FastQCFastQC Report
Thu 2 Feb 2017
SRR4062287_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062287_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062787
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81970.26763206190962674No Hit
TATCAACGCAGAGTACTTTTTTTTT48170.15727505699874003No Hit
GATTAAGAGGGACGGCCGGGGGCAT34780.11355670505327337No Hit
GAATAGGACCGCGGTTCTATTTTGT34320.11205480498643883No Hit
GCGCAAGACGGACCAGAGCGAAAGC33690.10999785489490455No Hit
GGTATCAACGCAGAGTACTTTTTTT32420.10585130471038307No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG31950.10431675464209558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57950.012.5355671
GGTATCA20750.012.4476351
TAATCGG550.003067516712.09201215
CGCATCG5300.012.01033413
GCATCGC5750.011.07057614
CGCCAGT6050.010.98968318
ATCGCCA5900.010.94925716
GGTTCTA7400.010.78459213
CGGTTCT7250.010.74563212
GGACCGT2300.010.7406386
TCGCGTA3000.010.4514679
TAACGAA2100.010.40554113
CGTCGTA6100.010.2801310
GTTCAAA8050.010.2626471
GTCTTAG3000.010.1289571
CCGATCA856.588331E-410.0602349
CGGACCA6550.010.0090389
GCGTTAT3250.09.934171
AACCGCG2201.8189894E-129.9333737
TTAATAC2201.8189894E-129.9307763
Produced by FastQC (version 0.11.5)