FastQCFastQC Report
Thu 2 Feb 2017
SRR4062285_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062285_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046989
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG173311.6553182507170563No Hit
TCCATGTACTCTGCGTTGATACCAC150851.4407983273940796No Hit
CTGTAGGACGTGGAATATGGCAAGA142121.3574163625405806No Hit
GTCCTAAAGTGTGTATTTCTCATTT140581.3427075165068592No Hit
GTCCTACAGTGGACATTTCTAAATT134721.286737491988932No Hit
CTTTAGGACGTGAAATATGGCGAGG109991.050536347564301No Hit
GTACATGGGAAGCAGTGGTATCAAC99560.9509173448813694No Hit
CCCATGTACTCTGCGTTGATACCAC95130.9086055345376121No Hit
GTCCTACAGTGTGCATTTCTCATTT78070.7456620843198926No Hit
GAGTACATGGAAGCAGTGGTATCAA74020.7069797294909498No Hit
CATGTACTCTGCGTTGATACCACTG72540.69284395538062No Hit
CTGAAGGACCTGGAATATGGCGAGA65660.6271317081650333No Hit
GTCCTTCAGTGTGCATTTCTCATTT63530.6067876548846263No Hit
ATTTAGAAATGTCCACTGTAGGACG59750.5706841237109463No Hit
CTGTAGGACCTGGAATATGGCGAGA57540.5495759745326837No Hit
GCTTCCATGTACTCTGCGTTGATAC49720.4748856005172929No Hit
TTTCTAAATTTTCCACCTTTTTCAG43190.4125162728548247No Hit
GCGTTGATACCACTGCTTCCATGTA42770.40850476939108243No Hit
GTATCAACGCAGAGTACATGGGAAG41120.39274529149780946No Hit
CATGGAAGCAGTGGTATCAACGCAG40700.3887337880340672No Hit
GAATATGGCAAGAAAACTGAAAATC37460.35778790417091294No Hit
GTATCAACGCAGAGTACATGGAAGC36220.34594441775415025No Hit
ACTCTGCGTTGATACCACTGCTTCC36140.3451803218562946No Hit
GGAATATGGCGAGAAAACTGAAAAT34800.33238171556721224No Hit
ATTCCAGGTCCTTCAGTGTGCATTT33140.3165267256867073No Hit
CAGTGGTATCAACGCAGAGTACATG32610.31146459036341356No Hit
GAGTACATGGGAAGCAGTGGTATCA32250.30802615882306306No Hit
CCATATTCCAGGTCCTTCAGTGTGC29700.2836706020789139No Hit
GCCATATTCCACGTCCTACAGTGGA29340.2802321705385634No Hit
GTCCACTGTAGGACGTGGAATATGG29170.2786084667556202No Hit
GAAATATGGCGAGGAAAACTGAAAA27250.26027016520708435No Hit
TTCCAGGTCCTTCAGTGTGCATTTC27220.2599836292453884No Hit
GCTTCCCATGTACTCTGCGTTGATA25370.2423139116074763No Hit
ACAGTGGACATTTCTAAATTTTCCA25350.2421228876330124No Hit
GCGTTGATACCACTGCTTCCCATGT25270.2413587917351567No Hit
ACGCAGAGTACATGGAAGCAGTGGT24360.2326672008970486No Hit
TATCAACGCAGAGTACATGGGAAGC24310.2321896409608888No Hit
ATACACACTTTAGGACGTGAAATAT24220.23133003307580116No Hit
ACCTGGAATATGGCGAGAAAACTGA24000.22922876935669811No Hit
CTGTAGGACATGGAATATGGCAAGA23840.2277005775609868No Hit
CCATATTTCACGTCCTAAAGTGTGT23590.22531277788018786No Hit
GACCTGGAATATGGCGAGAAAACTG23530.22473970595679613No Hit
CCACTGTAGGACGTGGAATATGGCA23030.21996410659519822No Hit
TATCAACGCAGAGTACATGGAAGCA22890.21862693877395084No Hit
GGTATCAACGCAGAGTACATGGGAA21830.20850266812736332No Hit
ATTCCACGTCCTACAGTGGACATTT21420.20458667665085306No Hit
GTGTATTTCTCATTTTCCGTGATTT21250.20296297286790976No Hit
CCTAAAGTGTGTATTTCTCATTTTC21200.20248541293175No Hit
CACTTTAGGACGTGAAATATGGCGA21090.20143478107219848No Hit
GAAATACACACTTTAGGACGTGAAA20690.19761430158292018No Hit
ACGCAGAGTACATGGGAAGCAGTGG20290.19379382209364188No Hit
CTACAGTGGACATTTCTAAATTTTC20260.19350728613194598No Hit
GTAGGACGTGGAATATGGCAAGAAA20000.19102397446391509No Hit
GTATCAACGCAGAGTACTTTTTTTT19550.186725935038477No Hit
TCCTAAAGTGTGTATTTCTCATTTT19500.1862483751023172No Hit
GGTCCTTCAGTGTGCATTTCTCATT18930.18080419183009563No Hit
GTACTCTGCGTTGATACCACTGCTT17460.16676392970699788No Hit
AAAAAGTACTCTGCGTTGATACCAC17230.16456715400066285No Hit
AGTGTGTATTTCTCATTTTCCGTGA17080.1631344741921835No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16770.16017360258799282No Hit
ATACCACTGCTTCCATGTACTCTGC16220.15492044329023513No Hit
ATATTTCACGTCCTAAAGTGTGTAT16190.15463390732853927No Hit
GATATACACTGTTCTACAAATCCCG15510.14813909219676616No Hit
GGTATCAACGCAGAGTACATGGAAG15250.14565578052873526No Hit
CCTTCAGTGTGCATTTCTCATTTTT15030.1435545168096322No Hit
AAGCAGTGGTATCAACGCAGAGTAC14760.14097569315436934No Hit
GTCCTACAGTGGACATTTCTAAATA14220.13581804584384363No Hit
TTGTAGAACAGTGTATATCAATGAG14150.13514946193321992No Hit
GTATTTCTCATTTTCCGTGATTTTC13510.12903669475037466No Hit
ACGTGGAATATGGCAAGAAAACTGA13440.12836811083975094No Hit
TTTCTAAATATTCCACCTTTTTCAG13350.12750850295466332No Hit
ACGTCCTACAGTGGACATTTCTAAA13250.12655338308234376No Hit
TTTTTCAAGTCGTCAAGTGGATGTT13100.12512070327386438No Hit
CACTGAAGGACCTGGAATATGGCGA12980.1239745594270809No Hit
GTGGTATCAACGCAGAGTACATGGA12720.12149124775905No Hit
TTCCACGTCCTACAGTGGACATTTC12630.12063163987396239No Hit
GTGTATATCAATGAGTTACAATGAA12520.11958100801441084No Hit
CGTCCTAAAGTGTGTATTTCTCATT12350.11795730423146758No Hit
GTGAAATATGGCGAGGAAAACTGAA12230.11681116038468407No Hit
TATCAACGCAGAGTACTTTTTTTTT12220.11671564839745212No Hit
CCACTGCTTCCATGTACTCTGCGTT11920.1138502887804934No Hit
GAAGCAGTGGTATCAACGCAGAGTA11750.11222658499755012No Hit
GTGTGTATTTCTCATTTTCCGTGAT11730.1120355610230862No Hit
GTGCATTTCTCATTTTTCACGTTTT11520.11002980929121509No Hit
CTTGACGACTTGAAAAATGACGAAA11400.1088836654444316No Hit
GAGAAATACACACTTTAGGACGTGA11300.10792854557211203No Hit
ATATTCCACGTCCTACAGTGGACAT11070.105731769865777No Hit
GTGGAATATGGCAAGAAAACTGAAA10680.10200680236373066No Hit
GTGGTATCAACGCAGAGTACATGGG10600.101242706465875No Hit
GTCAAGTGGATGTTTCTCATTTTCC10490.10019207460632347No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACC250.006029384719.00076512
GACCTTG453.5344267E-516.8903777
TAAGGCA453.53878E-516.88795316
GCTCGTT402.7591834E-416.62567112
ATACCAA1400.016.28481319
GAAGAAC601.4650759E-615.833973
CGAATGA551.1271732E-515.54608114
CAGGACG508.706603E-515.2006124
CCACCCT755.8362275E-815.20061213
TATACTG456.755195E-414.779085
CACCCAT456.755195E-414.779086
TGTAAAG653.3731849E-614.6138772
AAGAACC801.2863893E-714.2505754
ATCGAGG400.005279511714.24921119
GTATTAT400.005291250514.2444431
GTCCCAA602.5761354E-514.2444421
TAGGACC20900.013.9551084
TCGTCTT759.659598E-713.93389414
GCGAATG551.9585167E-413.81873813
TAGAAAT16900.013.6603144
Produced by FastQC (version 0.11.5)