##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062289_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2199319
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25929981053226	32.0	32.0	32.0	32.0	32.0
2	31.365493591425345	32.0	32.0	32.0	32.0	32.0
3	31.522288035523722	32.0	32.0	32.0	32.0	32.0
4	31.60870705886686	32.0	32.0	32.0	32.0	32.0
5	31.569345783854	32.0	32.0	32.0	32.0	32.0
6	35.201062692588025	36.0	36.0	36.0	36.0	36.0
7	35.209607610355754	36.0	36.0	36.0	36.0	36.0
8	35.145956998507266	36.0	36.0	36.0	36.0	36.0
9	35.25021654430303	36.0	36.0	36.0	36.0	36.0
10	35.10871774399257	36.0	36.0	36.0	36.0	36.0
11	35.24640763800067	36.0	36.0	36.0	36.0	36.0
12	35.1539694787341	36.0	36.0	36.0	36.0	36.0
13	35.20448875310949	36.0	36.0	36.0	36.0	36.0
14	35.15617698023797	36.0	36.0	36.0	36.0	36.0
15	35.128371100326966	36.0	36.0	36.0	36.0	36.0
16	35.146334842739954	36.0	36.0	36.0	36.0	36.0
17	35.1244808051947	36.0	36.0	36.0	36.0	36.0
18	35.12291532060606	36.0	36.0	36.0	36.0	36.0
19	35.11143858621691	36.0	36.0	36.0	36.0	36.0
20	35.11222382928534	36.0	36.0	36.0	36.0	36.0
21	35.09933847704676	36.0	36.0	36.0	36.0	36.0
22	35.085868398354215	36.0	36.0	36.0	36.0	36.0
23	35.05633380150856	36.0	36.0	36.0	36.0	36.0
24	35.032571900665616	36.0	36.0	36.0	36.0	36.0
25	35.020612744217644	36.0	36.0	36.0	36.0	36.0
26	34.9778831538308	36.0	36.0	36.0	36.0	36.0
27	34.968851721828436	36.0	36.0	36.0	36.0	36.0
28	34.944238648417986	36.0	36.0	36.0	32.0	36.0
29	34.91763814162475	36.0	36.0	36.0	32.0	36.0
30	34.90537707353958	36.0	36.0	36.0	32.0	36.0
31	34.90278581688241	36.0	36.0	36.0	32.0	36.0
32	34.877488440740066	36.0	36.0	36.0	32.0	36.0
33	34.85378064755499	36.0	36.0	36.0	32.0	36.0
34	34.83452832444952	36.0	36.0	36.0	32.0	36.0
35	34.82366587111738	36.0	36.0	36.0	32.0	36.0
36	34.80006038232744	36.0	36.0	36.0	32.0	36.0
37	34.79379662522808	36.0	36.0	36.0	32.0	36.0
38	34.77723149756811	36.0	36.0	36.0	32.0	36.0
39	34.75316859446038	36.0	36.0	36.0	32.0	36.0
40	34.75086469948197	36.0	36.0	36.0	32.0	36.0
41	34.71869792422109	36.0	36.0	36.0	32.0	36.0
42	34.68436229578337	36.0	36.0	36.0	32.0	36.0
43	34.68509934211453	36.0	36.0	36.0	32.0	36.0
44	34.6193362581781	36.0	36.0	36.0	32.0	36.0
45	34.613374412715935	36.0	36.0	36.0	32.0	36.0
46	34.59028453807747	36.0	36.0	36.0	32.0	36.0
47	34.56686456125737	36.0	36.0	36.0	32.0	36.0
48	34.54216964433081	36.0	36.0	36.0	32.0	36.0
49	34.51997413744891	36.0	36.0	36.0	32.0	36.0
50	34.08226273678352	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	6.0
21	41.0
22	125.0
23	348.0
24	937.0
25	2310.0
26	5108.0
27	10134.0
28	17564.0
29	28648.0
30	44263.0
31	66222.0
32	101894.0
33	174896.0
34	395305.0
35	1351514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.96031687192449	17.787363601755104	11.831301222541262	26.42101830377915
2	16.31373108932663	19.657415057422607	36.854731743649225	27.174122109601534
3	18.68995573621787	22.9379602285257	28.33232165469703	30.039762380559402
4	12.52642295183191	15.44387148931101	35.16038373696585	36.86932182189123
5	14.849532695407433	36.412064665589064	32.812762155489324	15.925640483514186
6	35.433048668812184	35.03256015284766	15.949518804499949	13.584872373840206
7	31.17978792526232	30.05894097218275	19.822226789292504	18.939044313262425
8	28.379739364776096	33.15080713620898	18.650364044506503	19.81908945450842
9	26.91187590340465	14.100319235181436	18.5392387370818	40.448566124332125
10	16.278273537523905	26.486710880372915	30.79149991042678	26.443515671676405
11	38.090154270480994	21.00241029154934	21.325692180170318	19.581743257799346
12	24.357585234338448	23.669008452161783	28.371054858344785	23.60235145515498
13	29.27947241850773	19.313660273930246	25.085446904246268	26.321420403315756
14	23.503138926185787	19.28365098469117	24.693962085536477	32.51924800358657
15	25.428462173972942	27.035959767546224	21.755688920070256	25.77988913841057
16	26.11388800350657	25.93183015876408	23.08383436373648	24.870447473992876
17	24.107007669192146	26.20947666072998	24.7881275976791	24.895388072398774
18	24.973343997250055	25.124572765090253	25.658465787333068	24.243617450326624
19	25.70536607013353	24.817182045896935	25.06775961104324	24.4096922729263
20	25.961824390376982	24.090692080698037	24.626360085663233	25.32112344326175
21	26.887095505472374	24.14015429321531	24.362950531505433	24.609799669806883
22	26.13004298148654	24.284289818803	25.001875580577444	24.583791619133013
23	24.566377137650335	24.338579351153697	25.47320329611121	25.621840215084763
24	24.839824253411194	24.83532282727755	25.175476048548106	25.149376870763145
25	25.03270336445666	24.288198825543407	25.157651359627884	25.52144645037205
26	24.67299986222958	25.38772370074082	25.416187160433427	24.52308927659617
27	25.300230799869777	24.789888455823377	24.98108495945088	24.928795784855964
28	24.8628644595241	24.443318825581418	25.635661424354474	25.058155290540014
29	24.69800714497794	24.802449135113925	25.551275924643534	24.9482677952646
30	24.601634067613425	24.96147632629881	25.65915381142247	24.777735794665297
31	25.236062781537292	24.748182269976617	24.71649049769723	25.29926445078886
32	25.10665927641292	24.628232800268083	24.73808628500159	25.527021638317404
33	24.57450329921357	24.595282524716822	25.352019176906232	25.47819499916337
34	25.191196503627506	24.567864073608607	25.512024691402935	24.728914731360955
35	25.78565478658781	24.245036629636903	25.403035130986417	24.566273452788874
36	24.53334291208209	25.11834377371479	24.939833484365344	25.40847982983777
37	25.53583494219467	24.774630583309616	25.013936391852237	24.675598082643486
38	24.70119159612589	24.50226638336685	25.41241175109204	25.384130269415216
39	25.621426129088583	24.473649749738097	24.716136436386915	25.188787684786405
40	25.62683239122053	24.63315800577635	25.14281739016565	24.597192212837466
41	24.660152148895122	25.31113407808782	25.415940486329013	24.61277328668804
42	25.846397150969167	25.06693330859711	24.955802190247017	24.130867350186705
43	25.240344719555008	24.361039760607884	25.22806773440061	25.170547785436494
44	24.6422486487273	25.03570579245488	24.94030846348915	25.381737095328667
45	24.887747098475636	25.248047652067157	24.86705846244785	24.997146787009356
46	24.923917763434602	24.96420394995601	24.816654655077947	25.295223631531442
47	24.91075549210088	24.850281567502314	25.309610579753965	24.92935236064284
48	25.647476616815883	25.3840662413663	24.272722725681263	24.695734416136556
49	24.854063354555798	25.32645338712156	24.570238291193057	25.249244967129584
50	25.08643722229598	25.323605318821947	24.47869397494821	25.11126348393386
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	65.0
1	62.5
2	60.0
3	145.0
4	230.0
5	254.0
6	278.0
7	240.5
8	203.0
9	297.5
10	392.0
11	572.0
12	752.0
13	1472.5
14	2193.0
15	2781.0
16	3369.0
17	3653.5
18	3938.0
19	4019.5
20	4101.0
21	4861.0
22	5621.0
23	6571.0
24	7521.0
25	9518.5
26	11516.0
27	14711.5
28	17907.0
29	21357.5
30	24808.0
31	28795.0
32	32782.0
33	38731.5
34	44681.0
35	52082.0
36	59483.0
37	70315.5
38	81148.0
39	90170.0
40	99192.0
41	108290.5
42	117389.0
43	120975.0
44	124561.0
45	132729.0
46	140897.0
47	149474.0
48	158051.0
49	164933.0
50	171815.0
51	166169.0
52	160523.0
53	158813.5
54	157104.0
55	158064.5
56	159025.0
57	154972.0
58	150919.0
59	139716.5
60	128514.0
61	113161.0
62	97808.0
63	84684.0
64	71560.0
65	60911.5
66	50263.0
67	43571.5
68	36880.0
69	32636.0
70	28392.0
71	22449.0
72	16506.0
73	14193.0
74	11880.0
75	8960.0
76	6040.0
77	5098.5
78	4157.0
79	3505.5
80	2854.0
81	2259.0
82	1664.0
83	1383.5
84	1103.0
85	909.5
86	716.0
87	493.5
88	271.0
89	173.5
90	76.0
91	59.0
92	42.0
93	31.5
94	21.0
95	22.0
96	23.0
97	18.0
98	13.0
99	12.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03191897128156489
2	0.004455924765802506
3	1.8187448023683695E-4
4	0.0
5	1.8187448023683695E-4
6	2.2734310029604618E-4
7	0.0
8	0.0
9	0.0
10	4.546862005920924E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.184351610657663E-4
17	0.0
18	9.093724011841848E-5
19	0.0
20	4.092175805328831E-4
21	0.0
22	0.0
23	0.0
24	7.274979209473478E-4
25	1.8187448023683695E-4
26	3.637489604736739E-4
27	5.001548206513015E-4
28	0.0015914017020723233
29	5.456234407105109E-4
30	0.001000309641302603
31	4.5468620059209235E-4
32	0.0011821841215394402
33	3.182803404144646E-4
34	5.001548206513015E-4
35	3.182803404144646E-4
36	2.7281172035525545E-4
37	0.0018642134224275786
38	0.0
39	0.0010457782613618124
40	3.182803404144646E-4
41	0.0011821841215394402
42	0.005228891306809062
43	0.003728426844855157
44	0.004364987525684087
45	0.002000619282605206
46	0.0024553054831972987
47	0.0010912468814210218
48	0.004046707185269622
49	0.003501083744559111
50	0.0015914017020723233
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2199319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.001219785463846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.16434218035103	27.789830778775592
2	15.929151268491907	13.380875668476575
3	6.856466564666628	8.639398774027418
4	3.4944506252360616	5.870847549599654
5	2.0853608031563713	4.379384871268107
6	1.3447169795291762	3.388785204387
7	0.8804542472555534	2.5886106645017937
8	0.62700498116971	2.106797921655169
9	0.4531033156839311	1.712780275280688
>10	1.965761773455697	14.043700321976615
>50	0.10303624829680004	2.99591482107375
>100	0.08080123008337493	6.9554242430024695
>500	0.010777506948573639	3.120211536870331
>1k	0.004572275675152446	3.0274373691049417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3059	0.13908850876112105	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2510	0.11412623634861518	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2509	0.11408076772855599	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2322	0.10557813577748384	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2278	0.10357751649487865	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2256	0.10257720685357603	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	4.546862005920924E-5
18	0.0	0.0	0.0	0.0	4.546862005920924E-5
19	0.0	0.0	0.0	4.546862005920924E-5	4.546862005920924E-5
20	0.0	0.0	0.0	4.546862005920924E-5	4.546862005920924E-5
21	0.0	0.0	0.0	4.546862005920924E-5	4.546862005920924E-5
22	0.0	0.0	0.0	2.2734310029604618E-4	4.546862005920924E-5
23	0.0	0.0	0.0	4.5468620059209235E-4	4.546862005920924E-5
24	0.0	0.0	0.0	6.365606808289294E-4	4.546862005920924E-5
25	0.0	0.0	0.0	7.729665410065571E-4	4.546862005920924E-5
26	0.0	4.546862005920924E-5	0.0	9.093724011841847E-4	4.546862005920924E-5
27	0.0	4.546862005920924E-5	0.0	0.0010457782613618124	4.546862005920924E-5
28	0.0	4.546862005920924E-5	0.0	0.0013185899817170679	4.546862005920924E-5
29	0.0	4.546862005920924E-5	0.0	0.0026371799634341358	4.546862005920924E-5
30	0.0	4.546862005920924E-5	0.0	0.004274050285565668	4.546862005920924E-5
31	0.0	4.546862005920924E-5	0.0	0.009184661251960265	4.546862005920924E-5
32	0.0	4.546862005920924E-5	0.0	0.01491370737942063	4.546862005920924E-5
33	0.0	4.546862005920924E-5	0.0	0.01977884972575602	4.546862005920924E-5
34	0.0	4.546862005920924E-5	0.0	0.02614445653404531	4.546862005920924E-5
35	0.0	4.546862005920924E-5	0.0	0.033192092643222745	4.546862005920924E-5
36	0.0	4.546862005920924E-5	0.0	0.04205847355476854	4.546862005920924E-5
37	0.0	4.546862005920924E-5	0.0	0.05651749473359708	4.546862005920924E-5
38	0.0	4.546862005920924E-5	0.0	0.07475041137733998	4.546862005920924E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3210	0.0	26.188696	1
GGTATCA	1370	0.0	22.488632	1
TAGGACG	290	0.0	21.999228	4
TTAGTAC	170	0.0	20.705154	3
CGCAATA	550	0.0	19.999752	36
TACGAAT	585	0.0	19.179684	41
AACGCAG	4550	0.0	18.61473	6
TCAACGC	4600	0.0	18.412395	4
TCTAGCG	610	0.0	18.39405	28
TCGCATG	60	0.0074145156	18.333107	11
ATCAACG	4620	0.0	18.33269	3
CAACGCA	4690	0.0	18.059067	5
ATACGAA	615	0.0	17.885958	40
CAATACG	625	0.0	17.599781	38
ATACCGT	700	0.0	17.59938	6
TACCGTC	730	0.0	17.478836	7
TATCAAC	4950	0.0	17.422403	2
TATGCCG	90	2.2166202E-4	17.110899	29
CGTCGTA	720	0.0	17.110899	10
AATACGA	645	0.0	17.054052	39
>>END_MODULE