FastQCFastQC Report
Thu 2 Feb 2017
SRR4062284_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062284_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56948
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15022.637493854042284No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11181.9631944932218865No Hit
TATCAACGCAGAGTACTTTTTTTTT10921.9175388073330055No Hit
GTACATGGGAAGCAGTGGTATCAAC9131.6032169698672474No Hit
GTACATGGAAGCAGTGGTATCAACG8011.406546322961298No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7561.3275268666151576No Hit
GTATCAACGCAGAGTACATGGGAAG7261.2748472290510642No Hit
CCCATGTACTCTGCGTTGATACCAC6791.1923157968673175No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6601.1589520264100583No Hit
GGTATCAACGCAGAGTACTTTTTTT6311.1080283767647678No Hit
TCCATGTACTCTGCGTTGATACCAC6131.0764205942263116No Hit
TATCAACGCAGAGTACATGGGAAGC4480.7866825876237972No Hit
GTACATGGGAGTGGTATCAACGCAA4340.7620987567605535No Hit
GGTATCAACGCAGAGTACATGGGAA3860.6778113366580037No Hit
GAGTACATGGAAGCAGTGGTATCAA3760.6602514574699726No Hit
CATGTACTCTGCGTTGATACCACTG3600.6321556507691227No Hit
GCGTTGATACCACTGCTTCCCATGT2820.4951885931024794No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2710.4758727259956451No Hit
ACGCAGAGTACATGGGAAGCAGTGG2710.4758727259956451No Hit
GTACATGGGGTGGTATCAACGCAAA2670.4688487743204327No Hit
GAGTACATGGGAAGCAGTGGTATCA2520.4425089555383859No Hit
ACTCTGCGTTGATACCACTGCTTCC2400.4214371005127485No Hit
AAAAAGTACTCTGCGTTGATACCAC2390.4196811125939453No Hit
GTATCAACGCAGAGTACATGGGAGT2170.38104937838027675No Hit
GCTTCCATGTACTCTGCGTTGATAC2130.3740254267050643No Hit
CAGTGGTATCAACGCAGAGTACATG2090.3670014750298518No Hit
ATCAACGCAGAGTACTTTTTTTTTT2010.3529535716794268No Hit
CATGGAAGCAGTGGTATCAACGCAG1950.34241764416660814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1820.31958980122216757No Hit
GTATCAACGCAGAGTACATGGGGTG1810.31783381330336447No Hit
GTACATGGGTGGTATCAACGCAAAA1750.3072978857905458No Hit
GCGTTGATACCACTGCTTCCATGTA1700.29851794619653016No Hit
GTATCAACGCAGAGTACATGGAAGC1680.29500597035892395No Hit
GCTTCCCATGTACTCTGCGTTGATA1510.2651541757392709No Hit
AAAGTACTCTGCGTTGATACCACTG1400.24583830863243664No Hit
GGTATCAACGCAGAGTACATGGGAG1350.237058369038421No Hit
GTGGTATCAACGCAGAGTACATGGG1320.23179040528201167No Hit
GTATCAACGCAGAGTACATGGGACG1280.2247664536067992No Hit
TATCAACGCAGAGTACATGGGGTGG1270.22301046568799607No Hit
GTACATGGGACGCAGAGTACTTTTT1230.2159865140127836No Hit
TATCAACGCAGAGTACATGGGAGTG1180.207206574418768No Hit
GTACTCTGCGTTGATACCACTGCTT1100.19315867106834303No Hit
GTACATGGGAGAGTACTTTTTTTTT1050.18437873147432746No Hit
ATCAACGCAGAGTACATGGGAAGCA1040.18262274355552433No Hit
GGTATCAACGCAGAGTACATGGGGT1020.1791107677179181No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT960.16857484020509939No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT960.16857484020509939No Hit
GGTATCAACGCAGAGTACATGGGAC950.16681885228629628No Hit
ACGCAGAGTACATGGAAGCAGTGGT940.16506286436749315No Hit
AAGCAGTGGTATCAACGCAGAGTAC930.16330687644869002No Hit
TATCAACGCAGAGTACATGGAAGCA920.16155088852988692No Hit
GAGTGGTATCAACGCAAAAAAAAAA890.15628292477347755No Hit
ATACCACTGCTTCCCATGTACTCTG850.14925897309826508No Hit
GCAGTGGTATCAACGCAGAGTACAT830.14574699726065884No Hit
ACGCAGAGTACATGGGAGTGGTATC820.14399100934185574No Hit
CAACGCAGAGTACTTTTTTTTTTTT820.14399100934185574No Hit
GTATCAACGCAGAGTACATGGGCAG760.13345508182903704No Hit
ACGCAGAGTACATGGGGTGGTATCA750.1316990939102339No Hit
GGTATCAACGCAGAGTACATGGAAG750.1316990939102339No Hit
GAGTACATGGGGTGGTATCAACGCA750.1316990939102339No Hit
TATCAACGCAGAGTACATGGGACGC740.12994310599143077No Hit
AACGCAGAGTACTTTTTTTTTTTTT710.12467514223502142No Hit
GTGGTATCAACGCAGAGTACATGGA690.12116316639741519No Hit
GTATCAACGCAGAGTACATGGGTGG670.11765119055980894No Hit
CCCCATGTACTCTGCGTTGATACCA660.11589520264100583No Hit
GGGAAGCAGTGGTATCAACGCAGAG660.11589520264100583No Hit
TCAACGCAGAGTACTTTTTTTTTTT640.1123832268033996No Hit
ACGCAGAGTACATGGGACGCAGAGT640.1123832268033996No Hit
TATCAACGCAGAGTACATGGGTGGT640.1123832268033996No Hit
GTACATGGGCAGTGGTATCAACGCA620.10887125096579336No Hit
CTGCTTCCCATGTACTCTGCGTTGA600.10535927512818713No Hit
GTATCAACGCAGAGTACATGGGAGA590.103603287209384No Hit
GAAGCAGTGGTATCAACGCAGAGTA590.103603287209384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGGT1009.316864E-913.3006159
GACGCAG701.0552585E-412.2148519
GGGAGTG1103.531568E-812.0914687
GGAGTGG1103.531568E-812.0914688
GGGACGC752.0068113E-411.4005277
GGACGCA752.0068113E-411.4005278
TCCATGT1158.2580664E-710.7396271
ACATGGA2350.010.5111263
ATGGAAG2201.8189894E-129.9322785
CATGGAA2500.09.8804584
TGGGACG900.00107873439.500446
TGCGTTG3750.09.37376712
CTGCGTT3750.09.37376711
TGGAAGC2355.456968E-129.2983036
CTCTGCG3850.09.1302939
TCTGCGT3900.09.01323710
TGTACTC3550.08.8313945
ATGTACT3700.08.7301344
ACTCTGC4150.08.4702718
CCATGTA3750.08.3603872
Produced by FastQC (version 0.11.5)