##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062284_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56948
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.8239446512608	32.0	32.0	32.0	14.0	32.0
2	30.207434852848213	32.0	32.0	32.0	21.0	32.0
3	30.304558544637214	32.0	32.0	32.0	21.0	32.0
4	29.96505584041582	32.0	32.0	32.0	21.0	32.0
5	30.39592259605254	32.0	32.0	32.0	32.0	32.0
6	33.637546533679846	36.0	36.0	36.0	27.0	36.0
7	33.24329212615017	36.0	36.0	36.0	21.0	36.0
8	32.903613823136894	36.0	36.0	36.0	14.0	36.0
9	32.64881997611857	36.0	36.0	36.0	14.0	36.0
10	33.07106483107396	36.0	36.0	36.0	14.0	36.0
11	33.325489920629344	36.0	36.0	36.0	21.0	36.0
12	33.21930533117932	36.0	36.0	36.0	21.0	36.0
13	33.141866263960104	36.0	36.0	36.0	21.0	36.0
14	32.9618599424036	36.0	36.0	36.0	14.0	36.0
15	33.12321767226241	36.0	36.0	36.0	21.0	36.0
16	33.22308070520475	36.0	36.0	36.0	21.0	36.0
17	33.01360890637073	36.0	36.0	36.0	14.0	36.0
18	32.99227365315727	36.0	36.0	36.0	14.0	36.0
19	32.96535435836201	36.0	36.0	36.0	14.0	36.0
20	32.62448198356395	36.0	36.0	36.0	14.0	36.0
21	32.401875395097285	36.0	36.0	36.0	14.0	36.0
22	32.45497646976189	36.0	36.0	36.0	14.0	36.0
23	32.57619231579687	36.0	36.0	36.0	14.0	36.0
24	32.58214511484161	36.0	36.0	36.0	14.0	36.0
25	32.01113296340521	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	4.0
6	13.0
7	3.0
8	9.0
9	6.0
10	1.0
11	2.0
12	1.0
13	4.0
14	132.0
15	159.0
16	188.0
17	247.0
18	329.0
19	340.0
20	453.0
21	549.0
22	637.0
23	775.0
24	918.0
25	925.0
26	1077.0
27	1250.0
28	1440.0
29	1748.0
30	2186.0
31	2836.0
32	3688.0
33	5036.0
34	10106.0
35	21886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.107185502563745	17.563391163868793	12.75900821802346	17.570415115544005
2	12.144412446442368	24.92624850741027	40.001404790335044	22.92793425581232
3	19.74608414694107	35.94331670998104	26.578633139004005	17.731966004073893
4	10.585652822899288	18.23689524980244	33.082799192203005	38.094652735095266
5	10.05269629369401	43.089759353592136	29.413314596873352	17.444229755840507
6	27.35275513072814	34.52171773966826	20.9024458813607	17.223081248242902
7	24.935836585451604	36.06687058327181	21.45167528038533	17.545617550891258
8	31.700242590444045	28.124670393418416	20.94012586576662	19.234961150370918
9	31.475035161744024	14.940225035161744	20.30239099859353	33.28234880450071
10	17.150242667229374	31.571358233101215	28.100161778152916	23.178237321516495
11	29.89342618972249	27.37680700643664	20.247265308993704	22.482501494847174
12	26.050257618654054	30.23018622399634	28.387289640740676	15.33226651660893
13	30.87568137858273	23.13873747142606	26.653771760154736	19.33180938983647
14	25.996835443037973	22.89029535864979	29.437412095639942	21.675457102672294
15	25.691477202792285	24.859770357475693	32.34864869617204	17.100103743559984
16	18.540461665582534	29.786747771663645	31.22132170672105	20.45146885603277
17	18.68560779211646	28.279826998136365	31.551742325679527	21.482822884067655
18	20.749309938641677	24.525747639726436	36.17679635717928	18.54814606445261
19	20.001757623692768	29.30661745320327	35.91528253800861	14.77634238509535
20	22.70402334206317	23.222540558592446	37.06607139718419	17.007364702160196
21	23.678799275909945	25.077769380832706	32.011107400833055	19.232323942424294
22	22.050551922941715	26.995008085495325	31.38754130633481	19.56689868522815
23	17.441043123747935	29.148068744947807	32.74698625803957	20.66390187326468
24	17.779808773903262	32.44340551181102	32.874015748031496	16.90276996625422
25	20.45690185396714	27.092522625428344	31.837272647394784	20.613302873209737
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	262.0
1	262.0
2	343.5
3	425.0
4	425.0
5	425.0
6	951.5
7	1478.0
8	1478.0
9	1478.0
10	1333.5
11	1189.0
12	1189.0
13	1189.0
14	851.0
15	513.0
16	513.0
17	513.0
18	717.5
19	922.0
20	922.0
21	922.0
22	1307.0
23	1692.0
24	1692.0
25	1692.0
26	2393.0
27	3094.0
28	3094.0
29	3094.0
30	3456.5
31	3819.0
32	3819.0
33	3819.0
34	3539.0
35	3259.0
36	3259.0
37	3259.0
38	3403.5
39	3548.0
40	3548.0
41	3548.0
42	4536.5
43	5525.0
44	5525.0
45	5525.0
46	9323.5
47	13122.0
48	13122.0
49	13122.0
50	10806.0
51	8490.0
52	8490.0
53	8490.0
54	5992.5
55	3495.0
56	3495.0
57	3495.0
58	2859.0
59	2223.0
60	2223.0
61	2223.0
62	1935.5
63	1648.0
64	1648.0
65	1648.0
66	1349.0
67	1050.0
68	1050.0
69	1050.0
70	812.0
71	574.0
72	574.0
73	574.0
74	440.0
75	306.0
76	306.0
77	306.0
78	237.0
79	168.0
80	168.0
81	168.0
82	120.0
83	72.0
84	72.0
85	72.0
86	51.0
87	30.0
88	30.0
89	30.0
90	28.5
91	27.0
92	27.0
93	27.0
94	14.0
95	1.0
96	1.0
97	1.0
98	8.5
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.005267963756409356
5	0.031607782538456135
6	0.06321556507691227
7	0.10887125096579336
8	0.10887125096579336
9	0.11940717847861207
10	0.1404790335042495
11	0.1510149610170682
12	0.1422350214230526
13	0.13696705766664327
14	0.11940717847861207
15	0.13521106974784014
16	0.11765119055980894
17	0.12291915431621832
18	0.12116316639741519
19	0.09306735969656528
20	0.09657933553417151
21	0.08604340802135282
22	0.09833532345297465
23	0.07375149258973099
24	0.09131137177776216
25	0.0755074805085341
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	56948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.113998735688696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.81927573207417	39.57469972606589
2	8.091847225924374	7.462948654913254
3	2.0981683865808614	2.902648029781555
4	1.0205247324930504	1.882419048956943
5	0.6130764251170938	1.4135702746365104
6	0.33509767335592705	0.9271616211280467
7	0.23989947069799322	0.7743906721921754
8	0.2360915425916759	0.8709700077263469
9	0.16374090857164617	0.6795673245768069
>10	1.0852595103004454	9.952939523776076
>50	0.12566162750847265	4.228418908477909
>100	0.12946955561478998	12.664184870408091
>500	0.030463424850538823	10.147854182763224
>1k	0.011423784318952057	6.518227154597176
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1502	2.637493854042284	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1118	1.9631944932218865	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1092	1.9175388073330055	No Hit
GTACATGGGAAGCAGTGGTATCAAC	913	1.6032169698672474	No Hit
GTACATGGAAGCAGTGGTATCAACG	801	1.406546322961298	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	756	1.3275268666151576	No Hit
GTATCAACGCAGAGTACATGGGAAG	726	1.2748472290510642	No Hit
CCCATGTACTCTGCGTTGATACCAC	679	1.1923157968673175	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	660	1.1589520264100583	No Hit
GGTATCAACGCAGAGTACTTTTTTT	631	1.1080283767647678	No Hit
TCCATGTACTCTGCGTTGATACCAC	613	1.0764205942263116	No Hit
TATCAACGCAGAGTACATGGGAAGC	448	0.7866825876237972	No Hit
GTACATGGGAGTGGTATCAACGCAA	434	0.7620987567605535	No Hit
GGTATCAACGCAGAGTACATGGGAA	386	0.6778113366580037	No Hit
GAGTACATGGAAGCAGTGGTATCAA	376	0.6602514574699726	No Hit
CATGTACTCTGCGTTGATACCACTG	360	0.6321556507691227	No Hit
GCGTTGATACCACTGCTTCCCATGT	282	0.4951885931024794	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	271	0.4758727259956451	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	271	0.4758727259956451	No Hit
GTACATGGGGTGGTATCAACGCAAA	267	0.4688487743204327	No Hit
GAGTACATGGGAAGCAGTGGTATCA	252	0.4425089555383859	No Hit
ACTCTGCGTTGATACCACTGCTTCC	240	0.4214371005127485	No Hit
AAAAAGTACTCTGCGTTGATACCAC	239	0.4196811125939453	No Hit
GTATCAACGCAGAGTACATGGGAGT	217	0.38104937838027675	No Hit
GCTTCCATGTACTCTGCGTTGATAC	213	0.3740254267050643	No Hit
CAGTGGTATCAACGCAGAGTACATG	209	0.3670014750298518	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	201	0.3529535716794268	No Hit
CATGGAAGCAGTGGTATCAACGCAG	195	0.34241764416660814	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	182	0.31958980122216757	No Hit
GTATCAACGCAGAGTACATGGGGTG	181	0.31783381330336447	No Hit
GTACATGGGTGGTATCAACGCAAAA	175	0.3072978857905458	No Hit
GCGTTGATACCACTGCTTCCATGTA	170	0.29851794619653016	No Hit
GTATCAACGCAGAGTACATGGAAGC	168	0.29500597035892395	No Hit
GCTTCCCATGTACTCTGCGTTGATA	151	0.2651541757392709	No Hit
AAAGTACTCTGCGTTGATACCACTG	140	0.24583830863243664	No Hit
GGTATCAACGCAGAGTACATGGGAG	135	0.237058369038421	No Hit
GTGGTATCAACGCAGAGTACATGGG	132	0.23179040528201167	No Hit
GTATCAACGCAGAGTACATGGGACG	128	0.2247664536067992	No Hit
TATCAACGCAGAGTACATGGGGTGG	127	0.22301046568799607	No Hit
GTACATGGGACGCAGAGTACTTTTT	123	0.2159865140127836	No Hit
TATCAACGCAGAGTACATGGGAGTG	118	0.207206574418768	No Hit
GTACTCTGCGTTGATACCACTGCTT	110	0.19315867106834303	No Hit
GTACATGGGAGAGTACTTTTTTTTT	105	0.18437873147432746	No Hit
ATCAACGCAGAGTACATGGGAAGCA	104	0.18262274355552433	No Hit
GGTATCAACGCAGAGTACATGGGGT	102	0.1791107677179181	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	96	0.16857484020509939	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	96	0.16857484020509939	No Hit
GGTATCAACGCAGAGTACATGGGAC	95	0.16681885228629628	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	94	0.16506286436749315	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	93	0.16330687644869002	No Hit
TATCAACGCAGAGTACATGGAAGCA	92	0.16155088852988692	No Hit
GAGTGGTATCAACGCAAAAAAAAAA	89	0.15628292477347755	No Hit
ATACCACTGCTTCCCATGTACTCTG	85	0.14925897309826508	No Hit
GCAGTGGTATCAACGCAGAGTACAT	83	0.14574699726065884	No Hit
ACGCAGAGTACATGGGAGTGGTATC	82	0.14399100934185574	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	82	0.14399100934185574	No Hit
GTATCAACGCAGAGTACATGGGCAG	76	0.13345508182903704	No Hit
ACGCAGAGTACATGGGGTGGTATCA	75	0.1316990939102339	No Hit
GGTATCAACGCAGAGTACATGGAAG	75	0.1316990939102339	No Hit
GAGTACATGGGGTGGTATCAACGCA	75	0.1316990939102339	No Hit
TATCAACGCAGAGTACATGGGACGC	74	0.12994310599143077	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	71	0.12467514223502142	No Hit
GTGGTATCAACGCAGAGTACATGGA	69	0.12116316639741519	No Hit
GTATCAACGCAGAGTACATGGGTGG	67	0.11765119055980894	No Hit
CCCCATGTACTCTGCGTTGATACCA	66	0.11589520264100583	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	66	0.11589520264100583	No Hit
TCAACGCAGAGTACTTTTTTTTTTT	64	0.1123832268033996	No Hit
ACGCAGAGTACATGGGACGCAGAGT	64	0.1123832268033996	No Hit
TATCAACGCAGAGTACATGGGTGGT	64	0.1123832268033996	No Hit
GTACATGGGCAGTGGTATCAACGCA	62	0.10887125096579336	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	60	0.10535927512818713	No Hit
GTATCAACGCAGAGTACATGGGAGA	59	0.103603287209384	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	59	0.103603287209384	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTGGT	100	9.316864E-9	13.300615	9
GACGCAG	70	1.0552585E-4	12.214851	9
GGGAGTG	110	3.531568E-8	12.091468	7
GGAGTGG	110	3.531568E-8	12.091468	8
GGGACGC	75	2.0068113E-4	11.400527	7
GGACGCA	75	2.0068113E-4	11.400527	8
TCCATGT	115	8.2580664E-7	10.739627	1
ACATGGA	235	0.0	10.511126	3
ATGGAAG	220	1.8189894E-12	9.932278	5
CATGGAA	250	0.0	9.880458	4
TGGGACG	90	0.0010787343	9.50044	6
TGCGTTG	375	0.0	9.373767	12
CTGCGTT	375	0.0	9.373767	11
TGGAAGC	235	5.456968E-12	9.298303	6
CTCTGCG	385	0.0	9.130293	9
TCTGCGT	390	0.0	9.013237	10
TGTACTC	355	0.0	8.831394	5
ATGTACT	370	0.0	8.730134	4
ACTCTGC	415	0.0	8.470271	8
CCATGTA	375	0.0	8.360387	2
>>END_MODULE