##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062287_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3062787
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.207236742222037	32.0	32.0	32.0	32.0	32.0
2	30.799225346065526	32.0	32.0	32.0	32.0	32.0
3	30.817255656367877	32.0	32.0	32.0	32.0	32.0
4	30.81316134618568	32.0	32.0	32.0	32.0	32.0
5	30.770608925792096	32.0	32.0	32.0	32.0	32.0
6	34.40523320753288	36.0	36.0	36.0	32.0	36.0
7	34.33273453230669	36.0	36.0	36.0	32.0	36.0
8	34.3009797938936	36.0	36.0	36.0	32.0	36.0
9	34.40134981636007	36.0	36.0	36.0	32.0	36.0
10	34.135874287046406	36.0	36.0	36.0	32.0	36.0
11	34.392139250950194	36.0	36.0	36.0	32.0	36.0
12	34.236354666517784	36.0	36.0	36.0	32.0	36.0
13	34.31426083498461	36.0	36.0	36.0	32.0	36.0
14	34.223830452460454	36.0	36.0	36.0	32.0	36.0
15	34.191974499042864	36.0	36.0	36.0	32.0	36.0
16	34.191269259011484	36.0	36.0	36.0	32.0	36.0
17	34.12616385011429	36.0	36.0	36.0	32.0	36.0
18	34.13598562355136	36.0	36.0	36.0	32.0	36.0
19	34.13225013688513	36.0	36.0	36.0	32.0	36.0
20	34.11157909446527	36.0	36.0	36.0	32.0	36.0
21	34.09035234902068	36.0	36.0	36.0	32.0	36.0
22	34.067432048000725	36.0	36.0	36.0	32.0	36.0
23	34.049041934682364	36.0	36.0	36.0	32.0	36.0
24	34.02684972869481	36.0	36.0	36.0	32.0	36.0
25	33.681901157344605	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	50.0
5	259.0
6	641.0
7	113.0
8	527.0
9	420.0
10	265.0
11	74.0
12	140.0
13	135.0
14	518.0
15	819.0
16	1247.0
17	1741.0
18	2345.0
19	3321.0
20	4904.0
21	7281.0
22	10302.0
23	14865.0
24	20399.0
25	27919.0
26	37032.0
27	46063.0
28	59374.0
29	77463.0
30	99483.0
31	133689.0
32	188411.0
33	269299.0
34	607625.0
35	1446060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.75649848355385	17.625613296272995	12.350457252021302	25.267430968151857
2	16.893203629902487	19.657814366154692	36.76445140398501	26.684530599957817
3	19.228751200301165	23.28850358808831	27.921886473467016	29.56085873814351
4	13.492718632060225	15.758141946674487	34.31836482530137	36.43077459596392
5	15.654056342113137	35.73424645722476	32.175303848420036	16.43639335224207
6	34.587201882910115	34.229549587459985	16.54505334236803	14.638195187261868
7	31.02155521243396	30.180271757533607	19.585800518517456	19.21237251151498
8	28.79606975980231	31.99066469893164	18.782521152334397	20.430744388931654
9	27.403946423002516	14.897710875905585	18.583401382248354	39.11494131884354
10	17.48676186527901	26.126009779676114	29.306954712435935	27.080273642608944
11	36.769270458171086	21.706618397403346	21.54810408975415	19.976007054671417
12	25.319644847413663	23.508964310480284	27.171553006265224	23.99983783584083
13	29.128821054207698	19.71518980099771	24.792819688970447	26.36316945582415
14	24.44157727370199	19.460654455875353	24.349329487387354	31.748438783035297
15	25.573221762794034	26.263105119182327	21.988744645075105	26.17492847294854
16	26.18901448360044	25.781632574338538	22.745293794209047	25.284059147851977
17	24.28690800696468	25.81290035313787	24.27762350835049	25.622568131546963
18	25.04701283410329	24.986900474001814	25.237352297328354	24.728734394566548
19	25.936035720693823	24.67922917608332	24.884410075499904	24.50032502772295
20	26.47789815559513	23.787224627708785	24.325509664848852	25.409367551847232
21	27.30984921272966	23.737230141767483	24.10432884304921	24.848591802453644
22	26.759545576218674	23.605451804167263	24.42807271904827	25.20692990056579
23	24.95035508734139	23.91899571045734	25.063418373698997	26.067230828502275
24	24.9973691933522	24.551373934686712	24.977270484179538	25.473986387781554
25	25.20639091232921	24.038895806168036	24.931094095693748	25.82361918580901
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	674.0
1	674.0
2	1034.0
3	1394.0
4	1394.0
5	1394.0
6	2514.5
7	3635.0
8	3635.0
9	3635.0
10	4358.5
11	5082.0
12	5082.0
13	5082.0
14	6195.0
15	7308.0
16	7308.0
17	7308.0
18	12018.0
19	16728.0
20	16728.0
21	16728.0
22	25992.0
23	35256.0
24	35256.0
25	35256.0
26	52386.0
27	69516.0
28	69516.0
29	69516.0
30	89156.5
31	108797.0
32	108797.0
33	108797.0
34	137163.0
35	165529.0
36	165529.0
37	165529.0
38	199498.5
39	233468.0
40	233468.0
41	233468.0
42	272436.0
43	311404.0
44	311404.0
45	311404.0
46	353114.5
47	394825.0
48	394825.0
49	394825.0
50	413993.5
51	433162.0
52	433162.0
53	433162.0
54	407656.0
55	382150.0
56	382150.0
57	382150.0
58	353548.0
59	324946.0
60	324946.0
61	324946.0
62	287229.0
63	249512.0
64	249512.0
65	249512.0
66	203219.0
67	156926.0
68	156926.0
69	156926.0
70	119342.0
71	81758.0
72	81758.0
73	81758.0
74	61166.0
75	40574.0
76	40574.0
77	40574.0
78	32092.0
79	23610.0
80	23610.0
81	23610.0
82	16337.5
83	9065.0
84	9065.0
85	9065.0
86	6694.0
87	4323.0
88	4323.0
89	4323.0
90	2926.0
91	1529.0
92	1529.0
93	1529.0
94	940.0
95	351.0
96	351.0
97	351.0
98	808.0
99	1265.0
100	1265.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011101000493994521
2	1.6325000726462533E-4
3	4.571000203409509E-4
4	0.010219450454765544
5	0.034706951544459345
6	0.06507145289567964
7	0.10758175478738807
8	0.11205480498643883
9	0.12122945539471076
10	0.13709735610083235
11	0.1432682063754352
12	0.13575870604126242
13	0.1298817057797359
14	0.11868275528138261
15	0.13562810603545072
16	0.12214365543539267
17	0.1281512557027309
18	0.11479740510848453
19	0.0995172044285156
20	0.09416260419023588
21	0.09295455413647766
22	0.10369640461449
23	0.08338810371077061
24	0.09432585419750052
25	0.0919750540928899
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3062787.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.31497937483521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.08611274707073	24.937730674922147
2	16.44684392518792	12.603209715494183
3	7.111870766567882	8.174735452126257
4	3.564107331473951	5.462347955804935
5	1.9999680427368576	3.8314367153896125
6	1.2462055689199618	2.8649004401983875
7	0.8701911475137545	2.3338949108407583
8	0.6322124781686523	1.9378566449236296
9	0.4448333534045259	1.5339402684839873
>10	2.331390048782832	16.032920392362282
>50	0.1513460214381741	3.9711980350553753
>100	0.09332930077472705	7.324871554825096
>500	0.013707471720806932	3.6580612465623394
>1k	0.007796124541133009	5.063828701459181
>5k	8.567169825420889E-5	0.2690672915520553
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8197	0.26763206190962674	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4817	0.15727505699874003	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3478	0.11355670505327337	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3432	0.11205480498643883	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3369	0.10999785489490455	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3242	0.10585130471038307	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3195	0.10431675464209558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9590000871755038E-4
2	0.0	0.0	0.0	0.0	1.9590000871755038E-4
3	0.0	0.0	0.0	0.0	1.9590000871755038E-4
4	0.0	0.0	0.0	0.0	1.9590000871755038E-4
5	0.0	1.6325000726462533E-4	0.0	0.0	2.2855001017047546E-4
6	0.0	1.6325000726462533E-4	0.0	0.0	2.9385001307632557E-4
7	0.0	1.6325000726462533E-4	0.0	0.0	2.9385001307632557E-4
8	0.0	1.6325000726462533E-4	0.0	0.0	2.9385001307632557E-4
9	0.0	1.6325000726462533E-4	0.0	0.0	2.9385001307632557E-4
10	0.0	1.9590000871755038E-4	0.0	0.0	2.9385001307632557E-4
11	0.0	1.9590000871755038E-4	0.0	0.0	2.9385001307632557E-4
12	0.0	1.9590000871755038E-4	0.0	0.0	5.550500246997261E-4
13	0.0	1.9590000871755038E-4	0.0	0.0	5.877000261526511E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5795	0.0	12.535567	1
GGTATCA	2075	0.0	12.447635	1
TAATCGG	55	0.0030675167	12.092012	15
CGCATCG	530	0.0	12.010334	13
GCATCGC	575	0.0	11.070576	14
CGCCAGT	605	0.0	10.989683	18
ATCGCCA	590	0.0	10.949257	16
GGTTCTA	740	0.0	10.784592	13
CGGTTCT	725	0.0	10.745632	12
GGACCGT	230	0.0	10.740638	6
TCGCGTA	300	0.0	10.451467	9
TAACGAA	210	0.0	10.405541	13
CGTCGTA	610	0.0	10.28013	10
GTTCAAA	805	0.0	10.262647	1
GTCTTAG	300	0.0	10.128957	1
CCGATCA	85	6.588331E-4	10.060234	9
CGGACCA	655	0.0	10.009038	9
GCGTTAT	325	0.0	9.93417	1
AACCGCG	220	1.8189894E-12	9.933373	7
TTAATAC	220	1.8189894E-12	9.930776	3
>>END_MODULE