##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062284_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56948
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.90521177214301	32.0	32.0	32.0	21.0	32.0
2	30.953202921963896	32.0	32.0	32.0	32.0	32.0
3	31.352444335182973	32.0	32.0	32.0	32.0	32.0
4	31.616351759499896	32.0	32.0	32.0	32.0	32.0
5	31.61594788227857	32.0	32.0	32.0	32.0	32.0
6	35.126729648100024	36.0	36.0	36.0	36.0	36.0
7	35.08407670155229	36.0	36.0	36.0	36.0	36.0
8	35.09310247945494	36.0	36.0	36.0	36.0	36.0
9	34.98540774039475	36.0	36.0	36.0	36.0	36.0
10	35.00063215565077	36.0	36.0	36.0	36.0	36.0
11	35.05761396361593	36.0	36.0	36.0	36.0	36.0
12	34.992730210016155	36.0	36.0	36.0	36.0	36.0
13	34.9176968462457	36.0	36.0	36.0	36.0	36.0
14	34.834761536840624	36.0	36.0	36.0	32.0	36.0
15	34.87722132471728	36.0	36.0	36.0	32.0	36.0
16	34.87005689400857	36.0	36.0	36.0	32.0	36.0
17	34.85922244854955	36.0	36.0	36.0	32.0	36.0
18	34.79121303645431	36.0	36.0	36.0	32.0	36.0
19	34.696512607993256	36.0	36.0	36.0	32.0	36.0
20	34.52182692983072	36.0	36.0	36.0	32.0	36.0
21	34.4652314392077	36.0	36.0	36.0	32.0	36.0
22	34.42422912130365	36.0	36.0	36.0	32.0	36.0
23	34.47929690243731	36.0	36.0	36.0	32.0	36.0
24	34.41381611294514	36.0	36.0	36.0	32.0	36.0
25	34.32067851373183	36.0	36.0	36.0	32.0	36.0
26	34.202465407038	36.0	36.0	36.0	32.0	36.0
27	34.158091592329846	36.0	36.0	36.0	32.0	36.0
28	34.059686029360115	36.0	36.0	36.0	32.0	36.0
29	33.90203343400997	36.0	36.0	36.0	32.0	36.0
30	33.902103673526724	36.0	36.0	36.0	32.0	36.0
31	33.732229402261716	36.0	36.0	36.0	32.0	36.0
32	33.69925194914659	36.0	36.0	36.0	27.0	36.0
33	33.47311582496312	36.0	36.0	36.0	21.0	36.0
34	33.256163517595	36.0	36.0	36.0	21.0	36.0
35	33.03972044672333	36.0	36.0	36.0	14.0	36.0
36	32.85722062232212	36.0	36.0	36.0	14.0	36.0
37	32.68302662077685	36.0	36.0	36.0	14.0	36.0
38	32.54000140479034	36.0	36.0	36.0	14.0	36.0
39	32.20998103533048	36.0	36.0	36.0	14.0	36.0
40	31.968023459998594	36.0	36.0	36.0	14.0	36.0
41	31.875254618248228	36.0	32.0	36.0	14.0	36.0
42	31.55225820046358	36.0	32.0	36.0	14.0	36.0
43	31.44328159022266	36.0	32.0	36.0	14.0	36.0
44	31.370970007726346	36.0	32.0	36.0	14.0	36.0
45	30.896045515206854	36.0	32.0	36.0	14.0	36.0
46	30.84336587764276	36.0	32.0	36.0	14.0	36.0
47	30.46547727751633	36.0	27.0	36.0	14.0	36.0
48	30.310247945494137	36.0	21.0	36.0	14.0	36.0
49	30.175809510430568	36.0	21.0	36.0	14.0	36.0
50	29.577122989393832	36.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	8.0
20	11.0
21	33.0
22	64.0
23	133.0
24	241.0
25	478.0
26	921.0
27	1460.0
28	2354.0
29	2818.0
30	3536.0
31	3953.0
32	4875.0
33	5476.0
34	7828.0
35	22758.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.52093872962339	20.120151770657674	13.503021360314785	17.85588813940416
2	11.714606820496611	25.380184736416954	39.56379728163523	23.3414111614512
3	19.897099107958137	35.18297394113928	27.272248366931233	17.647678583971345
4	10.362084708857203	17.333356746505586	33.87125096579335	38.43330757884386
5	9.775584743976962	41.955819343962915	30.120460771229894	18.14813514083023
6	27.391655545409847	35.139074243169205	19.918170962983776	17.551099248437172
7	23.026269579265293	36.07325981597246	22.422209735197022	18.478260869565215
8	31.390040036524546	26.827983423474045	21.630259183816815	20.15171735618459
9	30.187188312144414	13.573786612348107	21.851513661586008	34.38751141392147
10	17.108590292898786	30.099388916204255	29.382945845332586	23.409074945564374
11	30.145044602093137	25.72171103462808	21.58284751000913	22.55039685326965
12	25.68483528833322	29.3917257849266	29.663903912341084	15.2595350143991
13	31.305752616422	22.36426213387652	28.28194142024303	18.048043829458454
14	26.527709489358713	20.852356535787035	31.79040528201166	20.829528692842594
15	24.947320362435907	23.769052468919014	34.64212966214792	16.641497506497156
16	18.585376132612208	27.66207768490553	32.96867317552855	20.783873006953712
17	18.376413570274636	25.935941560722064	33.99768209594718	21.68996277305612
18	19.91114701130856	21.02444335182974	40.893446653087025	18.170962983774672
19	20.053382032731616	25.077263468427336	40.524689190138375	14.344665308702675
20	22.50649715529957	19.869003301257287	41.14455292547588	16.47994661796727
21	22.434501650628643	22.654000140479035	36.47889302521599	18.432605183676337
22	21.133314602795533	24.00435485003863	35.89063707241694	18.971693474748893
23	16.876799887616773	25.12994310599143	37.86085551731404	20.132401489077758
24	17.670506426915782	28.023811196178972	37.49385404228419	16.811828334621058
25	20.61354217882981	22.253634894991922	36.575472360750155	20.55735056542811
26	17.514223502142308	23.598721640795112	39.348177284540284	19.5388775725223
27	19.142024302872795	25.55138020650418	37.71686450797219	17.58973098265084
28	18.162183044180654	23.593453677038703	40.438645782117014	17.805717496663622
29	17.501931586710683	24.202781484863383	41.109433167099816	17.185853761326122
30	18.959734489964355	24.914394085728837	40.834460112034	15.291411312272816
31	18.402753389056684	24.564514996136825	38.56325068483529	18.4694809299712
32	14.597527569010325	26.722624148345858	40.489569431762305	18.190278850881505
33	16.18142867177074	26.701552293320223	39.395588958347965	17.721430076561074
34	14.783662288403457	26.671700498700567	40.24197513521107	18.302662077684907
35	16.595841820608275	27.39341153332865	39.82053803469832	16.190208611364753
36	17.738989955749105	27.93249982440121	38.08737795883964	16.241132261010044
37	17.738989955749105	28.98609257568308	37.725644447566204	15.549273021001616
38	17.930392638898645	28.613823136896816	36.382313689681816	17.073470534522723
39	17.632184311728448	27.667831492440335	37.14330869053681	17.556675505294397
40	16.880311863454377	30.28552363559739	36.187399030694664	16.646765470253563
41	15.71960384912552	30.34347123691789	35.931024794549415	18.00590011940718
42	18.798840986917202	31.14057423830011	35.68004214593029	14.380542628852403
43	18.027288531441517	29.31848912146382	34.711222715858604	17.942999631236063
44	17.426198127952514	30.32506190400927	35.83934812004988	16.40939184798834
45	18.53302193267424	29.968216060547526	34.37933517129963	17.119426835478603
46	15.686515531985881	31.350202820166118	35.371485767467995	17.59179588038
47	18.04133668147576	31.17460094473809	33.98598697034084	16.79807540344531
48	17.2177638857183	34.67083428451016	31.385323195251726	16.726078634519816
49	17.00412678900694	33.74308543331285	33.305821406620424	15.946966371059796
50	16.793102237207176	35.80058300846416	29.524461770800407	17.881852983528255
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	114.0
1	111.0
2	108.0
3	387.0
4	666.0
5	613.0
6	560.0
7	446.5
8	333.0
9	416.0
10	499.0
11	716.5
12	934.0
13	1443.0
14	1952.0
15	2318.5
16	2685.0
17	2447.5
18	2210.0
19	1926.0
20	1642.0
21	1619.0
22	1596.0
23	1444.0
24	1292.0
25	1082.0
26	872.0
27	842.0
28	812.0
29	894.5
30	977.0
31	1212.5
32	1448.0
33	1631.5
34	1815.0
35	1772.5
36	1730.0
37	1777.0
38	1824.0
39	1849.5
40	1875.0
41	2170.0
42	2465.0
43	2775.0
44	3085.0
45	3565.0
46	4045.0
47	4617.5
48	5190.0
49	4755.5
50	4321.0
51	3490.5
52	2660.0
53	2169.5
54	1679.0
55	1672.5
56	1666.0
57	1627.5
58	1589.0
59	1423.0
60	1257.0
61	1100.0
62	943.0
63	804.0
64	665.0
65	546.0
66	427.0
67	372.0
68	317.0
69	297.0
70	277.0
71	215.0
72	153.0
73	132.0
74	111.0
75	79.5
76	48.0
77	41.0
78	34.0
79	32.0
80	30.0
81	24.0
82	18.0
83	16.0
84	14.0
85	10.5
86	7.0
87	4.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03511975837606238
2	0.0035119758376062375
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0017559879188031187
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0017559879188031187
40	0.0
41	0.0
42	0.005267963756409356
43	0.0017559879188031187
44	0.008779939594015594
45	0.0017559879188031187
46	0.0017559879188031187
47	0.0017559879188031187
48	0.0017559879188031187
49	0.005267963756409356
50	0.0035119758376062375
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	56948.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.66067289457049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.20715166461159	54.644588045234244
2	7.653289989911445	9.591206012502635
3	1.9504539849792624	3.666502774460912
4	0.9191794641856293	2.303856149469692
5	0.45678735567761464	1.4311301538245418
6	0.3418899226544109	1.285383156563883
7	0.21017823114000672	0.9218936573716373
8	0.14852595000560476	0.7445388775725222
9	0.11769980943840377	0.6637634333075788
>10	0.8238986660688263	9.721149118494065
>50	0.1064903037776034	4.892182341785489
>100	0.05604752830400179	6.48661937205872
>500	0.00840712924560027	3.6471869073540777
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	961	1.6875043899697972	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	573	1.006181077474187	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	543	0.9535014399100934	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	360	0.6321556507691227	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	331	0.5812320011238323	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	315	0.5531361944229823	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	236	0.414413148837536	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	221	0.3880733300554892	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	216	0.3792933904614736	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	213	0.3740254267050643	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	210	0.3687574629486549	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	170	0.29851794619653016	No Hit
ATACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	167	0.2932499824401208	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	160	0.280958067008499	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	130	0.2282784294444054	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	130	0.2282784294444054	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	130	0.2282784294444054	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	129	0.22652244152560227	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	125	0.2194984898503898	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	123	0.2159865140127836	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	122	0.21423052609398047	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	103	0.18086675563672122	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT	103	0.18086675563672122	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	100	0.17559879188031186	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	99	0.17384280396150875	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	97	0.17033082812390252	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	94	0.16506286436749315	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	94	0.16506286436749315	No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	93	0.16330687644869002	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92	0.16155088852988692	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA	90	0.15803891269228068	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	88	0.15452693685467445	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	85	0.14925897309826508	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	83	0.14574699726065884	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	82	0.14399100934185574	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	81	0.1422350214230526	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	79	0.13872304558544638	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	78	0.13696705766664327	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	77	0.13521106974784014	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	75	0.1316990939102339	No Hit
ATACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	74	0.12994310599143077	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	72	0.12643113015382454	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	72	0.12643113015382454	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	71	0.12467514223502142	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	70	0.12291915431621832	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68	0.11940717847861207	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66	0.11589520264100583	No Hit
GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTTTTTTTTTTTTTT	65	0.1141392147222027	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	64	0.1123832268033996	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	64	0.1123832268033996	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	63	0.11062723888459647	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	61	0.10711526304699023	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	60	0.10535927512818713	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	58	0.10184729929058088	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0017559879188031185	0.0
19	0.0	0.0	0.0	0.003511975837606237	0.0
20	0.0	0.0	0.0	0.003511975837606237	0.0
21	0.0	0.0	0.0	0.008779939594015592	0.0
22	0.0	0.0	0.0	0.010535927512818712	0.0
23	0.0	0.0	0.0	0.021071855025637423	0.0
24	0.0	0.0	0.0	0.03336377045725925	0.0
25	0.0	0.0	0.0	0.036875746294865494	0.0
26	0.0	0.0	0.0	0.04565568588888108	0.0
27	0.0	0.0	0.0	0.0667275409145185	0.0
28	0.0	0.0	0.0	0.09833532345297465	0.0
29	0.0	0.0	0.0	0.11765119055980895	0.0
30	0.0	0.0	0.0	0.19140268314953993	0.0
31	0.0	0.0	0.0	0.2897380066025146	0.0
32	0.0	0.0	0.0	0.3775374025426705	0.0
33	0.0	0.0	0.0	0.4706047622392358	0.0
34	0.0	0.0	0.0	0.5829879890426354	0.0
35	0.0	0.0	0.0	0.7445388775725224	0.0
36	0.0	0.0	0.0	0.9078457540212124	0.0
37	0.0	0.0	0.0	1.0922244854955399	0.0
38	0.0	0.0	0.0	1.3398187820467795	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACATG	55	1.8189894E-12	44.0	1
CTGGAAT	30	1.2856125E-4	36.666664	10
TGTAGGA	30	1.2856125E-4	36.666664	2
GTAGGAC	30	1.2856125E-4	36.666664	3
CCTGGAA	30	1.2856125E-4	36.666664	9
GGACCTG	40	1.789929E-5	33.0	6
AGGACCT	40	1.789929E-5	33.0	5
AAAATAA	40	1.789929E-5	33.0	42
CTGTAGG	35	3.1797765E-4	31.428572	1
ATATCAA	50	2.3096818E-6	30.8	1
GGAATAT	30	0.005699712	29.333332	12
ACCTGGA	40	6.9495593E-4	27.5	8
ATGGCGA	45	0.0013819259	24.444445	17
AAAAGTA	45	0.0013819259	24.444445	2
TGGCGAG	45	0.0013819259	24.444445	18
TATGGCG	45	0.0013819259	24.444445	16
ATATGGC	55	1.5677551E-4	24.0	15
AAAAAGT	50	0.0025506192	22.0	1
AATATGG	65	4.8532014E-4	20.307693	14
TAGGACC	55	0.0044322154	20.0	4
>>END_MODULE