##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062286_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	896066
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.257391754625218	32.0	32.0	32.0	32.0	32.0
2	31.431722663285964	32.0	32.0	32.0	32.0	32.0
3	31.56133699972993	32.0	32.0	32.0	32.0	32.0
4	31.64783062854745	32.0	32.0	32.0	32.0	32.0
5	31.614013923081558	32.0	32.0	32.0	32.0	32.0
6	35.25812607553461	36.0	36.0	36.0	36.0	36.0
7	35.25712168523301	36.0	36.0	36.0	36.0	36.0
8	35.21210825988264	36.0	36.0	36.0	36.0	36.0
9	35.28602357415637	36.0	36.0	36.0	36.0	36.0
10	35.173547484225494	36.0	36.0	36.0	36.0	36.0
11	35.29974354567632	36.0	36.0	36.0	36.0	36.0
12	35.21337490765189	36.0	36.0	36.0	36.0	36.0
13	35.25616416647881	36.0	36.0	36.0	36.0	36.0
14	35.2161459088951	36.0	36.0	36.0	36.0	36.0
15	35.191774936221215	36.0	36.0	36.0	36.0	36.0
16	35.200105795778434	36.0	36.0	36.0	36.0	36.0
17	35.183967475610054	36.0	36.0	36.0	36.0	36.0
18	35.181246693881924	36.0	36.0	36.0	36.0	36.0
19	35.172735044070414	36.0	36.0	36.0	36.0	36.0
20	35.16899090022387	36.0	36.0	36.0	36.0	36.0
21	35.16854338854504	36.0	36.0	36.0	36.0	36.0
22	35.14671687130189	36.0	36.0	36.0	36.0	36.0
23	35.11885508433531	36.0	36.0	36.0	36.0	36.0
24	35.09356900049773	36.0	36.0	36.0	36.0	36.0
25	35.079480752533854	36.0	36.0	36.0	36.0	36.0
26	35.047581316554805	36.0	36.0	36.0	36.0	36.0
27	35.03328326261681	36.0	36.0	36.0	36.0	36.0
28	35.01896958482969	36.0	36.0	36.0	36.0	36.0
29	34.99610184964054	36.0	36.0	36.0	36.0	36.0
30	34.97879843672229	36.0	36.0	36.0	36.0	36.0
31	34.98009186823292	36.0	36.0	36.0	36.0	36.0
32	34.94502079087924	36.0	36.0	36.0	36.0	36.0
33	34.92685137032317	36.0	36.0	36.0	32.0	36.0
34	34.909498853879064	36.0	36.0	36.0	32.0	36.0
35	34.906269181064786	36.0	36.0	36.0	32.0	36.0
36	34.88148864034569	36.0	36.0	36.0	32.0	36.0
37	34.86859561684072	36.0	36.0	36.0	32.0	36.0
38	34.85188925815732	36.0	36.0	36.0	32.0	36.0
39	34.83412940564646	36.0	36.0	36.0	32.0	36.0
40	34.823673702606726	36.0	36.0	36.0	32.0	36.0
41	34.80403898819953	36.0	36.0	36.0	32.0	36.0
42	34.76820903817353	36.0	36.0	36.0	32.0	36.0
43	34.7677057270335	36.0	36.0	36.0	32.0	36.0
44	34.71080701644745	36.0	36.0	36.0	32.0	36.0
45	34.708845107391646	36.0	36.0	36.0	32.0	36.0
46	34.680921941017736	36.0	36.0	36.0	32.0	36.0
47	34.659924603768026	36.0	36.0	36.0	32.0	36.0
48	34.63976649041477	36.0	36.0	36.0	32.0	36.0
49	34.61954588166497	36.0	36.0	36.0	32.0	36.0
50	34.197700839000696	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	8.0
21	22.0
22	51.0
23	157.0
24	383.0
25	978.0
26	2051.0
27	3942.0
28	6730.0
29	11293.0
30	16816.0
31	24849.0
32	38444.0
33	65114.0
34	146953.0
35	578271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.957547943875056	17.98597211167387	11.840872886076713	26.215607058374367
2	16.387339136024714	19.598871008603595	36.869894790412275	27.143895064959416
3	18.871217760358366	22.796388200383234	28.3697686434994	29.962625395759
4	12.735111029767895	15.535797586338507	34.92410157287521	36.80498981101839
5	14.843326272841509	36.38894902830818	33.02747788667353	15.74024681217678
6	35.578892156260984	34.52628994548387	16.078744287523786	13.816073610731364
7	31.19312639917149	29.90973879156223	19.801108400497284	19.096026408768996
8	28.493436867373607	33.21105811402285	18.717371265063065	19.578133753540474
9	26.898465068421302	14.291469601569528	18.69873424502213	40.11133108498704
10	16.67388339698192	26.4689208161006	30.83600984748891	26.02118593942857
11	37.632830617387555	21.198996502489774	21.466499119484503	19.701673760638165
12	24.346309312037285	23.54190427937228	28.4231295462611	23.68865686232934
13	29.580633569402252	19.18374316177603	25.125381389317305	26.110241879504407
14	23.37283191193506	19.274584684610286	24.883434925552358	32.4691484779023
15	25.341436903085263	27.039414507413518	21.705544011267026	25.913604578234196
16	25.99176168480353	25.7686731982452	23.30644144289928	24.933123674051984
17	24.16317548037756	26.07107065774173	24.871605439777873	24.894148422102834
18	25.00407336066763	24.985771137393897	25.729466356272862	24.280689145665608
19	25.66819854787482	24.958875797095303	25.18162724620731	24.191298408822565
20	25.890086098664717	24.317320730080965	24.761373337871696	25.03121983338262
21	26.530746619110644	24.322873538333116	24.55232092278917	24.594058919767072
22	26.00199092477563	24.158153528869526	24.96579493028415	24.874060616070693
23	24.533599683058707	24.233175048685084	25.58419311099083	25.649032157265378
24	24.886475362726422	24.673208632001618	25.275622976560747	25.164693028711216
25	25.03336815227484	24.224720555674594	25.418831690593528	25.32307960145704
26	24.74106231132786	25.20174317711327	25.54256666648067	24.514627845078202
27	25.337031002388233	24.695221302144947	25.00267839207196	24.965069303394863
28	24.749066462512303	24.57876422626315	25.547790646545852	25.124378664678694
29	24.604269337904437	24.840859581458467	25.50498625098207	25.04988482965502
30	24.67705953710844	24.886866213647924	25.64328624215039	24.79278800709325
31	25.283824909632468	24.74435391261552	24.814215071931326	25.157606105820683
32	25.165585085903363	24.652787465195047	24.6860439811844	25.495583467717186
33	24.579327862010164	24.64662201221785	25.138103666470997	25.63594645930099
34	25.31253696763811	24.576978619592126	25.39523287659003	24.71525153617974
35	25.708597357783912	24.39117207884241	25.442657125702794	24.457573437670884
36	24.6642256180648	25.009067442802568	25.117430359249294	25.209276579883333
37	25.596479656804483	24.77520760583406	24.986914778098075	24.64139795926339
38	24.8886800749052	24.625083420194493	25.32090270136352	25.165333803536793
39	25.42128687236626	24.503495286050995	24.856929862152704	25.218287979430045
40	25.66546585958146	24.546014570387833	25.176996285979573	24.611523284051138
41	24.642711265169286	25.06590004620257	25.540983021112567	24.750405667515572
42	25.649699228820467	25.000725422139887	25.11154760443289	24.238027744606764
43	25.185484446984034	24.314756615835147	25.170976036570124	25.32878290061069
44	24.715828241399514	24.918835969978517	25.158673028096313	25.206662760525656
45	24.98404096227417	25.135261214851056	24.955247733370236	24.925450089504533
46	24.936051961965983	24.852573545823848	25.036828713003885	25.174545779206287
47	25.070224483988145	24.823587837800133	25.297889080469393	24.808298597742326
48	25.733210568901537	25.225747049468485	24.464495968304455	24.57654641332552
49	24.965012488365396	25.30261128483964	24.61725782726995	25.115118399525016
50	25.173538261001543	25.29897684966118	24.619108627139376	24.908376262197898
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	139.0
1	87.5
2	36.0
3	116.0
4	196.0
5	187.0
6	178.0
7	139.5
8	101.0
9	142.5
10	184.0
11	299.0
12	414.0
13	777.0
14	1140.0
15	1478.5
16	1817.0
17	1790.5
18	1764.0
19	1713.5
20	1663.0
21	2020.0
22	2377.0
23	2849.5
24	3322.0
25	4206.5
26	5091.0
27	6316.5
28	7542.0
29	8732.0
30	9922.0
31	11455.5
32	12989.0
33	15123.0
34	17257.0
35	20208.5
36	23160.0
37	27296.5
38	31433.0
39	34998.5
40	38564.0
41	42017.0
42	45470.0
43	47798.0
44	50126.0
45	54194.5
46	58263.0
47	61846.0
48	65429.0
49	68096.0
50	70763.0
51	69299.0
52	67835.0
53	67199.5
54	66564.0
55	66981.5
56	67399.0
57	65236.0
58	63073.0
59	57811.5
60	52550.0
61	45797.0
62	39044.0
63	33933.5
64	28823.0
65	24123.0
66	19423.0
67	16584.0
68	13745.0
69	12211.5
70	10678.0
71	8466.5
72	6255.0
73	5522.5
74	4790.0
75	3517.0
76	2244.0
77	1942.0
78	1640.0
79	1388.0
80	1136.0
81	917.5
82	699.0
83	555.0
84	411.0
85	332.5
86	254.0
87	171.0
88	88.0
89	55.5
90	23.0
91	21.5
92	20.0
93	15.0
94	10.0
95	9.5
96	9.0
97	8.0
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031136099349824677
2	0.005021951508036238
3	3.347967672024159E-4
4	0.0
5	0.0
6	1.115989224008053E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0010043903016072476
17	0.0
18	0.0
19	0.0
20	1.115989224008053E-4
21	0.0
22	0.0
23	1.115989224008053E-4
24	5.579946120040265E-4
25	2.231978448016106E-4
26	1.115989224008053E-4
27	6.695935344048318E-4
28	0.0013391870688096636
29	2.231978448016106E-4
30	3.347967672024159E-4
31	3.347967672024159E-4
32	1.115989224008053E-4
33	0.0
34	0.0013391870688096636
35	0.0
36	3.347967672024159E-4
37	0.00189718168081369
38	0.0
39	2.231978448016106E-4
40	2.231978448016106E-4
41	0.0013391870688096636
42	0.0040175612064289904
43	0.00379436336162738
44	0.004575555818433017
45	0.0024551762928177166
46	0.0029015719824209375
47	0.0012275881464088583
48	0.004575555818433017
49	0.0035711655168257695
50	0.001115989224008053
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	896066.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.37531394010766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.02867970109128	49.117853408667976
2	12.674742338595143	15.558325802825024
3	3.358718128777257	6.184271387701053
4	1.3382823459614188	3.2854999649554344
5	0.676600027254517	2.0763269542315683
6	0.408796587114124	1.5054011323064358
7	0.2787580398592513	1.1976203546783413
8	0.1981236436665033	0.972792066319173
9	0.14799405341576066	0.8174863340695236
>10	0.7648746753606551	8.58627450593574
>50	0.06898859140556426	2.9434444025363202
>100	0.05178233833644509	6.2304235417975615
>500	0.0034765527039456318	1.4066995567259999
>1k	1.8297645810240167E-4	0.11758058724989
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1047	0.11684407175364316	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.231978448016106E-4
2	0.0	0.0	0.0	0.0	2.231978448016106E-4
3	0.0	0.0	0.0	0.0	2.231978448016106E-4
4	0.0	0.0	0.0	0.0	2.231978448016106E-4
5	0.0	0.0	0.0	0.0	2.231978448016106E-4
6	0.0	0.0	0.0	0.0	2.231978448016106E-4
7	0.0	0.0	0.0	0.0	2.231978448016106E-4
8	0.0	0.0	0.0	0.0	2.231978448016106E-4
9	0.0	0.0	0.0	0.0	2.231978448016106E-4
10	0.0	0.0	0.0	0.0	2.231978448016106E-4
11	0.0	0.0	0.0	0.0	2.231978448016106E-4
12	0.0	0.0	0.0	0.0	2.231978448016106E-4
13	0.0	0.0	0.0	0.0	3.347967672024159E-4
14	0.0	0.0	0.0	0.0	3.347967672024159E-4
15	0.0	0.0	0.0	1.115989224008053E-4	4.463956896032212E-4
16	0.0	0.0	0.0	1.115989224008053E-4	4.463956896032212E-4
17	0.0	0.0	0.0	1.115989224008053E-4	4.463956896032212E-4
18	0.0	0.0	0.0	2.231978448016106E-4	4.463956896032212E-4
19	0.0	0.0	0.0	3.347967672024159E-4	4.463956896032212E-4
20	0.0	0.0	0.0	4.463956896032212E-4	4.463956896032212E-4
21	0.0	0.0	0.0	5.579946120040265E-4	4.463956896032212E-4
22	0.0	0.0	0.0	6.695935344048318E-4	4.463956896032212E-4
23	0.0	0.0	0.0	0.001115989224008053	4.463956896032212E-4
24	0.0	0.0	0.0	0.0013391870688096636	4.463956896032212E-4
25	0.0	0.0	0.0	0.001450785991210469	4.463956896032212E-4
26	0.0	0.0	0.0	0.001450785991210469	4.463956896032212E-4
27	0.0	0.0	0.0	0.0016739838360120794	4.463956896032212E-4
28	0.0	0.0	0.0	0.0039059622840281853	4.463956896032212E-4
29	0.0	0.0	0.0	0.006695935344048318	4.463956896032212E-4
30	0.0	0.0	0.0	0.010378699783274893	4.463956896032212E-4
31	0.0	0.0	0.0	0.017409431894525627	4.463956896032212E-4
32	0.0	0.0	0.0	0.026448944608990856	4.463956896032212E-4
33	0.0	0.0	0.0	0.034484067021848835	4.463956896032212E-4
34	0.0	0.0	0.0	0.04609035495153259	4.463956896032212E-4
35	0.0	0.0	0.0	0.05579946120040265	4.463956896032212E-4
36	0.0	0.0	0.0	0.06986092542290412	4.463956896032212E-4
37	0.0	0.0	0.0	0.08961393468784666	4.463956896032212E-4
38	0.0	0.0	0.0	0.11505848899523026	4.463956896032212E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTG	25	0.0023526007	35.199596	14
TCGATCG	45	4.0687E-5	29.332998	41
GTATCAA	1185	0.0	25.072798	1
GTATACG	50	0.002574246	22.008345	1
GTCTTAG	115	8.725692E-9	21.05146	1
GGTATCA	545	0.0	20.998789	1
TATACCG	55	0.004483018	19.999771	5
GTACTAG	90	9.982108E-6	19.562973	1
CAAGACG	395	0.0	19.493448	4
CGTCGTA	215	0.0	19.441639	10
CGCAAGA	400	0.0	18.701874	2
AACCGCT	60	0.0074111307	18.333124	7
GCATTCG	240	0.0	18.333124	22
ATACCGT	230	0.0	18.173706	6
GGTATAT	85	1.4261603E-4	18.124521	1
AAGACGG	405	0.0	17.925722	5
CCGTCGT	210	0.0	17.80932	9
GAGCGAA	385	0.0	17.714083	16
ACCGTCG	225	0.0	17.5998	8
CGATCGT	75	0.0012906156	17.5998	42
>>END_MODULE