FastQCFastQC Report
Thu 2 Feb 2017
SRR4062286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896066
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10470.11684407175364316No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTG250.002352600735.19959614
TCGATCG454.0687E-529.33299841
GTATCAA11850.025.0727981
GTATACG500.00257424622.0083451
GTCTTAG1158.725692E-921.051461
GGTATCA5450.020.9987891
TATACCG550.00448301819.9997715
GTACTAG909.982108E-619.5629731
CAAGACG3950.019.4934484
CGTCGTA2150.019.44163910
CGCAAGA4000.018.7018742
AACCGCT600.007411130718.3331247
GCATTCG2400.018.33312422
ATACCGT2300.018.1737066
GGTATAT851.4261603E-418.1245211
AAGACGG4050.017.9257225
CCGTCGT2100.017.809329
GAGCGAA3850.017.71408316
ACCGTCG2250.017.59988
CGATCGT750.001290615617.599842
Produced by FastQC (version 0.11.5)