FastQCFastQC Report
Thu 2 Feb 2017
SRR4062289_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062289_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199319
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36680.1667788983771795No Hit
GCGCAAGACGGACCAGAGCGAAAGC32400.14731832899183794No Hit
GAATAGGACCGCGGTTCTATTTTGT24090.10953390572263505No Hit
GGGTAGGCACACGCTGAGCCAGTCA23910.1087154705615693No Hit
GATTAAGAGGGACGGCCGGGGGCAT23650.10753328644002985No Hit
TATCAACGCAGAGTACTTTTTTTTT23400.10639657093854961No Hit
TCGTAGTTCCGACCATAAACGATGC23280.1058509474978391No Hit
GAACTACGACGGTATCTGATCGTCT22920.10421407717570758No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG22250.10116767963174055No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCT508.715929E-515.2001273
GGTATCA10250.014.081191
CGCATCG3450.013.49409613
CGTTATT2400.013.060232
GTTATTC2750.012.7819253
AAGACGG6200.012.4122435
TAGGACA2400.012.2712164
GCATCGC3800.012.25066114
GCGTAAC2500.012.16176311
GCGTTAT2500.012.1542891
GTATCAA28700.012.1423791
CAAGACG6600.012.0912874
CTAGGAC2400.011.8750993
TCGCGTA2500.011.7817089
GCGCAAG6300.011.6056591
TCGCCAG4200.011.5347617
CGGACCA6950.011.483689
CGGTTCT4250.011.401134512
TACGCAG752.073081E-411.4008755
ATCGCCA4000.011.39983616
Produced by FastQC (version 0.11.5)