##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062287_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3062787
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.295730653160014	32.0	32.0	32.0	32.0	32.0
2	31.483734912026204	32.0	32.0	32.0	32.0	32.0
3	31.57854496574525	32.0	32.0	32.0	32.0	32.0
4	31.654209385112317	32.0	32.0	32.0	32.0	32.0
5	31.63608471630577	32.0	32.0	32.0	32.0	32.0
6	35.2678697539202	36.0	36.0	36.0	36.0	36.0
7	35.26517025180008	36.0	36.0	36.0	36.0	36.0
8	35.22937801420732	36.0	36.0	36.0	36.0	36.0
9	35.290634967433256	36.0	36.0	36.0	36.0	36.0
10	35.18451103521074	36.0	36.0	36.0	36.0	36.0
11	35.2987955087964	36.0	36.0	36.0	36.0	36.0
12	35.22158968286074	36.0	36.0	36.0	36.0	36.0
13	35.255370027363966	36.0	36.0	36.0	36.0	36.0
14	35.212902823474174	36.0	36.0	36.0	36.0	36.0
15	35.19984837339325	36.0	36.0	36.0	36.0	36.0
16	35.20700427421169	36.0	36.0	36.0	36.0	36.0
17	35.189407229428625	36.0	36.0	36.0	36.0	36.0
18	35.186639488805454	36.0	36.0	36.0	36.0	36.0
19	35.17385244223643	36.0	36.0	36.0	36.0	36.0
20	35.17626854234395	36.0	36.0	36.0	36.0	36.0
21	35.162763522242976	36.0	36.0	36.0	36.0	36.0
22	35.15075550470862	36.0	36.0	36.0	36.0	36.0
23	35.12148020740587	36.0	36.0	36.0	36.0	36.0
24	35.10796082130425	36.0	36.0	36.0	36.0	36.0
25	35.08967910599072	36.0	36.0	36.0	36.0	36.0
26	35.04860083316274	36.0	36.0	36.0	36.0	36.0
27	35.03763500367476	36.0	36.0	36.0	36.0	36.0
28	35.01519465767616	36.0	36.0	36.0	36.0	36.0
29	34.99890916345146	36.0	36.0	36.0	36.0	36.0
30	34.982942333240935	36.0	36.0	36.0	36.0	36.0
31	34.982480335720375	36.0	36.0	36.0	36.0	36.0
32	34.96000178922008	36.0	36.0	36.0	36.0	36.0
33	34.92927226085261	36.0	36.0	36.0	32.0	36.0
34	34.922466368049754	36.0	36.0	36.0	36.0	36.0
35	34.90695141385934	36.0	36.0	36.0	36.0	36.0
36	34.88824296302681	36.0	36.0	36.0	32.0	36.0
37	34.87940068963333	36.0	36.0	36.0	32.0	36.0
38	34.86155713733929	36.0	36.0	36.0	32.0	36.0
39	34.84232857198362	36.0	36.0	36.0	32.0	36.0
40	34.82598789925646	36.0	36.0	36.0	32.0	36.0
41	34.801629365672504	36.0	36.0	36.0	32.0	36.0
42	34.76589459208231	36.0	36.0	36.0	32.0	36.0
43	34.756641255170535	36.0	36.0	36.0	32.0	36.0
44	34.708252320517225	36.0	36.0	36.0	32.0	36.0
45	34.685991549526626	36.0	36.0	36.0	32.0	36.0
46	34.663020641004415	36.0	36.0	36.0	32.0	36.0
47	34.62920536099964	36.0	36.0	36.0	32.0	36.0
48	34.59906353265833	36.0	36.0	36.0	32.0	36.0
49	34.58608744258089	36.0	36.0	36.0	32.0	36.0
50	34.17926222097717	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	13.0
20	45.0
21	128.0
22	281.0
23	774.0
24	1904.0
25	4116.0
26	8519.0
27	15504.0
28	26203.0
29	40372.0
30	58855.0
31	84592.0
32	124855.0
33	205913.0
34	466364.0
35	2024346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.35015350447449	18.17662812724541	12.77124567248024	25.701972695799856
2	16.352621419074413	20.04531420197635	36.48296097192727	27.11910340702197
3	18.70187995688888	23.559716767397497	28.196153628905474	29.54224964680815
4	13.189163986917798	16.05207936431753	34.41584413150506	36.342912517259606
5	15.271254579570828	35.94232964943367	32.10330982859729	16.683105942398214
6	34.69541743421504	34.28170859042531	16.44704063168714	14.575833343672517
7	30.995070829280653	30.146856441535114	19.659741274858487	19.19833145432575
8	28.92528928717537	31.885338418897557	18.76646988510791	20.42290240881916
9	27.551148676026116	14.592265149355798	18.575140876593768	39.281445298024316
10	17.521269850391114	26.007791598890684	29.457363328681797	27.01357522203641
11	37.0387166982229	21.46995530541301	21.565228009652646	19.92609998671145
12	25.38695161855905	23.28468263861726	27.285190672805737	24.04317507001795
13	29.342229805729225	19.41421326393249	24.937614009723823	26.305942920614463
14	24.53128474164217	19.187752853855002	24.388343035281267	31.892619369221563
15	25.694898143422968	26.110206161904177	22.038293880704078	26.156601813968784
16	26.247523074707885	25.567354247088176	22.868979018659257	25.316143659544682
17	24.45001888802584	25.642168391076492	24.312790931919196	25.595021788978467
18	25.269778975670835	24.665165853953184	25.38536005628864	24.67969511408734
19	26.048922109177035	24.369699884451645	25.1042269671381	24.47715103923322
20	26.587701291997423	23.497140013784875	24.54076806767192	25.37439062654578
21	27.444303142401445	23.48503078080897	24.289396741854226	24.78126933493536
22	26.82070284352128	23.406067741570013	24.655909797187984	25.117319617720725
23	25.103272055750587	23.598268764627214	25.28075763749582	26.01770154212638
24	25.133670284318722	24.191676788256345	25.164002403061293	25.510650524363648
25	25.437307384768793	23.63413986718589	25.18195088966647	25.74660185837885
26	25.08232732810191	24.65604709978601	25.352507236903538	24.90911833520854
27	25.64487333696403	24.066662487446013	24.707650455208547	25.580813720381407
28	25.288834224745592	23.883362071189126	25.3578573194029	25.46994638466238
29	25.088432904288727	24.180726361135363	25.249887846768974	25.480952887806936
30	25.086523718600052	24.35727569787093	25.365423946630866	25.190776636898153
31	25.57798150759557	24.016682931894024	24.58835479374723	25.81698076676317
32	25.57931408272277	24.09970092335018	24.37562851806867	25.94535647585838
33	24.96333396456814	23.877065933563628	24.988441873069565	26.171158228798674
34	25.707016834146163	24.0440739293246	25.02831593564373	25.220593300885508
35	26.094578190126743	23.69303292631722	25.184162874357853	25.028226009198185
36	25.104178196221017	24.417743220948545	24.656839649978238	25.821238932852204
37	26.01397249709491	24.185898141234084	24.673763383072167	25.12636597859884
38	25.27900242491561	24.05172152030161	24.80495705382059	25.864319000962194
39	25.999114524732903	23.95151827011044	24.28562846215279	25.763738743003863
40	25.89927987086259	24.20660418756269	24.746635740554996	25.14748020101973
41	25.0541667591757	24.687047385955953	24.97521846280027	25.28356739206808
42	26.3097953233082	24.52629843255889	24.575373820256086	24.588532423876824
43	25.950605569746234	23.6901144204061	24.641838301629342	25.71744170821833
44	25.29404946345748	24.35330014872668	24.464510483516808	25.888139904299035
45	25.453503596488076	24.521192016220926	24.48994517926934	25.535359208021653
46	25.376340926269663	24.408768037509205	24.42179569434309	25.793095341878043
47	25.546403304736	24.317281559484332	24.8902948847378	25.246020251041873
48	26.145776806080423	24.91300108858837	23.753592439567672	25.187629665763538
49	25.41343092157677	24.989127112866445	23.894328597586938	25.70311336796984
50	25.660276080129712	25.032732192870817	23.955949161762007	25.351042565237464
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	243.0
1	175.5
2	108.0
3	258.5
4	409.0
5	481.0
6	553.0
7	518.5
8	484.0
9	669.0
10	854.0
11	1271.5
12	1689.0
13	3117.0
14	4545.0
15	6239.0
16	7933.0
17	8059.5
18	8186.0
19	7811.0
20	7436.0
21	7650.0
22	7864.0
23	8831.5
24	9799.0
25	11277.0
26	12755.0
27	15873.5
28	18992.0
29	22636.0
30	26280.0
31	31425.5
32	36571.0
33	42486.0
34	48401.0
35	56203.5
36	64006.0
37	77937.0
38	91868.0
39	104299.0
40	116730.0
41	129459.5
42	142189.0
43	149790.5
44	157392.0
45	176052.0
46	194712.0
47	209491.0
48	224270.0
49	234106.0
50	243942.0
51	240688.5
52	237435.0
53	240102.5
54	242770.0
55	244502.5
56	246235.0
57	239516.0
58	232797.0
59	214290.0
60	195783.0
61	173178.5
62	150574.0
63	129727.5
64	108881.0
65	91720.0
66	74559.0
67	63304.5
68	52050.0
69	45090.5
70	38131.0
71	29362.5
72	20594.0
73	17372.0
74	14150.0
75	10407.0
76	6664.0
77	5959.5
78	5255.0
79	4381.5
80	3508.0
81	2843.0
82	2178.0
83	1902.5
84	1627.0
85	1230.0
86	833.0
87	561.5
88	290.0
89	194.5
90	99.0
91	75.5
92	52.0
93	43.0
94	34.0
95	32.5
96	31.0
97	31.0
98	31.0
99	23.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03222555143403704
2	0.005583150248450186
3	1.3060000581170027E-4
4	0.0
5	0.0
6	1.6325000726462533E-4
7	0.0
8	0.0
9	0.0
10	3.265000145292507E-5
11	0.0
12	3.265000145292507E-5
13	0.0
14	0.0
15	0.0
16	5.877000261526511E-4
17	0.0
18	6.530000290585014E-5
19	0.0
20	3.2650001452925065E-4
21	6.530000290585014E-5
22	0.0
23	9.795000435877519E-5
24	8.815500392289768E-4
25	3.2650001452925065E-4
26	4.2445001888802584E-4
27	4.897500217938759E-4
28	0.001436600063928703
29	4.2445001888802584E-4
30	0.0014039500624757778
31	5.224000232468011E-4
32	8.815500392289768E-4
33	2.2855001017047546E-4
34	7.836000348702015E-4
35	2.9385001307632557E-4
36	1.9590000871755038E-4
37	0.0018284000813638037
38	0.0
39	8.162500363231265E-4
40	9.795000435877519E-5
41	7.509500334172764E-4
42	0.00496280022084461
43	0.0043751001946919586
44	0.003983300177256858
45	0.00251405011187523
46	0.0023508001046106046
47	0.001012150045040677
48	0.0034282501525571317
49	0.004015950178709783
50	0.0017304500770050284
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3062787.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.39382317394538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.32746438083768	25.341047570469893
2	17.06231261295103	13.442994520263413
3	7.2264056959846945	8.540272445124383
4	3.630197029918075	5.720293595326975
5	2.0516324877943664	4.041082372104646
6	1.2666326780765043	2.9938502247892016
7	0.8461855256623969	2.3334138079207545
8	0.5926954865657108	1.86788329550121
9	0.44484146404023106	1.577160537735586
>10	2.3051054693631947	16.133895223990315
>50	0.14217438596574528	3.8262410640140354
>100	0.08594178366543737	6.790970387787853
>500	0.011912999466296204	3.2181084526234276
>1k	0.006497999708702629	4.172786502348114
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3620	0.11819300525958874	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	3482	0.11368730505908507	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3219	0.10510035467696578	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	3161	0.10320665459269612	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2855001017047546E-4
2	0.0	6.530000290585012E-5	0.0	0.0	2.2855001017047546E-4
3	0.0	6.530000290585012E-5	0.0	0.0	2.2855001017047546E-4
4	0.0	6.530000290585012E-5	0.0	0.0	2.2855001017047546E-4
5	0.0	6.530000290585012E-5	0.0	0.0	2.2855001017047546E-4
6	0.0	6.530000290585012E-5	0.0	0.0	2.9385001307632557E-4
7	0.0	6.530000290585012E-5	0.0	0.0	2.9385001307632557E-4
8	0.0	6.530000290585012E-5	0.0	0.0	2.9385001307632557E-4
9	0.0	6.530000290585012E-5	0.0	0.0	2.9385001307632557E-4
10	0.0	1.6325000726462533E-4	0.0	0.0	2.9385001307632557E-4
11	0.0	1.6325000726462533E-4	0.0	0.0	3.2650001452925065E-4
12	0.0	1.9590000871755038E-4	0.0	0.0	9.142000406819019E-4
13	0.0	1.9590000871755038E-4	0.0	0.0	9.79500043587752E-4
14	0.0	1.9590000871755038E-4	0.0	0.0	0.001012150045040677
15	0.0	1.9590000871755038E-4	0.0	0.0	0.0011754000523053023
16	0.0	2.2855001017047546E-4	0.0	3.265000145292506E-5	0.0013386500595699276
17	0.0	2.2855001017047546E-4	0.0	3.265000145292506E-5	0.0013713000610228528
18	0.0	2.2855001017047546E-4	0.0	9.795000435877519E-5	0.001436600063928703
19	0.0	2.2855001017047546E-4	0.0	1.9590000871755038E-4	0.001436600063928703
20	0.0	2.2855001017047546E-4	0.0	1.9590000871755038E-4	0.001436600063928703
21	0.0	2.2855001017047546E-4	0.0	1.9590000871755038E-4	0.0015019000668345529
22	0.0	2.2855001017047546E-4	0.0	2.612000116234005E-4	0.0015019000668345529
23	0.0	2.2855001017047546E-4	0.0	4.89750021793876E-4	0.0015019000668345529
24	0.0	2.2855001017047546E-4	0.0	6.856500305114264E-4	0.0015019000668345529
25	0.0	2.2855001017047546E-4	0.0	9.79500043587752E-4	0.0015019000668345529
26	0.0	2.2855001017047546E-4	0.0	0.0011427500508523773	0.0016651500740991782
27	0.0	2.2855001017047546E-4	0.0	0.0013386500595699276	0.0017304500770050284
28	0.0	2.2855001017047546E-4	0.0	0.0021222500944401293	0.0017304500770050284
29	0.0	2.2855001017047546E-4	0.0	0.003362950149651282	0.0017304500770050284
30	0.0	2.612000116234005E-4	0.0	0.005713750254261886	0.0017304500770050284
31	0.0	2.612000116234005E-4	0.0	0.011525450512882549	0.0017304500770050284
32	0.0	2.612000116234005E-4	0.0	0.016945350754068108	0.0018610500828167287
33	0.0	3.2650001452925065E-4	0.0	0.022920301019953394	0.0018610500828167287
34	0.0	3.2650001452925065E-4	0.0	0.028634051274215283	0.0018937000842696537
35	0.0	3.2650001452925065E-4	0.0	0.03539260157497077	0.0018937000842696537
36	0.0	3.2650001452925065E-4	0.0	0.04587325204135972	0.0018937000842696537
37	0.0	3.2650001452925065E-4	0.0	0.0613493527300462	0.0018937000842696537
38	0.0	3.2650001452925065E-4	0.0	0.08103730360616002	0.001959000087175504
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6005	0.0	25.765461	1
GGTATCA	2595	0.0	25.52847	1
TCAACGC	8870	0.0	17.336351	4
ATCAACG	8885	0.0	17.307085	3
TATCAAC	9095	0.0	17.22276	2
AACGCAG	8985	0.0	17.163431	6
CAACGCA	9160	0.0	16.787495	5
CGTAATC	110	5.627812E-5	15.999317	13
CGTCGTA	1020	0.0	15.097395	10
ATACCGT	980	0.0	14.815694	6
TACCGTC	1020	0.0	14.450364	7
ACGCAGA	10740	0.0	14.399758	7
CGCAGAG	10930	0.0	14.16957	8
ACCGTCG	1045	0.0	13.894144	8
AGAGTAC	11145	0.0	13.876483	11
TGTACGC	175	1.0540462E-6	13.828659	2
CAGAGTA	11200	0.0	13.827982	10
CCTACAC	495	0.0	13.777191	3
GTCCTAT	760	0.0	13.6106825	1
GTATAGG	490	0.0	13.474751	1
>>END_MODULE