FastQCFastQC Report
Thu 2 Feb 2017
SRR4062289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199319
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT30590.13908850876112105No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA25100.11412623634861518No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT25090.11408076772855599No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT23220.10557813577748384No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC22780.10357751649487865No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA22560.10257720685357603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32100.026.1886961
GGTATCA13700.022.4886321
TAGGACG2900.021.9992284
TTAGTAC1700.020.7051543
CGCAATA5500.019.99975236
TACGAAT5850.019.17968441
AACGCAG45500.018.614736
TCAACGC46000.018.4123954
TCTAGCG6100.018.3940528
TCGCATG600.007414515618.33310711
ATCAACG46200.018.332693
CAACGCA46900.018.0590675
ATACGAA6150.017.88595840
CAATACG6250.017.59978138
ATACCGT7000.017.599386
TACCGTC7300.017.4788367
TATCAAC49500.017.4224032
TATGCCG902.2166202E-417.11089929
CGTCGTA7200.017.11089910
AATACGA6450.017.05405239
Produced by FastQC (version 0.11.5)