##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062289_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2199319
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.313110103627533	32.0	32.0	32.0	32.0	32.0
2	31.04372671722474	32.0	32.0	32.0	32.0	32.0
3	31.061175754858663	32.0	32.0	32.0	32.0	32.0
4	31.050108692736252	32.0	32.0	32.0	32.0	32.0
5	31.03289882004384	32.0	32.0	32.0	32.0	32.0
6	34.613689055566745	36.0	36.0	36.0	32.0	36.0
7	34.575411297769904	36.0	36.0	36.0	32.0	36.0
8	34.56363674391937	36.0	36.0	36.0	32.0	36.0
9	34.63633652053204	36.0	36.0	36.0	32.0	36.0
10	34.46794348614276	36.0	36.0	36.0	32.0	36.0
11	34.578997407833974	36.0	36.0	36.0	32.0	36.0
12	34.510524848828204	36.0	36.0	36.0	32.0	36.0
13	34.551032387752755	36.0	36.0	36.0	32.0	36.0
14	34.49615449145849	36.0	36.0	36.0	32.0	36.0
15	34.4709694228077	36.0	36.0	36.0	32.0	36.0
16	34.460416610778154	36.0	36.0	36.0	32.0	36.0
17	34.41455014029343	36.0	36.0	36.0	32.0	36.0
18	34.41163423768903	36.0	36.0	36.0	32.0	36.0
19	34.40261099003828	36.0	36.0	36.0	32.0	36.0
20	34.38615316832165	36.0	36.0	36.0	32.0	36.0
21	34.35994278228852	36.0	36.0	36.0	32.0	36.0
22	34.33706297267472	36.0	36.0	36.0	32.0	36.0
23	34.326587911985484	36.0	36.0	36.0	32.0	36.0
24	34.30190481690014	36.0	36.0	36.0	32.0	36.0
25	33.92755621171826	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	24.0
5	174.0
6	446.0
7	80.0
8	426.0
9	274.0
10	167.0
11	50.0
12	93.0
13	84.0
14	287.0
15	394.0
16	852.0
17	1130.0
18	1539.0
19	1940.0
20	2628.0
21	3491.0
22	4732.0
23	6680.0
24	9153.0
25	13242.0
26	18073.0
27	24739.0
28	33338.0
29	46031.0
30	62978.0
31	88984.0
32	132303.0
33	198721.0
34	475741.0
35	1070522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31532690792343	17.21379045682491	11.367537417774562	26.103345217477102
2	16.82007827902544	19.1512618491601	37.18381020973832	26.844849662076143
3	19.513463817156534	22.468628683100366	27.945957399211203	30.071950100531897
4	12.819972970662437	14.964312543540698	34.99721703321189	37.21849745258498
5	15.084768273368926	36.110866003633255	33.06385661303513	15.740509109962694
6	35.38447259311362	34.87194559615784	16.00453698998104	13.739044820747493
7	31.107059073070502	30.127939611483797	19.771558516395736	18.993442799049966
8	28.303592398992794	33.15986730827703	18.706929355494744	19.82961093723543
9	26.828033642701975	14.394746377801207	18.677107630789735	40.10011234870708
10	16.281487019109726	26.524468669713137	30.708183904633945	26.48586040654319
11	37.74046659317954	21.202630056053287	21.319781469132444	19.737121881634728
12	24.318153362850996	23.90352504638219	28.307909443755193	23.470412147011622
13	29.104312834247725	19.376090637216674	25.1710305813196	26.348565947216002
14	23.326515869153848	19.49727991828147	24.695592561756623	32.48061165080806
15	25.37497922789222	27.129943477102874	21.76229424231318	25.732783052691723
16	26.037718155817164	26.03011607512312	23.148699884876276	24.78346588418344
17	23.892535872969624	26.307679000655547	24.899391798382986	24.900393327991843
18	24.838419090285484	25.185683196268172	25.718437042961245	24.257460670485102
19	25.494135188323902	24.978244761903028	25.132670541951196	24.394949507821874
20	25.769136072721878	24.28663684525985	24.683077390783158	25.26114969123512
21	26.67036817912893	24.206799253629455	24.417694443180267	24.70513812406135
22	25.887588777083725	24.480172738676316	25.035501730481784	24.59673675375818
23	24.471107858957243	24.490447859812754	25.446571996224833	25.591872285005174
24	24.608890037754808	25.013379838131268	25.164926984311915	25.212803139802016
25	24.863143398958666	24.52833451580592	25.116946462890006	25.491575622345408
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	147.0
1	147.0
2	379.0
3	611.0
4	611.0
5	611.0
6	1363.0
7	2115.0
8	2115.0
9	2115.0
10	2564.5
11	3014.0
12	3014.0
13	3014.0
14	4116.5
15	5219.0
16	5219.0
17	5219.0
18	8748.5
19	12278.0
20	12278.0
21	12278.0
22	19766.5
23	27255.0
24	27255.0
25	27255.0
26	41405.5
27	55556.0
28	55556.0
29	55556.0
30	71196.0
31	86836.0
32	86836.0
33	86836.0
34	110423.5
35	134011.0
36	134011.0
37	134011.0
38	156301.0
39	178591.0
40	178591.0
41	178591.0
42	203838.5
43	229086.0
44	229086.0
45	229086.0
46	256194.0
47	283302.0
48	283302.0
49	283302.0
50	292594.0
51	301886.0
52	301886.0
53	301886.0
54	282404.5
55	262923.0
56	262923.0
57	262923.0
58	242962.0
59	223001.0
60	223001.0
61	223001.0
62	196250.0
63	169499.0
64	169499.0
65	169499.0
66	139278.0
67	109057.0
68	109057.0
69	109057.0
70	82335.0
71	55613.0
72	55613.0
73	55613.0
74	42799.0
75	29985.0
76	29985.0
77	29985.0
78	23732.0
79	17479.0
80	17479.0
81	17479.0
82	12133.5
83	6788.0
84	6788.0
85	6788.0
86	4944.0
87	3100.0
88	3100.0
89	3100.0
90	2024.5
91	949.0
92	949.0
93	949.0
94	573.0
95	197.0
96	197.0
97	197.0
98	509.0
99	821.0
100	821.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013640586017762773
2	3.182803404144646E-4
3	7.729665410065571E-4
4	0.010321376753440498
5	0.0335103729836372
6	0.06301950740206401
7	0.10662391403884566
8	0.10671485127896407
9	0.11762732009317431
10	0.13176806093158835
11	0.13713335809857505
12	0.13158618645135153
13	0.1264937010047201
14	0.11412623634861518
15	0.13022212784957526
16	0.11603591839110197
17	0.12181043313862154
18	0.11057968398399687
19	0.09762112726712223
20	0.09166473803936583
21	0.09180114389954344
22	0.10198611479280632
23	0.08188898472663583
24	0.09016427357741191
25	0.08848193463522118
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2199319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.29192066778639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.07032618446888	26.621003411193904
2	15.676761497326241	12.632936611561544
3	6.900993894746614	8.341628956080264
4	3.572153686088269	5.757157317320371
5	2.105205020732095	4.241137684238159
6	1.3595473439372154	3.286726423561079
7	0.8967373776661859	2.529188989653534
8	0.6377186908400253	2.055592871975272
9	0.4752635941845679	1.7234354729854473
>10	2.071634351618968	14.37081481356374
>50	0.1223387388787295	3.375122733959583
>100	0.09252457879493232	7.750260415061
>500	0.012907437601005024	3.529100288526976
>1k	0.005887603116247901	3.7858940103191165
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3668	0.1667788983771795	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3240	0.14731832899183794	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2409	0.10953390572263505	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2391	0.1087154705615693	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2365	0.10753328644002985	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2340	0.10639657093854961	No Hit
TCGTAGTTCCGACCATAAACGATGC	2328	0.1058509474978391	No Hit
GAACTACGACGGTATCTGATCGTCT	2292	0.10421407717570758	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2225	0.10116767963174055	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.546862005920924E-5	0.0
10	0.0	0.0	0.0	4.546862005920924E-5	0.0
11	0.0	0.0	0.0	4.546862005920924E-5	0.0
12	0.0	0.0	0.0	4.546862005920924E-5	0.0
13	0.0	0.0	0.0	4.546862005920924E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGCT	50	8.715929E-5	15.200127	3
GGTATCA	1025	0.0	14.08119	1
CGCATCG	345	0.0	13.494096	13
CGTTATT	240	0.0	13.06023	2
GTTATTC	275	0.0	12.781925	3
AAGACGG	620	0.0	12.412243	5
TAGGACA	240	0.0	12.271216	4
GCATCGC	380	0.0	12.250661	14
GCGTAAC	250	0.0	12.161763	11
GCGTTAT	250	0.0	12.154289	1
GTATCAA	2870	0.0	12.142379	1
CAAGACG	660	0.0	12.091287	4
CTAGGAC	240	0.0	11.875099	3
TCGCGTA	250	0.0	11.781708	9
GCGCAAG	630	0.0	11.605659	1
TCGCCAG	420	0.0	11.53476	17
CGGACCA	695	0.0	11.48368	9
CGGTTCT	425	0.0	11.4011345	12
TACGCAG	75	2.073081E-4	11.400875	5
ATCGCCA	400	0.0	11.399836	16
>>END_MODULE