FastQCFastQC Report
Thu 2 Feb 2017
SRR4062288_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062288_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293543
Sequences flagged as poor quality0
Sequence length25
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGGGTAGGGGAATTCCGA22560.7685415765322287No Hit
CAAGTAGAGTGATCGAAAGATGAAA21830.7436729882845103No Hit
CTCTACTTGTGCGCTATCGGTCTCT15080.5137237133912237No Hit
CTACTAGATAGTTCGATTAGTCTTT12820.43673328950102713No Hit
GTTCTAAGTTGGTCGTTAAGCGTGC12520.42651332172799217No Hit
ATCTAGTAGCTGGTTCCTGCCGAAG12140.4135680292154812No Hit
CCCTTGGGGGTTCCCACCTCCGTTC12060.4108427044760052No Hit
CATCAGGATCGGTCGATGGTGCACC11380.3876774441904593No Hit
ATCCTGATGTCTTCGGATGGATTTG10960.3733694893082104No Hit
GGTTAAGGTTGTTTCAACCCCAAGG10510.3580395376486579No Hit
CTCCTTAGCGGATTCCGACTTCCAT8440.28752176001471674No Hit
ATTCAGGCATAGTTCACCATCTTTC8370.28513710086767524No Hit
GTATCAACGCAGAGTACTTTTTTTT8250.2810491137584613No Hit
GCTAAATACCGGCCAGAGACCGATA8080.2752577986870748No Hit
CTGCTATCCTGAGGGAAACTTCGGC7930.27014781480055733No Hit
ACCTTAACCTATTCTCAAACTTTAA7870.26810382124595034No Hit
ATGTAGGTAAGGGAAGTCGGCAAAA7830.26674115887621236No Hit
CTCCACGGCAACGTAACCGAAGGCG7660.2609498438048259No Hit
CCTTAACCCCGCGTTCGGTTCATCC7540.25686185669561185No Hit
GTGCTGGAGGGCGCCGGCCGGTTGC7510.2558398599183084No Hit
GGCTATAACGCACCCCGAGGGGTGC7500.25549919432587387No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA7420.2527738695863979No Hit
GTCTAACATCTACGCGAGTGTTTGG7390.2517518728090944No Hit
CCCCCAAGGGTGCACCATCGACCGA7280.24800455129231494No Hit
GTTATAGCCCGGGGCGCAATGCGGC7150.24357589859066645No Hit
CGTCAGAACCGCTGCGAGCCTCCAC7130.24289456740579746No Hit
GTCTAGATGAACTAACGCCTTTTGT7030.23948791148145246No Hit
GGTAGGGATACCCGCTGAACTTAAG7020.23914724588901798No Hit
GTTCTGACGTGCAAATCGATCGTCA6980.23778458351927997No Hit
ATGTTAGACTCCTTGGTCCGTGTTT6630.22586128778407252No Hit
CCCCCAGGGGGTCCGAGTTGTAATT6590.22449862541433452No Hit
GGATAGCAGTAACGACCTTCAGTTT6540.222795297452162No Hit
GCCTCTAGTTGATAGAACAATGTAG6410.21836664475051357No Hit
GTATTTAGCTTTAGAAGGAATTTAC6340.21598198560347204No Hit
TATCAACGCAGAGTACTTTTTTTTT6060.2064433490153061No Hit
CCTTTGCGGCGTCCGGTGCGCTCCC5650.19247605972549167No Hit
CCCCGAGGGGTGCTACGTTCCCGGG5620.19145406294818818No Hit
CTCTTACTCAAATCCATCCGAAGAC5580.1900914005784502No Hit
GCTCTAAGCGGGAGGTAAATTCCTT5570.1897507349860157No Hit
CCTTAGCGGATTCCGACTTCCATGG5510.18770674143140867No Hit
CCGCAAAGGTGCGGCGCTCTGCCGG5500.1873660758389742No Hit
GGGTAGGGGAATTCCGATTCAACGT5490.18702541024653968No Hit
GTACTTTTTTTTTTTTTTTTTTTTT5450.1856627478768017No Hit
TTATAGCCCGGGGCGCAATGCGGCC5390.1836187543221947No Hit
GAGTAAGAGCGTAGCTGTTGGGACC5320.1812340951751532No Hit
TCCCACCTCCGTTCACTTTCATTGC5210.17748677365837373No Hit
CCTTAGGACCGACTAACCCGTGTCC5210.17748677365837373No Hit
GTGCTACGTTCCCGGGGCCTTTGTC5180.17646477688107023No Hit
GGTTTGACACCCAAACACTCGCGTA5120.17442078332646324No Hit
TGCCTGAATAGGGTGAAGCCAGAGG5050.17203612417942177No Hit
GATCTGCACCAGGGGCCGTTCGACC5020.17101412740211824No Hit
CCTTGGAACAGGGCGTCGTAGAGGG4980.16965146503238027No Hit
GGGTTAAGGTGCCGGAATGCACGCT4980.16965146503238027No Hit
GTATCAACGCAGAGTACATGGGCAG4920.16760747147777327No Hit
GTTTGGGAATGCAGCTCTAAGCGGG4810.1638601499609938No Hit
GTTACACACTCCTTAGCGGATTCCG4730.1611348252215178No Hit
GGGCTATAACGCACCCCGAGGGGTG4730.1611348252215178No Hit
GAATAGGGTGAAGCCAGAGGAAACT4710.16045349403664882No Hit
GTACATGGGGTTGACCTCGGATCAG4660.1587501660744763No Hit
ACTAGAGGCTGTTCACCTTGGAGAC4650.1584095004820418No Hit
ACGTAGCACCCCTCGGGGTGCGTTA4610.15704683811230383No Hit
CCCCTTGTCCGTACCAGTTCTAAGT4580.15602484133500033No Hit
CTATAACGCACCCCGAGGGGTGCTA4570.15568417574256582No Hit
GGTTAGTCGGTCCTAAGGGGTAGGG4540.15466217896526233No Hit
GTGCCTACCTGCTCCGGGAGCTTCT4540.15466217896526233No Hit
GCGTTATAGCCCGGGGCGCAATGCG4420.15057419185604834No Hit
CTTGAGCGCCATCCATTTTCAGGGC4400.14989286067117935No Hit
GTCCGGGCTGGCCGCATTGCGCCCC4400.14989286067117935No Hit
GAACTTAAGCATATCAATAAGCGGA4330.14750820152413788No Hit
GTCCGTACCAGTTCTAAGTTGGTCG4320.14716753593170337No Hit
TTGTTACACACTCCTTAGCGGATTC4280.14580487356196536No Hit
GTTTTAATTAGACAGTCAGATTCCC4220.14376088000735837No Hit
GGCCAGCATCGGTTCGGGCGGCCGG4210.1434202144149239No Hit
GTCGTAGAGGGTGAGAACCCCGTAT4170.1420575520451859No Hit
AGCCCGGACCGAGGACCGCGCTTCG4140.1410355552678824No Hit
TTCCTGGACGGGCGGCGGGGTATTG4110.1400135584905789No Hit
GGTGAGAACCCCGTATGCGGCCGGC4100.13967289289814439No Hit
TACCTACATTGTTCTATCAACTAGA4070.1386508961208409No Hit
TCGCAATGCTATGTTTTAATTAGAC4060.1383102305284064No Hit
GGTAGGCACTAGCCGGGCAACCGGC4010.13660690256623392No Hit
GAGGTGGGAACCCCCAAGGGTGCAC3960.1349035746040614No Hit
TATTTAAAGTTTGAGAATAGGTTAA3950.1345629090116269No Hit
GGCTTCACCCTATTCAGGCATAGTT3930.1338815778267579No Hit
CCCCCGAGGCTTCGTCACCGACCCC3920.1335409122343234No Hit
CTAGTAACGGTACATTCGATTGTCC3910.13320024664188893No Hit
TTCCCAAACAACTCGACTCGTCGAA3900.13285958104945442No Hit
CATCAGACACCACAAAAGGCGTTAG3900.13285958104945442No Hit
TTTCAAGACGGGTCGCTTACGACCA3860.13149691867971644No Hit
TGATAGAACAATGTAGGTAAGGGAA3860.13149691867971644No Hit
ACCCGGGGTCACCCCCGAGGCTTCG3850.13115625308728193No Hit
CTTTAAATATGTAAGAAGTCCTTGT3790.12911225953267494No Hit
GCTTAGAGCTGCATTCCCAAACAAC3790.12911225953267494No Hit
GTCCTGCTGTCTAGATGAACTAACG3780.12877159394024043No Hit
ATGGTGAACTATGCCTGAATAGGGT3760.12809026275537144No Hit
CCGTTACTAGTGGGCCATTTTTGGT3740.12740893157050245No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3730.12706826597806795No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3720.12672760038563344No Hit
GATTTGAGTAAGAGCGTAGCTGTTG3720.12672760038563344No Hit
CATCTAGACAGCAGGACGGTGGCCA3710.12638693479319896No Hit
GTATTGGTAGCACGCTTGAGCGCCA3690.12570560360832997No Hit
GCCGAAGCCTGCCGAGGGCGTCCGC3690.12570560360832997No Hit
GTTCACCATCTTTCGGGTCCCAACA3640.12400227564615747No Hit
GTACATGGGCAGAAATCACATTGCG3640.12400227564615747No Hit
CCTTTTGTGGTGTCTGATGAGCGTG3630.12366161005372296No Hit
GCCAGAGACCGATAGCGCACAAGTA3590.12229894768398498No Hit
TCTCCGGACAAACCGATTTCAGGGT3580.12195828209155048No Hit
ATGCCTGAATAGGGTGAAGCCAGAG3560.12127695090668147No Hit
GTCCGGAGATAGGGTTTAACGGCCG3520.11991428853694347No Hit
GTCGTTAAGCGTGCGCCGGACGGCC3510.11957362294450899No Hit
GTCTTGAAACACGGACCAAGGAGTC3510.11957362294450899No Hit
CCCTATCTCCGGACAAACCGATTTC3500.11923295735207448No Hit
GTCAGATTCCCCTTGTCCGTACCAG3480.11855162616720549No Hit
ACTCAAATCCATCCGAAGACATCAG3460.11787029498233649No Hit
GAGATAGGGTTTAACGGCCGGCAGA3430.116848298205033No Hit
GGGTATTGGTAGCACGCTTGAGCGC3430.116848298205033No Hit
CAGTAACGGCGAGTGAAGCGGCAAC3400.1158263014277295No Hit
GAACAATGTAGGTAAGGGAAGTCGG3370.11480430465042599No Hit
GCCGTGGAGAATCCACATCCAGGTG3360.1144636390579915No Hit
CCCCAAGGGTGCACCATCGACCGAT3350.114122973465557No Hit
GATTAGAGGCCTTGGGGTTGAAACA3340.11378230787312252No Hit
TGTCTGATGAGCGTGCATTCCGGCA3330.11344164228068801No Hit
GTTCTCACCCTCTACGACGCCCTGT3310.11276031109581902No Hit
GGTATCAACGCAGAGTACTTTTTTT3310.11276031109581902No Hit
GATGTGGATTCTCCACGGCAACGTA3290.11207897991095002No Hit
TATCAACGCAGAGTACATGGGCAGA3220.10969432076390852No Hit
ACTCATAACCGCAGCAGGTCTCCAA3210.10935365517147402No Hit
GGCGTAATGGTCGTAAGCGACCCGT3180.10833165839417053No Hit
TGTAAGAAGTCCTTGTTTCTTAGTT3090.10526566806226005No Hit
GTCTCTGGCCGGTATTTAGCTTTAG3080.10492500246982554No Hit
TTCCGGCACCTTAACCCCGCGTTCG3070.10458433687739106No Hit
CTAGTTGATAGAACAATGTAGGTAA3070.10458433687739106No Hit
CCTCTAATCATTCGCTTTACCTCAT3050.10390300569252205No Hit
CTTTCATTGCGCGCACGGGTTTGAC3040.10356234010008755No Hit
GACCGATAGCGCACAAGTAGAGTGA3040.10356234010008755No Hit
TCTCCAAGGTGAACAGCCTCTAGTT3030.10322167450765304No Hit
GCTAAGGAGTGTGTAACAACTCACC3030.10322167450765304No Hit
CCCCGTATGCGGCCGGCGGCCGAGC3020.10288100891521856No Hit
GTTCCAAGGAACTTAGACCGGGGCC3020.10288100891521856No Hit
ATGGTGCACCCTTGGGGGTTCCCAC3000.10219967773034955No Hit
GTGCACCCTTGGGGGTTCCCACCTC2990.10185901213791507No Hit
AGGTAGGGATACCCGCTGAACTTAA2990.10185901213791507No Hit
GGGTTTAACGGCCGGCAGAGCGCCG2990.10185901213791507No Hit
CCACAAAAGGCGTTAGTTCATCTAG2980.10151834654548056No Hit
GTCTAAGTTCCTTGGAACAGGGCGT2970.10117768095304608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA1550.017.7756313
AGAAGGA701.2951205E-917.64428113
AGGAATT701.2951205E-917.64428116
AAGGAAT701.2951205E-917.64428115
GAAGGAA701.2951205E-917.64428114
CTTTAGA753.2414391E-916.4679999
ACCGGCG350.002163388516.287038
CGTCCAG350.002163388516.2870318
GTTAAGC1750.015.74412815
GATCGGT2000.015.6762677
GGATCGG2000.015.6762676
CGGTCGA2000.015.67626710
TCGGTCG2000.015.6762679
AACGTAA1100.015.54671110
TTAGAAG807.603376E-915.441380511
TAGAAGG807.603376E-915.441380512
TTTAGAA807.621566E-915.43874710
AGGATCG2100.015.3795735
ATCAGGA2050.015.2887042
CAGGATC2050.015.2887044
Produced by FastQC (version 0.11.5)