##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062285_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1046989
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.218450241597573	32.0	32.0	32.0	32.0	32.0
2	31.62187186302817	32.0	32.0	32.0	32.0	32.0
3	31.672068187917926	32.0	32.0	32.0	32.0	32.0
4	31.73027796853644	32.0	32.0	32.0	32.0	32.0
5	31.73475270513826	32.0	32.0	32.0	32.0	32.0
6	35.42231102714546	36.0	36.0	36.0	36.0	36.0
7	35.42883163051379	36.0	36.0	36.0	36.0	36.0
8	35.39794305384297	36.0	36.0	36.0	36.0	36.0
9	35.43107234173425	36.0	36.0	36.0	36.0	36.0
10	35.3574536122156	36.0	36.0	36.0	36.0	36.0
11	35.415395959269866	36.0	36.0	36.0	36.0	36.0
12	35.36794846937265	36.0	36.0	36.0	36.0	36.0
13	35.36025306856137	36.0	36.0	36.0	36.0	36.0
14	35.340785815323756	36.0	36.0	36.0	36.0	36.0
15	35.30464789983467	36.0	36.0	36.0	36.0	36.0
16	35.303146451395385	36.0	36.0	36.0	36.0	36.0
17	35.32277034429206	36.0	36.0	36.0	36.0	36.0
18	35.286301957327154	36.0	36.0	36.0	36.0	36.0
19	35.22486482666007	36.0	36.0	36.0	36.0	36.0
20	35.18726653288621	36.0	36.0	36.0	36.0	36.0
21	35.0958453240674	36.0	36.0	36.0	36.0	36.0
22	34.97242186880664	36.0	36.0	36.0	36.0	36.0
23	34.94346454451766	36.0	36.0	36.0	36.0	36.0
24	34.91532193747976	36.0	36.0	36.0	36.0	36.0
25	34.82215476953435	36.0	36.0	36.0	32.0	36.0
26	34.77580089189094	36.0	36.0	36.0	32.0	36.0
27	34.613440064795334	36.0	36.0	36.0	32.0	36.0
28	34.46281097509143	36.0	36.0	36.0	32.0	36.0
29	34.37533727670491	36.0	36.0	36.0	32.0	36.0
30	34.26511835367898	36.0	36.0	36.0	32.0	36.0
31	34.186803299748135	36.0	36.0	36.0	32.0	36.0
32	34.123375699267136	36.0	36.0	36.0	32.0	36.0
33	34.20968988212866	36.0	36.0	36.0	32.0	36.0
34	34.13395938257231	36.0	36.0	36.0	32.0	36.0
35	34.02526578598247	36.0	36.0	36.0	32.0	36.0
36	33.834345919584635	36.0	36.0	36.0	32.0	36.0
37	33.53141819064002	36.0	36.0	36.0	21.0	36.0
38	33.41822120385219	36.0	36.0	36.0	21.0	36.0
39	32.74717212883803	36.0	36.0	36.0	14.0	36.0
40	32.83026373725034	36.0	36.0	36.0	14.0	36.0
41	32.87392035637433	36.0	36.0	36.0	14.0	36.0
42	32.68625362826162	36.0	36.0	36.0	14.0	36.0
43	32.72080317940303	36.0	36.0	36.0	14.0	36.0
44	32.5857301270596	36.0	36.0	36.0	14.0	36.0
45	32.25723956985222	36.0	36.0	36.0	14.0	36.0
46	32.12422957643299	36.0	36.0	36.0	14.0	36.0
47	32.09589212494114	36.0	36.0	36.0	14.0	36.0
48	32.11867268901584	36.0	36.0	36.0	14.0	36.0
49	32.1340940544743	36.0	36.0	36.0	14.0	36.0
50	31.744482511277578	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	8.0
21	37.0
22	108.0
23	394.0
24	1138.0
25	2804.0
26	5830.0
27	10973.0
28	18889.0
29	29468.0
30	43166.0
31	61138.0
32	86461.0
33	114696.0
34	189975.0
35	481900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.289802204773764	20.2547585698207	13.530275033010458	26.92516419239508
2	9.008120948838595	20.672504732921844	49.11655309564609	21.202821222593467
3	19.79122890130126	27.302709496993266	29.342282212526648	23.563779389178823
4	10.710332200242792	19.115291564667825	38.52275429827821	31.651621936811182
5	8.639823341028416	41.49613797279628	37.3801443950223	12.48389429115301
6	30.319059012306766	36.31733023873313	19.96905399790828	13.39455675105183
7	25.742581822731665	31.12898034267791	24.05717729603654	19.07126053855389
8	22.257635944599226	45.92550638067831	20.004317141822884	11.812540532899583
9	26.45481471152037	16.099023007882604	20.968892700878424	36.4772695797186
10	17.154525978782967	28.517969147717885	35.79321272716332	18.534292146335826
11	31.248561350692317	18.71853476970627	31.05534060052207	18.97756327907934
12	28.670119743378393	22.18466478635401	34.19672986058116	14.948485609686443
13	33.80436661703227	23.706648302895257	24.714681816141333	17.77430326393114
14	18.625410582155112	27.97956807569134	24.982974988275902	28.412046353877646
15	20.1352640763179	42.583350923457644	22.352670371895027	14.928714628329429
16	16.71615817287993	25.796552181940264	40.521843280823624	16.965446364356183
17	18.46027035623106	33.12460780390243	33.64170970277625	14.773412137090267
18	20.105311718292587	22.50744278582256	43.84381085916062	13.543434636724239
19	25.180493777871593	27.19168969301492	26.88519172598757	20.742624803125917
20	24.434400809179103	27.740459050589934	33.5991755389513	14.225964601279673
21	17.759993428769004	28.04645325447856	25.688928621913526	28.50462469483891
22	22.429653033603984	36.924265679964165	24.70264730575011	15.943433980681746
23	14.077607310105456	36.48672526645457	30.78179426908974	18.653873154350237
24	24.03304758948399	29.41856300293703	31.471811647842596	15.076577759736384
25	20.745263069420222	30.503941791007712	30.39391166644062	18.356883473131447
26	13.526732926705801	32.97923754472362	33.7991147923659	19.694914736204687
27	18.50561373849673	34.20325983657836	29.877982970147617	17.413143454777288
28	16.013153986263386	32.454395152547846	27.541803448575575	23.990647412613196
29	20.037326191563213	29.4067924499468	27.87085978227025	22.68502157621974
30	17.845360647501668	28.57337976538189	32.686455684837696	20.89480390227875
31	23.497926428815667	25.433004834830648	29.792375447236164	21.276693289117524
32	16.83722005302851	33.040428507025894	31.11938673370367	19.00296470624193
33	13.97684216357574	34.50943610677858	35.39607388425284	16.117647845392835
34	16.119539553613045	33.29277238238598	35.232248057276905	15.355440006724075
35	16.41671155734354	34.943475951968885	32.81737708550623	15.82243540518134
36	15.44535371943136	31.75566482137331	35.721135294769375	17.077846164425953
37	17.30393421014929	32.10856089477254	27.79372856910895	22.793776325969226
38	19.3737470021175	37.01653025963024	25.66397545723976	17.9457472810125
39	22.98929399999809	28.48976535435628	29.25806818257794	19.26287246306769
40	25.545827129033828	28.166007474768122	30.52152410388266	15.766641292315391
41	23.08271998074458	25.73473368112797	33.41636595993421	17.766180378193237
42	21.494961998886282	34.44103451668333	31.331505153575694	12.732498330854689
43	16.66661890830107	33.98389778945629	31.60734600076222	17.742137301480415
44	15.532928984192177	33.669134151583165	32.67940207268733	18.11853479153732
45	18.23548851727471	34.03116057476666	29.62604253823436	18.10730836972427
46	17.666599807062283	27.614831370526378	34.296875746200946	20.4216930762104
47	26.399222903338575	26.86026587097674	30.693503372576586	16.0470078531081
48	15.90666176351193	34.535720596592945	28.427472312299116	21.130145327596004
49	21.198951619373645	28.073314051891785	32.163966118661094	18.56376821007347
50	15.784964435500124	39.4089234563326	25.75811555429902	19.047996553868256
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	240.0
1	172.0
2	104.0
3	317.5
4	531.0
5	485.0
6	439.0
7	413.5
8	388.0
9	477.0
10	566.0
11	734.5
12	903.0
13	1366.5
14	1830.0
15	2218.5
16	2607.0
17	2774.5
18	2942.0
19	2858.5
20	2775.0
21	3033.5
22	3292.0
23	3749.5
24	4207.0
25	5880.0
26	7553.0
27	13545.0
28	19537.0
29	29855.5
30	40174.0
31	55867.5
32	71561.0
33	97565.0
34	123569.0
35	146808.0
36	170047.0
37	159826.0
38	149605.0
39	124918.5
40	100232.0
41	79346.5
42	58461.0
43	49279.0
44	40097.0
45	47929.0
46	55761.0
47	64751.5
48	73742.0
49	64244.5
50	54747.0
51	39872.5
52	24998.0
53	17672.0
54	10346.0
55	8699.5
56	7053.0
57	6421.5
58	5790.0
59	5026.5
60	4263.0
61	3604.5
62	2946.0
63	2420.0
64	1894.0
65	1704.5
66	1515.0
67	1169.5
68	824.0
69	680.0
70	536.0
71	432.5
72	329.0
73	285.5
74	242.0
75	201.5
76	161.0
77	117.5
78	74.0
79	64.0
80	54.0
81	41.0
82	28.0
83	19.0
84	10.0
85	7.5
86	5.0
87	3.0
88	1.0
89	4.0
90	7.0
91	4.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0335247075184171
2	0.006399303144541155
3	4.775599361597878E-4
4	0.0
5	0.0
6	3.820479489278302E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.640958978556604E-4
17	0.0
18	1.910239744639151E-4
19	0.0
20	1.910239744639151E-4
21	9.551198723195755E-5
22	0.0
23	0.0
24	0.0013371678212474056
25	2.865359616958726E-4
26	2.865359616958726E-4
27	3.820479489278302E-4
28	5.730719233917452E-4
29	2.865359616958726E-4
30	0.001050631859551533
31	2.865359616958726E-4
32	4.775599361597878E-4
33	0.0
34	4.775599361597878E-4
35	9.551198723195755E-5
36	9.551198723195755E-5
37	0.0018147277574071933
38	0.0
39	5.730719233917452E-4
40	0.0
41	0.001050631859551533
42	0.004393551412670047
43	0.004966623336061793
44	0.003724967502046344
45	0.002387799680798939
46	0.0018147277574071933
47	0.0014326798084793632
48	0.004202527438206132
49	0.00391599147651026
50	0.0017192157701752357
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1046989.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.576384654478396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.45914423300239	32.55353935216554
2	12.530657513575058	10.670201885430028
3	4.232631945211163	5.406304974004308
4	1.9948429578240527	3.3973280439037716
5	1.12747935974588	2.400199745526281
6	0.7134499497523552	1.8225671695424734
7	0.4781389200904657	1.4250198606034001
8	0.3526559350820991	1.201185179419217
9	0.25586577212484685	0.9804455580512337
>10	1.5874456745453305	12.664299036632116
>50	0.14409006918716208	4.296633699766135
>100	0.10199591497491205	8.473034229345574
>500	0.010021432678324517	2.8666015044070963
>1k	0.010244131182287284	7.969082017692694
>5k	0.0011134925198138352	2.9160011466455806
>10k+	2.2269850396276704E-4	0.9575565968645937
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	10099	0.9645755590555394	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	7729	0.7382121493157999	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	7299	0.6971419948060581	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	5363	0.5122307875249883	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	5184	0.4951341418104679	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	5179	0.4946565818743081	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	4755	0.4541594992879581	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	4430	0.4231181034375719	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	3807	0.3636141353920624	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	3599	0.3437476420478152	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	3322	0.317290821584563	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	3197	0.30535182318056825	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	2662	0.254252910011471	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	2653	0.25339330212638334	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	2499	0.2386844560926619	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	2406	0.22980184128008987	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	2286	0.21834040281225492	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	2285	0.218244890825023	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	2258	0.21566606716976017	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	1986	0.18968680664266768	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	1876	0.17918048804715236	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1747	0.16685944169422984	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1723	0.16456715400066285	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	1676	0.16007809060076086	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	1596	0.15243713162220426	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	1571	0.1500493319414053	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	1537	0.14680192437551876	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT	1485	0.14183530103945696	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA	1472	0.14059364520544151	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT	1472	0.14059364520544151	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAG	1444	0.13791930956294668	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	1324	0.1264578710951118	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG	1297	0.12387904743984895	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	1295	0.12368802346538503	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATAT	1267	0.12101368782289021	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	1262	0.12053612788673043	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG	1255	0.11986754397610672	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATT	1217	0.11623808846129233	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATG	1193	0.11394580076772534	No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	1180	0.11270414493370991	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1175	0.11222658499755012	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	1141	0.10897917743166356	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCAC	1139	0.10878815345719964	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	1111	0.10611381781470484	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	1081	0.1032484581977461	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACAC	1079	0.1030574342232822	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	1072	0.10238885031265849	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1071	0.10229333832542653	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1064	0.10162475441480284	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	9.551198723195755E-5	0.0
21	0.0	0.0	0.0	9.551198723195755E-5	0.0
22	0.0	0.0	0.0	1.910239744639151E-4	0.0
23	0.0	0.0	0.0	4.775599361597877E-4	0.0
24	0.0	0.0	0.0	0.001050631859551533	0.0
25	0.0	0.0	0.0	0.0015281917957113208	0.0
26	0.0	0.0	0.0	0.0020057517318711085	0.0
27	0.0	0.0	0.0	0.0030563835914226415	0.0
28	0.0	0.0	0.0	0.004202527438206132	0.0
29	0.0	0.0	0.0	0.005921743208381368	0.0
30	0.0	0.0	0.0	0.007449935004092688	0.0
31	0.0	0.0	0.0	0.011747974429530779	0.0
32	0.0	0.0	0.0	0.01757420565068019	0.0
33	0.0	0.0	0.0	0.022827364948437855	0.0
34	0.0	0.0	0.0	0.029417692067442925	0.0
35	0.0	0.0	0.0	0.036294555148143864	0.0
36	0.0	0.0	0.0	0.045750241884107666	0.0
37	0.0	0.0	0.0	0.06303791157309198	0.0
38	0.0	0.0	0.0	0.08519669261090614	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGTTG	25	0.0023528251	35.199463	35
CGGTTAT	35	3.2169558E-4	31.429596	43
CCATTCG	35	3.2178572E-4	31.428095	9
ACTATCC	50	2.3613338E-6	30.79953	20
ACCTACT	45	4.069451E-5	29.33289	8
CCGGTCC	30	0.0057450924	29.332888	4
GGACCCG	30	0.0057450924	29.332888	6
TAGGACC	2275	0.0	29.20395	4
TCGTATA	55	4.95896E-6	28.000912	44
GTCTAGT	40	7.0164335E-4	27.510094	1
CGACGTG	40	7.032124E-4	27.499582	6
GAGACCC	40	7.032124E-4	27.499582	29
GAACGTA	40	7.032124E-4	27.499582	6
CTCGATG	40	7.032124E-4	27.499582	10
TACGGTG	50	8.341245E-5	26.399597	5
CACCTAC	50	8.341245E-5	26.399597	7
GACCTTG	85	8.234565E-9	25.88196	7
TGGTATA	755	0.0	25.643219	44
TATAGGA	355	0.0	25.411705	2
GTATAGG	70	1.1284137E-6	25.152086	1
>>END_MODULE