FastQCFastQC Report
Thu 2 Feb 2017
SRR4062287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062787
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA36200.11819300525958874No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT34820.11368730505908507No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT32190.10510035467696578No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA31610.10320665459269612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60050.025.7654611
GGTATCA25950.025.528471
TCAACGC88700.017.3363514
ATCAACG88850.017.3070853
TATCAAC90950.017.222762
AACGCAG89850.017.1634316
CAACGCA91600.016.7874955
CGTAATC1105.627812E-515.99931713
CGTCGTA10200.015.09739510
ATACCGT9800.014.8156946
TACCGTC10200.014.4503647
ACGCAGA107400.014.3997587
CGCAGAG109300.014.169578
ACCGTCG10450.013.8941448
AGAGTAC111450.013.87648311
TGTACGC1751.0540462E-613.8286592
CAGAGTA112000.013.82798210
CCTACAC4950.013.7771913
GTCCTAT7600.013.61068251
GTATAGG4900.013.4747511
Produced by FastQC (version 0.11.5)