FastQCFastQC Report
Thu 2 Feb 2017
SRR4062290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132154
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6250.024.998251
GTATCAA13900.024.0635681
GTCCTAC3600.020.782971
ATTCCGA1705.456968E-1219.41147443
GTACCGT1152.0214429E-719.1301466
GCATATA1509.564246E-917.60441
TAGGACG3750.017.5997354
TATAGGG1254.8525726E-717.5997334
TCAACGC18900.017.4600544
GTATAGG1408.346797E-817.2900351
TCCTACA5350.017.2722952
GTCCTAA2550.017.2592161
GTATAGT1154.262238E-617.2216951
AACGCAG19050.017.207096
CAACGCA19200.017.187245
GTAGGAC5400.017.1108553
ATCAACG19300.017.0981883
TAGTCGC1551.4390025E-817.03200133
ATACCGT2750.016.7997476
CGTCGTA2500.016.71974810