FastQCFastQC Report
Thu 2 Feb 2017
SRR4062286_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062286_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896066
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16110.17978586398769733No Hit
TATCAACGCAGAGTACTTTTTTTTT10640.11874125343445682No Hit
GCGCAAGACGGACCAGAGCGAAAGC9830.1097017407199916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAC456.762437E-414.7766043
CATTCGG500.001497685613.30117310
TAATACT1001.0053554E-813.2996864
GGTATCA3950.013.2231051
CGTTATT655.45437E-513.1520662
CGGTCCA1352.7284841E-1112.66778410
TCGCGTA604.087898E-412.6670769
TATATAG604.089825E-412.6663695
GCGCCGA905.405236E-712.66495419
GTCGGCG853.9316983E-612.2958912
CGTCTTA550.003065956312.091315
GTATCAA9300.011.9473281
TCGGCGT802.8605964E-511.87604713
GACGATC658.011662E-411.6933388
CGCGTAA658.011662E-411.69333810
GCCAATA658.0266956E-411.6907262
ATATACG907.4432537E-611.6121356
AGGTTAT752.0702448E-411.4010066
CGCCGGT1354.7020876E-911.2608817
CGGCGTC855.31503E-511.17683214
Produced by FastQC (version 0.11.5)