FastQCFastQC Report
Thu 2 Feb 2017
SRR4062288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293543
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTCAAAAA24010.8179380874352309No Hit
GTCCTAAGGGGTAGGGGAATTCCGATTCAACGTGCGCGCTCGCCGCGCCG19250.6557812654364097No Hit
GTTCTAAGTTGGTCGTTAAGCGTGCGCCGGACGGCCGAAGCCTGCCGAGG14300.4871517971813329No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGGAA14110.4806791509250774No Hit
CTACTAGATAGTTCGATTAGTCTTTCGCCCCTATACCCAAATTTGACGAT13040.4442279325345861No Hit
CCCTTGGGGGTTCCCACCTCCGTTCACTTTCATTGCGCGCACGGGTTTGA12810.4363926239085926No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGAC11690.39823807755592877No Hit
CATCAGGATCGGTCGATGGTGCACCCTTGGGGGTTCCCACCTCCGTTCAC11210.3818861291190728No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCAT10150.34577557632101596No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCGTAGCTGTTGGGACCC9930.33828093328745706No Hit
GTGCTGGAGGGCGCCGGCCGGTTGCCCGGCTAGTGCCTACCTGCTCCGGG9210.31375301063217315No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGGCCTG8440.28752176001471674No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTACGCTCTTACT8430.28718109442228223No Hit
CCCCCAGGGGGTCCGAGTTGTAATTTGCAGAGGATGCTTCGGGCGAGGCC8010.2728731395400333No Hit
CGTCAGAACCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTA7850.2674224900610814No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGA7780.2650378309140399No Hit
GCTAAATACCGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGA7630.25992784702752236No Hit
CCCCCAAGGGTGCACCATCGACCGATCCTGATGTCTTCGGATGGATTTGA7570.25788385347291537No Hit
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATAGTTCGA7510.2558398599183084No Hit
CCTTAACCCCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAA7480.2548178631410049No Hit
TACCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG7350.2503892104393564No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA7330.24970787925448742No Hit
GTCTAACATCTACGCGAGTGTTTGGGTGTCAAACCCGTGCGCGCAATGAA7320.24936721366205292No Hit
ATGTAGGTAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA7270.2476638856998804No Hit
GGCTATAACGCACCCCGAGGGGTGCTACGTTCCCGGGGCCTTTGTCCGGC7270.2476638856998804No Hit
GTCTAGATGAACTAACGCCTTTTGTGGTGTCTGATGAGCGTGCATTCCGG6890.23471859318736948No Hit
ATGTTAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGCTTACGACCATTA6660.22688328456137602No Hit
GTTATAGCCCGGGGCGCAATGCGGCCAGCCCGGACCGAGGACCGCGCTTC6500.22143263508242403No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTTCTTA6500.22143263508242403No Hit
GGATAGCAGTAACGACCTTCAGTTTTATGAGGTAAAGCGAATGATTAGAG6480.22075130389755507No Hit
GTTCTGACGTGCAAATCGATCGTCAAATTTGGGTATAGGGGCGAAAGACT6470.22041063830512053No Hit
CCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAAC6030.2054213522380026No Hit
TTATAGCCCGGGGCGCAATGCGGCCAGCCCGGACCGAGGACCGCGCTTCG5810.19792670920444366No Hit
GCTCTAAGCGGGAGGTAAATTCCTTCTAAAGCTAAATACCGGCCAGAGAC5700.19417938768766416No Hit
GTATTTAGCTTTAGAAGGAATTTACCTCCCGCTTAGAGCTGCATTCCCAA5620.19145406294818818No Hit
CTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCGATGGTGCACCC5430.18498141669193272No Hit
GGTTTGACACCCAAACACTCGCGTAGATGTTAGACTCCTTGGTCCGTGTT5380.1832780887297602No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGT5360.1825967575448912No Hit
CCCCGAGGGGTGCTACGTTCCCGGGGCCTTTGTCCGGCCGCCCGAACCGA5310.1808934295827187No Hit
GGGTAGGGGAATTCCGATTCAACGTGCGCGCTCGCCGCGCCGCCCCCCGA5260.17919010162054622No Hit
TCCCACCTCCGTTCACTTTCATTGCGCGCACGGGTTTGACACCCAAACAC5220.17782743925080824No Hit
GATCTGCACCAGGGGCCGTTCGACCCGGGGTCACCCCCGAGGCTTCGTCA5210.17748677365837373No Hit
GAGTAAGAGCGTAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAA5100.17373945214159425No Hit
GTGCCTACCTGCTCCGGGAGCTTCTGCCCAAGGCCCAACGCACCCGACCC5080.17305812095672526No Hit
CCGCAAAGGTGCGGCGCTCTGCCGGCCGTTAAACCCTATCTCCGGACAAA5010.17067346180968376No Hit
GCCTCTAGTTGATAGAACAATGTAGGTAAGGGAAGTCGGCAAAATAGATC4950.16862946825507677No Hit
GTGCTACGTTCCCGGGGCCTTTGTCCGGCCGCCCGAACCGATGCTGGCCC4900.16692614029290428No Hit
GTTTGGGAATGCAGCTCTAAGCGGGAGGTAAATTCCTTCTAAAGCTAAAT4880.1662448091080353No Hit
TGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGCAGCGG4860.16556347792316628No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG4800.1635194843685593No Hit
ACGTAGCACCCCTCGGGGTGCGTTATAGCCCGGGGCGCAATGCGGCCAGC4790.16317881877612478No Hit
GGGTTAAGGTGCCGGAATGCACGCTCATCAGACACCACAAAAGGCGTTAG4730.1611348252215178No Hit
CCCCCGAGGCTTCGTCACCGACCCCCGCGCCTGCCTACTCGCCTGTCCAT4720.1607941596290833No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTCGTTAAGCGTGCGCCGGACGG4700.16011282844421432No Hit
GAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGCAGCGGTTCTG4700.16011282844421432No Hit
CTCCACGGCAACGTAACCGAAGGCGGAGACGTCGGCGGGGGCCCTGGGAA4640.1580688348896073No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCC4590.1563655069274348No Hit
GTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGC4560.15534351015013134No Hit
CCTTGGAACAGGGCGTCGTAGAGGGTGAGAACCCCGTATGCGGCCGGCGG4560.15534351015013134No Hit
GTCCGTACCAGTTCTAAGTTGGTCGTTAAGCGTGCGCCGGACGGCCGAAG4540.15466217896526233No Hit
GTTATGAGTACGGCCTGGCGTGAAAACTGTTCCCTCCGGCGGATTTTCAA4470.15227751981822082No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA4330.14750820152413788No Hit
GTCCTGCTGTCTAGATGAACTAACGCCTTTTGTGGTGTCTGATGAGCGTG4330.14750820152413788No Hit
ACCCGGGGTCACCCCCGAGGCTTCGTCACCGACCCCCGCGCCTGCCTACT4240.14444221119222736No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA4240.14444221119222736No Hit
TTCCTGGACGGGCGGCGGGGTATTGGTAGCACGCTTGAGCGCCATCCATT4200.14307954882248938No Hit
GGCCAGCATCGGTTCGGGCGGCCGGACAAAGGCCCCGGGAACGTAGCACC4200.14307954882248938No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT4180.1423982176376204No Hit
GTATTGGTAGCACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCG4180.1423982176376204No Hit
CTATAACGCACCCCGAGGGGTGCTACGTTCCCGGGGCCTTTGTCCGGCCG4160.1417168864527514No Hit
GTCCGGGCTGGCCGCATTGCGCCCCGGGCTATAACGCACCCCGAGGGGTG4130.14069488967544788No Hit
CTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTT4120.14035422408301337No Hit
TTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCT4110.1400135584905789No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGGCCTGGCGTGAAAACTGTTC4100.13967289289814439No Hit
GAGGTGGGAACCCCCAAGGGTGCACCATCGACCGATCCTGATGTCTTCGG4080.1389915617132754No Hit
GCTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACAC4070.1386508961208409No Hit
GCCGAAGCCTGCCGAGGGCGTCCGCCCGCCGGTGCTGGAGGGCGCCGGCC4030.1372882337511029No Hit
GTCGTTAAGCGTGCGCCGGACGGCCGAAGCCTGCCGAGGGCGTCCGCCCG3990.1359255713813649No Hit
GGGCTATAACGCACCCCGAGGGGTGCTACGTTCCCGGGGCCTTTGTCCGG3930.1338815778267579No Hit
CTAGTAACGGTACATTCGATTGTCCGCGTTCAACTAAGAAACAAGGACTT3930.1338815778267579No Hit
AGCCCGGACCGAGGACCGCGCTTCGGCTAGGATGCTGGCGTAATGGTCGT3910.13320024664188893No Hit
GCGTTATAGCCCGGGGCGCAATGCGGCCAGCCCGGACCGAGGACCGCGCT3900.13285958104945442No Hit
GGTTAGTCGGTCCTAAGGGGTAGGGGAATTCCGATTCAACGTGCGCGCTC3890.13251891545701994No Hit
GTCGTAGAGGGTGAGAACCCCGTATGCGGCCGGCGGCCGAGCCCGTGTGA3880.13217824986458543No Hit
TTTCAAGACGGGTCGCTTACGACCATTACGCCAGCATCCTAGCCGAAGCG3870.13183758427215092No Hit
GATTTGAGTAAGAGCGTAGCTGTTGGGACCCGAAAGATGGTGAACTATGC3860.13149691867971644No Hit
TATTTAAAGTTTGAGAATAGGTTAAGGTTGTTTCAACCCCAAGGCCTCTA3860.13149691867971644No Hit
GAGTACGGCCTGGCGTGAAAACTGTTCCCTCCGGCGGATTTTCAAGGGTC3850.13115625308728193No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTCGTTAAGCGTGCG3800.12945292512510945No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAG3770.12843092834780592No Hit
GGGTATTGGTAGCACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATT3730.12706826597806795No Hit
TTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACACGGGCTCGGCCGCC3700.12604626920076445No Hit
CAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCTCCC3660.12468360683102646No Hit
CCCCGTATGCGGCCGGCGGCCGAGCCCGTGTGAAGCTCCTTCGACGAGTC3650.12434294123859196No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTT3620.12332094446128845No Hit
CATCAGACACCACAAAAGGCGTTAGTTCATCTAGACAGCAGGACGGTGGC3600.12263961327641947No Hit
GGTGAGAACCCCGTATGCGGCCGGCGGCCGAGCCCGTGTGAAGCTCCTTC3580.12195828209155048No Hit
GCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACT3570.12161761649911597No Hit
ACTCAAATCCATCCGAAGACATCAGGATCGGTCGATGGTGCACCCTTGGG3560.12127695090668147No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAAT3530.12025495412937798No Hit
CTTTAAATATGTAAGAAGTCCTTGTTTCTTAGTTGAACGCGGACAATCGA3500.11923295735207448No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT3460.11787029498233649No Hit
ATGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGCAGCG3460.11787029498233649No Hit
ATGGTGAACTATGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAG3440.1171889637974675No Hit
ATGGTGCACCCTTGGGGGTTCCCACCTCCGTTCACTTTCATTGCGCGCAC3400.1158263014277295No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGGTAAGGGAAG3400.1158263014277295No Hit
GGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCT3330.11344164228068801No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3310.11276031109581902No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGC3290.11207897991095002No Hit
ATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGG3290.11207897991095002No Hit
GTCTTGAAACACGGACCAAGGAGTCTAACATCTACGCGAGTGTTTGGGTG3280.11173831431851551No Hit
CTACTCGCCTGTCCATCGTTCCTGGACGGGCGGCGGGGTATTGGTAGCAC3270.111397648726081No Hit
TGATAGAACAATGTAGGTAAGGGAAGTCGGCAAAATAGATCCGTAACTTC3240.11037565194877753No Hit
GTTCACCATCTTTCGGGTCCCAACAGCTACGCTCTTACTCAAATCCATCC3230.11003498635634303No Hit
TCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGA3150.10730966161686703No Hit
CCCTTACCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT3110.10594699924712904No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGAC3100.10560633365469453No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAA3090.10526566806226005No Hit
AGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGA3090.10526566806226005No Hit
GATTAGAGGCCTTGGGGTTGAAACAACCTTAACCTATTCTCAAACTTTAA3060.10424367128495655No Hit
CCGTTACTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGA3060.10424367128495655No Hit
CCCCTATACCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCGAG3040.10356234010008755No Hit
GTTCTCACCCTCTACGACGCCCTGTTCCAAGGAACTTAGACCGGGGCCTC3030.10322167450765304No Hit
GTCTAAGTTCCTTGGAACAGGGCGTCGTAGAGGGTGAGAACCCCGTATGC3030.10322167450765304No Hit
TCCCTTACCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACC3000.10219967773034955No Hit
TTCCGGCACCTTAACCCCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGC2990.10185901213791507No Hit
GGTAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGC2980.10151834654548056No Hit
GACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAA2980.10151834654548056No Hit
CCTCTAATCATTCGCTTTACCTCATAAAACTGAAGGTCGTTACTGCTATC2980.10151834654548056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.10117768095304608No Hit
CCCTATCTCCGGACAAACCGATTTCAGGGTGACGGGCCGTCAAGAAGAAA2950.10049634976817706No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTTG250.002349900335.232
TTAGGAC502.3534176E-630.8000013
TAGGACC502.3534176E-630.8000014
TATTAGA300.0057380229.33333217
GAGAGTC4300.027.62790739
TTGGAAA4700.027.6170232
GTCCTCA407.019367E-427.56
AGAGAGT4450.027.19101138
AGAGTCA4500.026.8888940
GTCAAAA4500.026.8888943
TTTGGAA4800.026.58333231
TCAAAAA4750.026.444
AAGAGAG4750.026.437
CTTTGGA4850.026.30927830
CTCTACT2850.026.2456151
AGTCAAA4550.026.1098942
TACTTGT2950.026.1016944
GAGTCAA4700.025.7446841
CCTTAGG609.720185E-625.6666661
CTACTTG3050.025.2459013
Produced by FastQC (version 0.11.5)