656152002	25.21892657240336	25.14360080170668
25	24.808176681675402	24.813039123204543	25.17560044522283	25.203183749897224
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	214.0
1	214.0
2	294.0
3	374.0
4	374.0
5	374.0
6	738.5
7	1103.0
8	1103.0
9	1103.0
10	1459.5
11	1816.0
12	1816.0
13	1816.0
14	2360.0
15	2904.0
16	2904.0
17	2904.0
18	4844.5
19	6785.0
20	6785.0
21	6785.0
22	10667.5
23	14550.0
24	14550.0
25	14550.0
26	21531.5
27	28513.0
28	28513.0
29	28513.0
30	36718.5
31	44924.0
32	44924.0
33	44924.0
34	55810.0
35	66696.0
36	66696.0
37	66696.0
38	78558.0
39	90420.0
40	90420.0
41	90420.0
42	103984.5
43	117549.0
44	117549.0
45	117549.0
46	130817.5
47	144086.0
48	144086.0
49	144086.0
50	149559.5
51	155033.0
52	155033.0
53	155033.0
54	147258.5
55	139484.0
56	139484.0
57	139484.0
58	128496.0
59	117508.0
60	117508.0
61	117508.0
62	102955.0
63	88402.0
64	88402.0
65	88402.0
66	71932.0
67	55462.0
68	55462.0
69	55462.0
70	42028.5
71	28595.0
72	28595.0
73	28595.0
74	21396.5
75	14198.0
76	14198.0
77	14198.0
78	11118.5
79	8039.0
80	8039.0
81	8039.0
82	5574.5
83	3110.0
84	3110.0
85	3110.0
86	2242.0
87	1374.0
88	1374.0
89	1374.0
90	922.0
91	470.0
92	470.0
93	470.0
94	291.5
95	113.0
96	113.0
97	113.0
98	272.5
99	432.0
100	432.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.715992700639665E-4
2	1.7665441273890303E-4
3	6.182904445861606E-4
4	0.011482536828028696
5	0.03683244505606128
6	0.06527380550702466
7	0.10961406310448932
8	0.11093897120003109
9	0.12047830948793184
10	0.13469898971341354
11	0.145739890509595
12	0.1367305154599109
13	0.13249080955417725
14	0.11871176536054281
15	0.1356705889834775
16	0.12065496390067075
17	0.1240113977427099
18	0.11473704107391751
19	0.09813152627646063
20	0.09336185713251025
21	0.09371516595798805
22	0.10599264764334179
23	0.08558906297199852
24	0.09424512919620476
25	0.09141865859238231
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1132154.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.67701377157499
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.96587475479078	48.52090483752482
2	12.267847049288507	14.88752648714509
3	3.4771452052517255	6.329483625144746
4	1.419115665266013	3.444308030592148
5	0.7499997486916731	2.275387254002122
6	0.4679263472285666	1.7035424048922312
7	0.29797494877041697	1.265616104908886
8	0.21010035524967036	1.0198609719117637
9	0.16556111684020172	0.9041178749895231
>10	0.858601824838403	9.323796031823742
>50	0.06616906474579368	2.7960306971742797
>100	0.05046581226820854	6.024908016563434
>500	0.002779274028655447	1.1337802272044994
>1k	4.388327413666495E-4	0.3707374361228428
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1861	0.16437693105354925	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1184	0.10457941234143057	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	8.83272063694515E-5
6	0.0	0.0	0.0	0.0	1.76654412738903E-4
7	0.0	0.0	0.0	0.0	1.76654412738903E-4
8	0.0	0.0	0.0	0.0	2.649816191083545E-4
9	0.0	0.0	0.0	0.0	2.649816191083545E-4
10	0.0	0.0	0.0	0.0	2.649816191083545E-4
11	0.0	0.0	0.0	0.0	2.649816191083545E-4
12	0.0	0.0	0.0	0.0	4.4163603184725755E-4
13	0.0	0.0	0.0	0.0	4.4163603184725755E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	35	0.0021692354	16.286245	16
TAACGAA	40	0.0052721417	14.252356	13
ACGAACG	40	0.0052752364	14.251096	15
GCGTTAT	95	4.9294613E-9	13.993031	1
CGCATCG	165	0.0	13.820466	13
ATCGCCA	170	0.0	13.412202	16
ATTCCGA	50	0.0014982391	13.301023	6
TCTATAC	50	0.0015017379	13.296906	3
TCTAGGA	115	3.783498E-10	13.213732	2
GTATAGG	115	3.8016879E-10	13.210811	1
GGTATCA	590	0.0	13.196816	1
GCGTAAC	80	1.9933323E-6	13.065237	11
CGCCAGT	175	0.0	13.027267	18
AACCGCG	75	1.4759014E-5	12.6682005	7
AATTCCG	60	4.089371E-4	12.66708	5
GCATCGC	190	0.0	12.5015135	14
CGTTATT	100	1.4462603E-7	12.3465805	2
CGCGTAA	85	3.9333536E-6	12.29615	10
CCGTCGT	165	0.0	12.092907	9
AGGTCGA	55	0.0030647768	12.092373	7
>>END_MODULE