##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062290_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1132154
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255139318502607	32.0	32.0	32.0	32.0	32.0
2	31.42336378266561	32.0	32.0	32.0	32.0	32.0
3	31.554981036148792	32.0	32.0	32.0	32.0	32.0
4	31.636078660676905	32.0	32.0	32.0	32.0	32.0
5	31.6083827818477	32.0	32.0	32.0	32.0	32.0
6	35.243640882777434	36.0	36.0	36.0	36.0	36.0
7	35.24737712360686	36.0	36.0	36.0	36.0	36.0
8	35.200057589338556	36.0	36.0	36.0	36.0	36.0
9	35.28264794365431	36.0	36.0	36.0	36.0	36.0
10	35.160479934708526	36.0	36.0	36.0	36.0	36.0
11	35.28984661097342	36.0	36.0	36.0	36.0	36.0
12	35.20190892758406	36.0	36.0	36.0	36.0	36.0
13	35.242976662185534	36.0	36.0	36.0	36.0	36.0
14	35.206939161986796	36.0	36.0	36.0	36.0	36.0
15	35.1805593585325	36.0	36.0	36.0	36.0	36.0
16	35.19729559759538	36.0	36.0	36.0	36.0	36.0
17	35.17930511220205	36.0	36.0	36.0	36.0	36.0
18	35.171049168222694	36.0	36.0	36.0	36.0	36.0
19	35.166569212315636	36.0	36.0	36.0	36.0	36.0
20	35.163122684723106	36.0	36.0	36.0	36.0	36.0
21	35.15102185745049	36.0	36.0	36.0	36.0	36.0
22	35.131747094476545	36.0	36.0	36.0	36.0	36.0
23	35.10775919177073	36.0	36.0	36.0	36.0	36.0
24	35.0907782863462	36.0	36.0	36.0	36.0	36.0
25	35.0683908726198	36.0	36.0	36.0	36.0	36.0
26	35.03303967481456	36.0	36.0	36.0	36.0	36.0
27	35.011847328190335	36.0	36.0	36.0	36.0	36.0
28	34.994524596477156	36.0	36.0	36.0	36.0	36.0
29	34.9701922176665	36.0	36.0	36.0	36.0	36.0
30	34.9514103205041	36.0	36.0	36.0	36.0	36.0
31	34.95193145102168	36.0	36.0	36.0	36.0	36.0
32	34.92280378817723	36.0	36.0	36.0	32.0	36.0
33	34.912314932420855	36.0	36.0	36.0	32.0	36.0
34	34.89963644521858	36.0	36.0	36.0	32.0	36.0
35	34.88145340651537	36.0	36.0	36.0	32.0	36.0
36	34.86183681725278	36.0	36.0	36.0	32.0	36.0
37	34.84280053773603	36.0	36.0	36.0	32.0	36.0
38	34.821090593682484	36.0	36.0	36.0	32.0	36.0
39	34.8012134391611	36.0	36.0	36.0	32.0	36.0
40	34.790617707485026	36.0	36.0	36.0	32.0	36.0
41	34.771735117307365	36.0	36.0	36.0	32.0	36.0
42	34.74587114473826	36.0	36.0	36.0	32.0	36.0
43	34.745487804662616	36.0	36.0	36.0	32.0	36.0
44	34.696640209724116	36.0	36.0	36.0	32.0	36.0
45	34.680879986291615	36.0	36.0	36.0	32.0	36.0
46	34.65268152565817	36.0	36.0	36.0	32.0	36.0
47	34.63373445661986	36.0	36.0	36.0	32.0	36.0
48	34.60872637468048	36.0	36.0	36.0	32.0	36.0
49	34.59242028911261	36.0	36.0	36.0	32.0	36.0
50	34.176781603916076	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	32.0
22	77.0
23	223.0
24	537.0
25	1266.0
26	2744.0
27	5259.0
28	8939.0
29	14225.0
30	21760.0
31	32109.0
32	49051.0
33	83075.0
34	187716.0
35	725129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.21598668306544	17.555434214060533	11.692254407170221	26.536324695703804
2	16.292229117973886	19.368994557008314	37.0270948274796	27.3116814975382
3	19.031488760323278	22.586318067393897	28.2882126926644	30.093980479618427
4	12.846750530404874	15.453639699192866	34.810105339026315	36.88950443137594
5	14.746518135374048	36.50207745956373	33.14457775987677	15.606826645185452
6	35.77741465353531	34.414874354105024	16.056529611800556	13.751181380559114
7	31.27781202910558	29.7221932705268	19.727351579378777	19.272643120988842
8	28.396225248508593	33.56804816305909	18.572031720066352	19.463694868365963
9	27.061866141885293	14.256629398474061	18.330015174614054	40.351489285026595
10	16.457242490407648	26.605879775860487	31.18945159307231	25.747426140659556
11	37.647086880406725	20.93787594267211	21.774599568609922	19.640437608311238
12	24.623151974024733	23.25805499958486	28.526684532316278	23.592108494074125
13	29.853182517572698	19.13273282609963	24.922139567585326	26.091945088742346
14	23.312994521946663	19.253652771619407	24.792121919809496	32.64123078662443
15	25.08157017508219	27.42497928727011	21.74297842872966	25.750472108918043
16	25.890388695450937	25.757278654873133	23.574521307322556	24.77781134235337
17	24.30049268915713	26.141938287547458	24.882303997512707	24.67526502578271
18	24.99249217419569	25.002561493509706	25.80996191324133	24.194984419053274
19	25.745879094186837	24.916133317552205	25.020801057100005	24.317186531160957
20	25.86386444917683	24.522170629182856	24.848805503859467	24.765159417780843
21	26.228675604202255	24.299344435474328	24.582609786301155	24.88937017402226
22	25.964842238776704	24.410371733880726	24.874001240113977	24.750784787228593
23	24.5998335915432	24.462219804019593	25.362715672956153	25.57523093148105
24	24.938368875199952	24.650418367355954	25.37797920573357	25.03323355171052
25	24.981120009610024	24.460429748328625	25.356776613720257	25.201673628341098
26	24.661882856718886	25.302167906782834	25.47970590521414	24.55624333128414
27	25.2481561630526	24.898997482665724	24.968687894713597	24.884158459568077
28	24.9212337267157	24.721348022827424	25.2321474154858	25.125270834971076
29	24.755398803514378	25.02824274896679	25.157819332967655	25.058539114551177
30	24.77551402562576	24.948902169515836	25.41677634077704	24.858807464081362
31	25.29742057375739	24.71755092739396	24.79510242008354	25.18992607876511
32	25.150908098425468	24.842997281269376	24.69451819818292	25.311576422122233
33	24.63606950650663	24.784282689707133	25.36406298441995	25.21558481936629
34	25.271321253019714	24.668571605227246	25.485604759107712	24.57450238264533
35	25.47528953709396	24.739875917721296	25.318331814102702	24.466502731082045
36	24.61124953959403	24.96349874972729	25.23501682193131	25.19023488874737
37	25.461516990891553	24.727771221832008	24.93163365532056	24.87907813195588
38	24.827629456770016	24.827629456770016	25.141367693794308	25.20337339266566
39	25.485758903886957	24.62668242435163	24.869583958252733	25.017974713508686
40	25.785979645878566	24.564149400169942	25.084396645686013	24.565474308265483
41	24.800818982878383	24.90822657348587	25.498791664605662	24.79216277903008
42	25.56739988022196	24.925403720892433	25.18350949656124	24.323686902324365
43	25.098555346014724	24.579433886432795	25.23211186241559	25.08989890513689
44	24.71561548775985	24.920633798960157	25.088552717353856	25.275197995926135
45	24.8966547952175	25.219851465558662	24.935607987789364	24.947885751434466
46	24.875124875124875	24.893055662286432	25.031378877532724	25.200440585055972
47	25.00523347480645	24.92732757135854	25.226673497418595	24.840765456416417
48	25.44163618488522	25.21612547334725	24.539946665347582	24.802291676419955
49	25.102817409001293	25.18196208122222	24.621059549299357	25.09416096047713
50	24.980810422877333	25.54700231864856	24.625902616760516	24.84628464171359
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	107.0
1	72.5
2	38.0
3	113.0
4	188.0
5	179.0
6	170.0
7	152.0
8	134.0
9	171.5
10	209.0
11	376.0
12	543.0
13	939.0
14	1335.0
15	1710.5
16	2086.0
17	2067.5
18	2049.0
19	2003.0
20	1957.0
21	2358.5
22	2760.0
23	3376.0
24	3992.0
25	5173.5
26	6355.0
27	8033.0
28	9711.0
29	11619.0
30	13527.0
31	15630.5
32	17734.0
33	20949.5
34	24165.0
35	27945.0
36	31725.0
37	36249.5
38	40774.0
39	44137.0
40	47500.0
41	51306.0
42	55112.0
43	58145.0
44	61178.0
45	66204.0
46	71230.0
47	76275.5
48	81321.0
49	84822.5
50	88324.0
51	87695.5
52	87067.0
53	86284.5
54	85502.0
55	85509.0
56	85516.0
57	82133.5
58	78751.0
59	72380.5
60	66010.0
61	58384.5
62	50759.0
63	43767.0
64	36775.0
65	31024.5
66	25274.0
67	21374.0
68	17474.0
69	15480.5
70	13487.0
71	10523.0
72	7559.0
73	6687.0
74	5815.0
75	4248.0
76	2681.0
77	2404.0
78	2127.0
79	1788.0
80	1449.0
81	1108.0
82	767.0
83	622.0
84	477.0
85	368.0
86	259.0
87	175.5
88	92.0
89	63.0
90	34.0
91	29.0
92	24.0
93	17.0
94	10.0
95	9.0
96	8.0
97	8.0
98	8.0
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03197444870574144
2	0.004946323556689285
3	3.5330882547780605E-4
4	0.0
5	1.7665441273890303E-4
6	3.5330882547780605E-4
7	0.0
8	0.0
9	0.0
10	1.7665441273890303E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.066176509556121E-4
17	0.0
18	1.7665441273890303E-4
19	0.0
20	2.649816191083545E-4
21	0.0
22	0.0
23	0.0
24	0.0013249080955417727
25	2.649816191083545E-4
26	1.7665441273890303E-4
27	3.5330882547780605E-4
28	6.182904445861606E-4
29	4.416360318472575E-4
30	0.001059926476433418
31	2.649816191083545E-4
32	7.066176509556121E-4
33	8.832720636945151E-5
34	7.949448573250635E-4
35	1.7665441273890303E-4
36	8.832720636945151E-5
37	0.001589889714650127
38	0.0
39	7.066176509556121E-4
40	0.0
41	0.001589889714650127
42	0.005299632382167091
43	0.004328033112103124
44	0.005299632382167091
45	0.0022965073656057394
46	0.0020315257464973845
47	0.0016782169210195785
48	0.005034650763058736
49	0.004416360318472575
50	0.002561488984714094
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1132154.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.13519732402651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.80525860824979	49.58715491118565
2	12.562275320874944	15.611189118026259
3	3.4195757127719215	6.374280350325957
4	1.4435954385245624	3.58792349715153
5	0.7223747109054734	2.24424476019991
6	0.46579481432237346	1.7365351620257374
7	0.3044571385451135	1.3242253066146372
8	0.22356180398932132	1.1112845443993453
9	0.15050290203804995	0.8416374764375576
>10	0.7996606327292349	8.80521356019927
>50	0.057356863984770454	2.462685396998425
>100	0.04301380256199958	5.191549529867142
>500	0.0024293476968054077	1.0268903545672776
>1k	1.4290280569443574E-4	0.09518603200139399
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.649816191083545E-4
2	0.0	0.0	0.0	0.0	2.649816191083545E-4
3	0.0	0.0	0.0	0.0	2.649816191083545E-4
4	0.0	0.0	0.0	0.0	2.649816191083545E-4
5	0.0	0.0	0.0	0.0	2.649816191083545E-4
6	0.0	0.0	0.0	0.0	2.649816191083545E-4
7	0.0	0.0	0.0	0.0	2.649816191083545E-4
8	0.0	0.0	0.0	0.0	2.649816191083545E-4
9	0.0	0.0	0.0	0.0	2.649816191083545E-4
10	0.0	0.0	0.0	0.0	2.649816191083545E-4
11	0.0	0.0	0.0	0.0	2.649816191083545E-4
12	0.0	0.0	0.0	0.0	5.29963238216709E-4
13	0.0	0.0	0.0	0.0	5.29963238216709E-4
14	0.0	0.0	0.0	8.83272063694515E-5	5.29963238216709E-4
15	0.0	0.0	0.0	1.76654412738903E-4	7.949448573250636E-4
16	0.0	0.0	0.0	1.76654412738903E-4	7.949448573250636E-4
17	0.0	0.0	0.0	3.53308825477806E-4	7.949448573250636E-4
18	0.0	0.0	0.0	5.29963238216709E-4	7.949448573250636E-4
19	0.0	0.0	0.0	6.182904445861605E-4	8.832720636945151E-4
20	0.0	0.0	0.0	0.001059926476433418	8.832720636945151E-4
21	0.0	0.0	0.0	0.0011482536828028695	9.715992700639666E-4
22	0.0	0.0	0.0	0.0013249080955417725	0.0011482536828028695
23	0.0	0.0	0.0	0.0018548713337584817	0.0011482536828028695
24	0.0	0.0	0.0	0.0019431985401279332	0.0011482536828028695
25	0.0	0.0	0.0	0.002296507365605739	0.0011482536828028695
26	0.0	0.0	0.0	0.002826470603822448	0.001236580889172321
27	0.0	0.0	0.0	0.003091452222930803	0.0013249080955417725
28	0.0	0.0	0.0	0.004151378699364221	0.0013249080955417725
29	0.0	0.0	0.0	0.006359558858600509	0.0013249080955417725
30	0.0	8.83272063694515E-5	0.0	0.009627665494270214	0.0013249080955417725
31	0.0	8.83272063694515E-5	0.0	0.01589889714650127	0.0013249080955417725
32	0.0	8.83272063694515E-5	0.0	0.02526158102166313	0.0013249080955417725
33	0.0	8.83272063694515E-5	0.0	0.0322394303248498	0.0013249080955417725
34	0.0	8.83272063694515E-5	0.0	0.042485386263706175	0.001413235301911224
35	0.0	8.83272063694515E-5	0.0	0.05273134220256255	0.001413235301911224
36	0.0	8.83272063694515E-5	0.0	0.06580376874524137	0.001413235301911224
37	0.0	8.83272063694515E-5	0.0	0.08567739017836797	0.001413235301911224
38	0.0	8.83272063694515E-5	0.0	0.11005569913633657	0.0015898897146501272
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	625	0.0	24.99825	1
GTATCAA	1390	0.0	24.063568	1
GTCCTAC	360	0.0	20.78297	1
ATTCCGA	170	5.456968E-12	19.411474	43
GTACCGT	115	2.0214429E-7	19.130146	6
GCATATA	150	9.564246E-9	17.6044	1
TAGGACG	375	0.0	17.599735	4
TATAGGG	125	4.8525726E-7	17.599733	4
TCAACGC	1890	0.0	17.460054	4
GTATAGG	140	8.346797E-8	17.290035	1
TCCTACA	535	0.0	17.272295	2
GTCCTAA	255	0.0	17.259216	1
GTATAGT	115	4.262238E-6	17.221695	1
AACGCAG	1905	0.0	17.20709	6
CAACGCA	1920	0.0	17.18724	5
GTAGGAC	540	0.0	17.110855	3
ATCAACG	1930	0.0	17.098188	3
TAGTCGC	155	1.4390025E-8	17.032001	33
ATACCGT	275	0.0	16.799747	6
CGTCGTA	250	0.0	16.719748	10
>>END_MODULE