##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062286_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	896066
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21478886599871	32.0	32.0	32.0	32.0	32.0
2	30.76686538714782	32.0	32.0	32.0	32.0	32.0
3	30.802133994594147	32.0	32.0	32.0	32.0	32.0
4	30.80487151616064	32.0	32.0	32.0	32.0	32.0
5	30.741698714157216	32.0	32.0	32.0	32.0	32.0
6	34.36593956248758	36.0	36.0	36.0	32.0	36.0
7	34.31045592623757	36.0	36.0	36.0	32.0	36.0
8	34.25489751870956	36.0	36.0	36.0	32.0	36.0
9	34.37801344990213	36.0	36.0	36.0	32.0	36.0
10	34.08384427039972	36.0	36.0	36.0	32.0	36.0
11	34.36639823405865	36.0	36.0	36.0	32.0	36.0
12	34.2004852321146	36.0	36.0	36.0	32.0	36.0
13	34.288741007916826	36.0	36.0	36.0	32.0	36.0
14	34.1979519365761	36.0	36.0	36.0	32.0	36.0
15	34.145616505927016	36.0	36.0	36.0	32.0	36.0
16	34.148701100142176	36.0	36.0	36.0	32.0	36.0
17	34.080105706499296	36.0	36.0	36.0	32.0	36.0
18	34.08504619079398	36.0	36.0	36.0	32.0	36.0
19	34.09728078065678	36.0	36.0	36.0	32.0	36.0
20	34.069512736785015	36.0	36.0	36.0	32.0	36.0
21	34.06234473799921	36.0	36.0	36.0	32.0	36.0
22	34.024686797624284	36.0	36.0	36.0	32.0	36.0
23	33.995490287545785	36.0	36.0	36.0	32.0	36.0
24	33.96704707019349	36.0	36.0	36.0	32.0	36.0
25	33.63183515499974	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	15.0
5	74.0
6	186.0
7	46.0
8	161.0
9	133.0
10	70.0
11	20.0
12	40.0
13	43.0
14	142.0
15	223.0
16	317.0
17	466.0
18	635.0
19	841.0
20	1305.0
21	1910.0
22	2812.0
23	4030.0
24	5678.0
25	7897.0
26	10885.0
27	13700.0
28	17973.0
29	23775.0
30	31011.0
31	42214.0
32	58616.0
33	82718.0
34	182272.0
35	405857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.22442693569627	17.487333437493024	11.390866683034618	25.897372943776087
2	16.855214098147336	19.19411736477762	37.130061457869495	26.820607079205544
3	19.379797380092004	22.422443982670842	28.10899245811116	30.088766179125997
4	13.125798012446763	15.229090957793511	34.759524808700235	36.88558622105949
5	15.148135620088707	36.036491632310636	33.29191631305909	15.523456434541572
6	35.41118531257678	34.434815625488575	16.08167869028209	14.072320371652559
7	31.144833996780154	29.942920856734588	19.75425468090312	19.157990465582138
8	28.361018751899326	33.23088164315976	18.755027618383654	19.653071986557265
9	26.683404620479745	14.625597225388058	18.728630616376826	39.96236753775537
10	16.78747038576729	26.55256805685933	30.67363998033168	25.986321577041704
11	37.47764803705061	21.357747637397516	21.4693956923297	19.69520863322217
12	24.244577947039996	23.733774070696775	28.287538665093244	23.73410931716998
13	29.459302111187966	19.361961517444986	25.053218296142916	26.12551807522413
14	23.39441340782123	19.406033519553073	24.795307262569832	32.40424581005586
15	25.251104098126163	27.166066192247172	21.754309111715063	25.828520597911602
16	25.87456422633414	25.92339322427818	23.178466076696168	25.023576472691516
17	23.987896097743032	26.255254671377713	24.882391459329234	24.87445777155002
18	24.866985915367064	25.305301122435527	25.55669271455993	24.27102024763748
19	25.572751890181777	25.18354424723399	25.089146871145907	24.154556991438326
20	25.715967725179205	24.556992002788245	24.64323096738447	25.08380930464808
21	26.316036402753777	24.443285184320498	24.536671406724924	24.7040070062008
22	25.8907803922182	24.32662208389099	24.893477255411042	24.88912026847977
23	24.340677445477645	24.4572896596018	25.563095138365306	25.638937756555247
24	24.682978704390486	24.861931528762028	25.270551648332006	25.184538118515476
25	24.83116146169605	24.509904672968187	25.2313920159422	25.427541849393563
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	273.0
1	273.0
2	293.0
3	313.0
4	313.0
5	313.0
6	656.0
7	999.0
8	999.0
9	999.0
10	1194.0
11	1389.0
12	1389.0
13	1389.0
14	1926.5
15	2464.0
16	2464.0
17	2464.0
18	4018.5
19	5573.0
20	5573.0
21	5573.0
22	8647.0
23	11721.0
24	11721.0
25	11721.0
26	17052.0
27	22383.0
28	22383.0
29	22383.0
30	28153.0
31	33923.0
32	33923.0
33	33923.0
34	43441.5
35	52960.0
36	52960.0
37	52960.0
38	62553.0
39	72146.0
40	72146.0
41	72146.0
42	83214.5
43	94283.0
44	94283.0
45	94283.0
46	104790.5
47	115298.0
48	115298.0
49	115298.0
50	119325.5
51	123353.0
52	123353.0
53	123353.0
54	115905.0
55	108457.0
56	108457.0
57	108457.0
58	99815.5
59	91174.0
60	91174.0
61	91174.0
62	80218.0
63	69262.0
64	69262.0
65	69262.0
66	56818.5
67	44375.0
68	44375.0
69	44375.0
70	33547.5
71	22720.0
72	22720.0
73	22720.0
74	17096.5
75	11473.0
76	11473.0
77	11473.0
78	9153.5
79	6834.0
80	6834.0
81	6834.0
82	4729.5
83	2625.0
84	2625.0
85	2625.0
86	1908.5
87	1192.0
88	1192.0
89	1192.0
90	811.0
91	430.0
92	430.0
93	430.0
94	262.0
95	94.0
96	94.0
97	94.0
98	223.0
99	352.0
100	352.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.695935344048318E-4
2	5.579946120040265E-4
3	4.463956896032212E-4
4	0.010043903016072477
5	0.03336807779784078
6	0.06762894697488801
7	0.11081772994399966
8	0.11271491162481335
9	0.122089221106481
10	0.1368202788633873
11	0.14396260989703882
12	0.13414190472576798
13	0.13023594244173978
14	0.11896445127925845
15	0.13592748748418085
16	0.12342840817529065
17	0.12811556291612447
18	0.11695567067604394
19	0.10200141507433604
20	0.09809545279030785
21	0.09586347434229175
22	0.10624217412556665
23	0.08827474761903699
24	0.09575187541989094
25	0.09285030343747
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	896066.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.94280446699871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.06732999110312	47.99460305851356
2	12.398597831387507	14.864134509436312
3	3.4006640115777995	6.1153601371190245
4	1.3800014502062272	3.3088462837554613
5	0.7234934318235835	2.1684112658479466
6	0.42696912840790746	1.5356236186560035
7	0.27760057767907165	1.1648110003419736
8	0.21948771012009288	1.0525367112510418
9	0.1500000511434312	0.8092281362157453
>10	0.8164658725113652	8.956761351123872
>50	0.07791915446437296	3.2882464363301325
>100	0.05717343958953933	6.8310890149336405
>500	0.003923667378518827	1.6107540475269626
>1k	3.7368260747798357E-4	0.2995944289483121
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1611	0.17978586398769733	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1064	0.11874125343445682	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	983	0.1097017407199916	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.115989224008053E-4
13	0.0	0.0	0.0	0.0	1.115989224008053E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAGAC	45	6.762437E-4	14.776604	3
CATTCGG	50	0.0014976856	13.301173	10
TAATACT	100	1.0053554E-8	13.299686	4
GGTATCA	395	0.0	13.223105	1
CGTTATT	65	5.45437E-5	13.152066	2
CGGTCCA	135	2.7284841E-11	12.667784	10
TCGCGTA	60	4.087898E-4	12.667076	9
TATATAG	60	4.089825E-4	12.666369	5
GCGCCGA	90	5.405236E-7	12.664954	19
GTCGGCG	85	3.9316983E-6	12.29589	12
CGTCTTA	55	0.0030659563	12.0913	15
GTATCAA	930	0.0	11.947328	1
TCGGCGT	80	2.8605964E-5	11.876047	13
GACGATC	65	8.011662E-4	11.693338	8
CGCGTAA	65	8.011662E-4	11.693338	10
GCCAATA	65	8.0266956E-4	11.690726	2
ATATACG	90	7.4432537E-6	11.612135	6
AGGTTAT	75	2.0702448E-4	11.401006	6
CGCCGGT	135	4.7020876E-9	11.260881	7
CGGCGTC	85	5.31503E-5	11.176832	14
>>END_MODULE