##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062288_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	293543
Sequences flagged as poor quality	0
Sequence length	25
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178457670596814	32.0	32.0	32.0	32.0	32.0
2	30.747754162081876	32.0	32.0	32.0	32.0	32.0
3	30.81427593231656	32.0	32.0	32.0	32.0	32.0
4	30.76110825330531	32.0	32.0	32.0	32.0	32.0
5	30.73276487601476	32.0	32.0	32.0	32.0	32.0
6	34.340413499896094	36.0	36.0	36.0	32.0	36.0
7	34.2280483608875	36.0	36.0	36.0	32.0	36.0
8	34.18616352629768	36.0	36.0	36.0	32.0	36.0
9	34.30712706485932	36.0	36.0	36.0	32.0	36.0
10	34.03127650804141	36.0	36.0	36.0	32.0	36.0
11	34.29921680980299	36.0	36.0	36.0	32.0	36.0
12	34.122390927393944	36.0	36.0	36.0	32.0	36.0
13	34.245149773627716	36.0	36.0	36.0	32.0	36.0
14	34.17552453984595	36.0	36.0	36.0	32.0	36.0
15	34.08052993939559	36.0	36.0	36.0	32.0	36.0
16	34.029201854583484	36.0	36.0	36.0	32.0	36.0
17	33.9300749804969	36.0	36.0	36.0	32.0	36.0
18	34.01292144592104	36.0	36.0	36.0	32.0	36.0
19	33.95452114340999	36.0	36.0	36.0	32.0	36.0
20	33.938768766415826	36.0	36.0	36.0	32.0	36.0
21	33.92339452822925	36.0	36.0	36.0	32.0	36.0
22	33.88200365874846	36.0	36.0	36.0	32.0	36.0
23	33.867382291521174	36.0	36.0	36.0	32.0	36.0
24	33.81141093468418	36.0	36.0	36.0	32.0	36.0
25	33.60852072779797	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	6.0
5	35.0
6	52.0
7	9.0
8	46.0
9	40.0
10	31.0
11	6.0
12	5.0
13	11.0
14	36.0
15	62.0
16	110.0
17	192.0
18	298.0
19	456.0
20	649.0
21	838.0
22	1169.0
23	1535.0
24	2078.0
25	2676.0
26	3609.0
27	4546.0
28	6030.0
29	7788.0
30	10193.0
31	13597.0
32	19452.0
33	27617.0
34	59649.0
35	130722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.023012117557684	17.049066399583023	9.852116058744775	28.07580542411452
2	19.53853438848823	18.122046855145584	35.12330391118167	27.21611484518452
3	19.725761395380527	19.86202902500511	29.548272807794508	30.863936771819855
4	14.539104955622562	13.718676002112401	33.305395138072605	38.43682390419244
5	16.16197759057823	34.431994765682504	33.18168806739184	16.224339576347425
6	35.389418930849125	35.47736158405852	14.495539807685098	14.63767967740726
7	32.143015464356964	31.829295323182894	17.061601677720756	18.96608753473939
8	30.60824313356386	31.028038658018403	17.37360096576842	20.990117242649315
9	30.406831800338995	12.5879468107238	17.969285546199572	39.03593584273763
10	20.501519704996436	22.61341502103026	28.10379704518862	28.78126822878468
11	39.903739557856895	20.518278227470724	18.895290333364034	20.68269188130835
12	24.6799758510954	21.957766703845067	26.714555172402033	26.647702272657504
13	29.012926771035847	18.786793546846756	25.786350148367955	26.413929533749446
14	25.817904023954792	17.635623885218898	22.577663793956056	33.96880829687026
15	30.64260863633263	23.102871955795074	19.04461423016577	27.209905177706528
16	26.767394270122786	27.1343792633015	19.819918144611187	26.278308321964527
17	24.638773672433555	26.037971402062983	22.641966381051137	26.681288544452325
18	26.44422922151441	23.879741914362494	24.11027295420753	25.56575590991556
19	28.5948108012262	23.86799562167747	23.159075642000527	24.3781179350958
20	29.016012220570385	21.42862013939088	23.256250085243934	26.299117554794798
21	25.17373306145274	21.422872068361144	27.050868494813585	26.35252637537253
22	27.689228083098033	22.312476981626222	22.23574906903466	27.762545866241084
23	25.912023020483865	22.61817090799989	27.13464528271009	24.335160788806153
24	28.155346426623023	23.34526732133115	23.049986361156574	25.44939989088925
25	26.31313355177693	24.009587878085288	22.38762713655907	27.289651433578715
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	822.0
1	822.0
2	505.5
3	189.0
4	189.0
5	189.0
6	450.0
7	711.0
8	711.0
9	711.0
10	665.0
11	619.0
12	619.0
13	619.0
14	447.5
15	276.0
16	276.0
17	276.0
18	886.5
19	1497.0
20	1497.0
21	1497.0
22	1858.5
23	2220.0
24	2220.0
25	2220.0
26	3928.5
27	5637.0
28	5637.0
29	5637.0
30	7262.5
31	8888.0
32	8888.0
33	8888.0
34	10634.0
35	12380.0
36	12380.0
37	12380.0
38	15214.0
39	18048.0
40	18048.0
41	18048.0
42	21456.5
43	24865.0
44	24865.0
45	24865.0
46	30845.5
47	36826.0
48	36826.0
49	36826.0
50	39256.0
51	41686.0
52	41686.0
53	41686.0
54	39492.0
55	37298.0
56	37298.0
57	37298.0
58	33243.5
59	29189.0
60	29189.0
61	29189.0
62	23277.5
63	17366.0
64	17366.0
65	17366.0
66	18672.5
67	19979.0
68	19979.0
69	19979.0
70	15989.5
71	12000.0
72	12000.0
73	12000.0
74	11833.0
75	11666.0
76	11666.0
77	11666.0
78	9512.0
79	7358.0
80	7358.0
81	7358.0
82	5046.0
83	2734.0
84	2734.0
85	2734.0
86	1816.5
87	899.0
88	899.0
89	899.0
90	546.0
91	193.0
92	193.0
93	193.0
94	127.0
95	61.0
96	61.0
97	61.0
98	98.5
99	136.0
100	136.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.81331184868997E-4
2	0.0
3	0.0010219967773034957
4	0.012945292512510944
5	0.03236323128127736
6	0.05791315071386475
7	0.09811169062113557
8	0.10390300569252205
9	0.11105698313364652
10	0.13422224341919242
11	0.12945292512510945
12	0.12332094446128845
13	0.12025495412937798
14	0.11037565194877753
15	0.12366161005372296
16	0.116848298205033
17	0.12774959716293693
18	0.10458433687739106
19	0.0953863658816596
20	0.0909577131800111
21	0.09436436910435608
22	0.10185901213791507
23	0.08141907659184515
24	0.08959505081027311
25	0.08721039166323162
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	293543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.995608820513517
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.48659298983733	17.818513812286447
2	11.774465941749874	5.415220257338788
3	3.5747088975141477	2.466078223633335
4	1.539213652928802	1.415806202157776
5	0.7718289828449527	0.8874338682918685
6	0.47109715267695773	0.6499899503650232
7	0.3614707712364078	0.5818568318781234
8	0.27110307842730585	0.49873442732410583
9	0.213327012532962	0.44150260779511014
>10	2.1303072501555507	10.931277530038189
>50	0.6014636603359901	9.997513141175228
>100	0.7288672928209535	34.90561859761602
>500	0.06073894106841278	9.15777245582419
>1k	0.014814375870344582	4.832682094275796
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAGGGGTAGGGGAATTCCGA	2256	0.7685415765322287	No Hit
CAAGTAGAGTGATCGAAAGATGAAA	2183	0.7436729882845103	No Hit
CTCTACTTGTGCGCTATCGGTCTCT	1508	0.5137237133912237	No Hit
CTACTAGATAGTTCGATTAGTCTTT	1282	0.43673328950102713	No Hit
GTTCTAAGTTGGTCGTTAAGCGTGC	1252	0.42651332172799217	No Hit
ATCTAGTAGCTGGTTCCTGCCGAAG	1214	0.4135680292154812	No Hit
CCCTTGGGGGTTCCCACCTCCGTTC	1206	0.4108427044760052	No Hit
CATCAGGATCGGTCGATGGTGCACC	1138	0.3876774441904593	No Hit
ATCCTGATGTCTTCGGATGGATTTG	1096	0.3733694893082104	No Hit
GGTTAAGGTTGTTTCAACCCCAAGG	1051	0.3580395376486579	No Hit
CTCCTTAGCGGATTCCGACTTCCAT	844	0.28752176001471674	No Hit
ATTCAGGCATAGTTCACCATCTTTC	837	0.28513710086767524	No Hit
GTATCAACGCAGAGTACTTTTTTTT	825	0.2810491137584613	No Hit
GCTAAATACCGGCCAGAGACCGATA	808	0.2752577986870748	No Hit
CTGCTATCCTGAGGGAAACTTCGGC	793	0.27014781480055733	No Hit
ACCTTAACCTATTCTCAAACTTTAA	787	0.26810382124595034	No Hit
ATGTAGGTAAGGGAAGTCGGCAAAA	783	0.26674115887621236	No Hit
CTCCACGGCAACGTAACCGAAGGCG	766	0.2609498438048259	No Hit
CCTTAACCCCGCGTTCGGTTCATCC	754	0.25686185669561185	No Hit
GTGCTGGAGGGCGCCGGCCGGTTGC	751	0.2558398599183084	No Hit
GGCTATAACGCACCCCGAGGGGTGC	750	0.25549919432587387	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	742	0.2527738695863979	No Hit
GTCTAACATCTACGCGAGTGTTTGG	739	0.2517518728090944	No Hit
CCCCCAAGGGTGCACCATCGACCGA	728	0.24800455129231494	No Hit
GTTATAGCCCGGGGCGCAATGCGGC	715	0.24357589859066645	No Hit
CGTCAGAACCGCTGCGAGCCTCCAC	713	0.24289456740579746	No Hit
GTCTAGATGAACTAACGCCTTTTGT	703	0.23948791148145246	No Hit
GGTAGGGATACCCGCTGAACTTAAG	702	0.23914724588901798	No Hit
GTTCTGACGTGCAAATCGATCGTCA	698	0.23778458351927997	No Hit
ATGTTAGACTCCTTGGTCCGTGTTT	663	0.22586128778407252	No Hit
CCCCCAGGGGGTCCGAGTTGTAATT	659	0.22449862541433452	No Hit
GGATAGCAGTAACGACCTTCAGTTT	654	0.222795297452162	No Hit
GCCTCTAGTTGATAGAACAATGTAG	641	0.21836664475051357	No Hit
GTATTTAGCTTTAGAAGGAATTTAC	634	0.21598198560347204	No Hit
TATCAACGCAGAGTACTTTTTTTTT	606	0.2064433490153061	No Hit
CCTTTGCGGCGTCCGGTGCGCTCCC	565	0.19247605972549167	No Hit
CCCCGAGGGGTGCTACGTTCCCGGG	562	0.19145406294818818	No Hit
CTCTTACTCAAATCCATCCGAAGAC	558	0.1900914005784502	No Hit
GCTCTAAGCGGGAGGTAAATTCCTT	557	0.1897507349860157	No Hit
CCTTAGCGGATTCCGACTTCCATGG	551	0.18770674143140867	No Hit
CCGCAAAGGTGCGGCGCTCTGCCGG	550	0.1873660758389742	No Hit
GGGTAGGGGAATTCCGATTCAACGT	549	0.18702541024653968	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	545	0.1856627478768017	No Hit
TTATAGCCCGGGGCGCAATGCGGCC	539	0.1836187543221947	No Hit
GAGTAAGAGCGTAGCTGTTGGGACC	532	0.1812340951751532	No Hit
TCCCACCTCCGTTCACTTTCATTGC	521	0.17748677365837373	No Hit
CCTTAGGACCGACTAACCCGTGTCC	521	0.17748677365837373	No Hit
GTGCTACGTTCCCGGGGCCTTTGTC	518	0.17646477688107023	No Hit
GGTTTGACACCCAAACACTCGCGTA	512	0.17442078332646324	No Hit
TGCCTGAATAGGGTGAAGCCAGAGG	505	0.17203612417942177	No Hit
GATCTGCACCAGGGGCCGTTCGACC	502	0.17101412740211824	No Hit
CCTTGGAACAGGGCGTCGTAGAGGG	498	0.16965146503238027	No Hit
GGGTTAAGGTGCCGGAATGCACGCT	498	0.16965146503238027	No Hit
GTATCAACGCAGAGTACATGGGCAG	492	0.16760747147777327	No Hit
GTTTGGGAATGCAGCTCTAAGCGGG	481	0.1638601499609938	No Hit
GTTACACACTCCTTAGCGGATTCCG	473	0.1611348252215178	No Hit
GGGCTATAACGCACCCCGAGGGGTG	473	0.1611348252215178	No Hit
GAATAGGGTGAAGCCAGAGGAAACT	471	0.16045349403664882	No Hit
GTACATGGGGTTGACCTCGGATCAG	466	0.1587501660744763	No Hit
ACTAGAGGCTGTTCACCTTGGAGAC	465	0.1584095004820418	No Hit
ACGTAGCACCCCTCGGGGTGCGTTA	461	0.15704683811230383	No Hit
CCCCTTGTCCGTACCAGTTCTAAGT	458	0.15602484133500033	No Hit
CTATAACGCACCCCGAGGGGTGCTA	457	0.15568417574256582	No Hit
GGTTAGTCGGTCCTAAGGGGTAGGG	454	0.15466217896526233	No Hit
GTGCCTACCTGCTCCGGGAGCTTCT	454	0.15466217896526233	No Hit
GCGTTATAGCCCGGGGCGCAATGCG	442	0.15057419185604834	No Hit
CTTGAGCGCCATCCATTTTCAGGGC	440	0.14989286067117935	No Hit
GTCCGGGCTGGCCGCATTGCGCCCC	440	0.14989286067117935	No Hit
GAACTTAAGCATATCAATAAGCGGA	433	0.14750820152413788	No Hit
GTCCGTACCAGTTCTAAGTTGGTCG	432	0.14716753593170337	No Hit
TTGTTACACACTCCTTAGCGGATTC	428	0.14580487356196536	No Hit
GTTTTAATTAGACAGTCAGATTCCC	422	0.14376088000735837	No Hit
GGCCAGCATCGGTTCGGGCGGCCGG	421	0.1434202144149239	No Hit
GTCGTAGAGGGTGAGAACCCCGTAT	417	0.1420575520451859	No Hit
AGCCCGGACCGAGGACCGCGCTTCG	414	0.1410355552678824	No Hit
TTCCTGGACGGGCGGCGGGGTATTG	411	0.1400135584905789	No Hit
GGTGAGAACCCCGTATGCGGCCGGC	410	0.13967289289814439	No Hit
TACCTACATTGTTCTATCAACTAGA	407	0.1386508961208409	No Hit
TCGCAATGCTATGTTTTAATTAGAC	406	0.1383102305284064	No Hit
GGTAGGCACTAGCCGGGCAACCGGC	401	0.13660690256623392	No Hit
GAGGTGGGAACCCCCAAGGGTGCAC	396	0.1349035746040614	No Hit
TATTTAAAGTTTGAGAATAGGTTAA	395	0.1345629090116269	No Hit
GGCTTCACCCTATTCAGGCATAGTT	393	0.1338815778267579	No Hit
CCCCCGAGGCTTCGTCACCGACCCC	392	0.1335409122343234	No Hit
CTAGTAACGGTACATTCGATTGTCC	391	0.13320024664188893	No Hit
TTCCCAAACAACTCGACTCGTCGAA	390	0.13285958104945442	No Hit
CATCAGACACCACAAAAGGCGTTAG	390	0.13285958104945442	No Hit
TTTCAAGACGGGTCGCTTACGACCA	386	0.13149691867971644	No Hit
TGATAGAACAATGTAGGTAAGGGAA	386	0.13149691867971644	No Hit
ACCCGGGGTCACCCCCGAGGCTTCG	385	0.13115625308728193	No Hit
CTTTAAATATGTAAGAAGTCCTTGT	379	0.12911225953267494	No Hit
GCTTAGAGCTGCATTCCCAAACAAC	379	0.12911225953267494	No Hit
GTCCTGCTGTCTAGATGAACTAACG	378	0.12877159394024043	No Hit
ATGGTGAACTATGCCTGAATAGGGT	376	0.12809026275537144	No Hit
CCGTTACTAGTGGGCCATTTTTGGT	374	0.12740893157050245	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	373	0.12706826597806795	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	372	0.12672760038563344	No Hit
GATTTGAGTAAGAGCGTAGCTGTTG	372	0.12672760038563344	No Hit
CATCTAGACAGCAGGACGGTGGCCA	371	0.12638693479319896	No Hit
GTATTGGTAGCACGCTTGAGCGCCA	369	0.12570560360832997	No Hit
GCCGAAGCCTGCCGAGGGCGTCCGC	369	0.12570560360832997	No Hit
GTTCACCATCTTTCGGGTCCCAACA	364	0.12400227564615747	No Hit
GTACATGGGCAGAAATCACATTGCG	364	0.12400227564615747	No Hit
CCTTTTGTGGTGTCTGATGAGCGTG	363	0.12366161005372296	No Hit
GCCAGAGACCGATAGCGCACAAGTA	359	0.12229894768398498	No Hit
TCTCCGGACAAACCGATTTCAGGGT	358	0.12195828209155048	No Hit
ATGCCTGAATAGGGTGAAGCCAGAG	356	0.12127695090668147	No Hit
GTCCGGAGATAGGGTTTAACGGCCG	352	0.11991428853694347	No Hit
GTCGTTAAGCGTGCGCCGGACGGCC	351	0.11957362294450899	No Hit
GTCTTGAAACACGGACCAAGGAGTC	351	0.11957362294450899	No Hit
CCCTATCTCCGGACAAACCGATTTC	350	0.11923295735207448	No Hit
GTCAGATTCCCCTTGTCCGTACCAG	348	0.11855162616720549	No Hit
ACTCAAATCCATCCGAAGACATCAG	346	0.11787029498233649	No Hit
GAGATAGGGTTTAACGGCCGGCAGA	343	0.116848298205033	No Hit
GGGTATTGGTAGCACGCTTGAGCGC	343	0.116848298205033	No Hit
CAGTAACGGCGAGTGAAGCGGCAAC	340	0.1158263014277295	No Hit
GAACAATGTAGGTAAGGGAAGTCGG	337	0.11480430465042599	No Hit
GCCGTGGAGAATCCACATCCAGGTG	336	0.1144636390579915	No Hit
CCCCAAGGGTGCACCATCGACCGAT	335	0.114122973465557	No Hit
GATTAGAGGCCTTGGGGTTGAAACA	334	0.11378230787312252	No Hit
TGTCTGATGAGCGTGCATTCCGGCA	333	0.11344164228068801	No Hit
GTTCTCACCCTCTACGACGCCCTGT	331	0.11276031109581902	No Hit
GGTATCAACGCAGAGTACTTTTTTT	331	0.11276031109581902	No Hit
GATGTGGATTCTCCACGGCAACGTA	329	0.11207897991095002	No Hit
TATCAACGCAGAGTACATGGGCAGA	322	0.10969432076390852	No Hit
ACTCATAACCGCAGCAGGTCTCCAA	321	0.10935365517147402	No Hit
GGCGTAATGGTCGTAAGCGACCCGT	318	0.10833165839417053	No Hit
TGTAAGAAGTCCTTGTTTCTTAGTT	309	0.10526566806226005	No Hit
GTCTCTGGCCGGTATTTAGCTTTAG	308	0.10492500246982554	No Hit
TTCCGGCACCTTAACCCCGCGTTCG	307	0.10458433687739106	No Hit
CTAGTTGATAGAACAATGTAGGTAA	307	0.10458433687739106	No Hit
CCTCTAATCATTCGCTTTACCTCAT	305	0.10390300569252205	No Hit
CTTTCATTGCGCGCACGGGTTTGAC	304	0.10356234010008755	No Hit
GACCGATAGCGCACAAGTAGAGTGA	304	0.10356234010008755	No Hit
TCTCCAAGGTGAACAGCCTCTAGTT	303	0.10322167450765304	No Hit
GCTAAGGAGTGTGTAACAACTCACC	303	0.10322167450765304	No Hit
CCCCGTATGCGGCCGGCGGCCGAGC	302	0.10288100891521856	No Hit
GTTCCAAGGAACTTAGACCGGGGCC	302	0.10288100891521856	No Hit
ATGGTGCACCCTTGGGGGTTCCCAC	300	0.10219967773034955	No Hit
GTGCACCCTTGGGGGTTCCCACCTC	299	0.10185901213791507	No Hit
AGGTAGGGATACCCGCTGAACTTAA	299	0.10185901213791507	No Hit
GGGTTTAACGGCCGGCAGAGCGCCG	299	0.10185901213791507	No Hit
CCACAAAAGGCGTTAGTTCATCTAG	298	0.10151834654548056	No Hit
GTCTAAGTTCCTTGGAACAGGGCGT	297	0.10117768095304608	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAA	155	0.0	17.77563	13
AGAAGGA	70	1.2951205E-9	17.644281	13
AGGAATT	70	1.2951205E-9	17.644281	16
AAGGAAT	70	1.2951205E-9	17.644281	15
GAAGGAA	70	1.2951205E-9	17.644281	14
CTTTAGA	75	3.2414391E-9	16.467999	9
ACCGGCG	35	0.0021633885	16.28703	8
CGTCCAG	35	0.0021633885	16.28703	18
GTTAAGC	175	0.0	15.744128	15
GATCGGT	200	0.0	15.676267	7
GGATCGG	200	0.0	15.676267	6
CGGTCGA	200	0.0	15.676267	10
TCGGTCG	200	0.0	15.676267	9
AACGTAA	110	0.0	15.546711	10
TTAGAAG	80	7.603376E-9	15.4413805	11
TAGAAGG	80	7.603376E-9	15.4413805	12
TTTAGAA	80	7.621566E-9	15.438747	10
AGGATCG	210	0.0	15.379573	5
ATCAGGA	205	0.0	15.288704	2
CAGGATC	205	0.0	15.288704	4
>>END_MODULE