FastQCFastQC Report
Thu 2 Feb 2017
SRR4062283_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062283_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347678
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20020.5758201554311748No Hit
TATCAACGCAGAGTACTTTTTTTTT14400.414176335574871No Hit
GGTATCAACGCAGAGTACTTTTTTT10360.2979768636496989No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5550.15963046266948153No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT5170.1487008093695891No Hit
ACCATACTCCCCCCGGAACCCAAAG4760.1369082887039157No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG3930.11303562491730855No Hit
TCGTAGTTCCGACCATAAACGATGC3620.10411932880423841No Hit
GAATAGGACCGCGGTTCTATTTTGT3600.10354408389371775No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3550.10210597161741611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTGT250.006021796719.0005766
GGTATCA2700.016.8796751
GCACGCC350.002165197616.2862115
GGCCGCG400.005262088514.2524847
AAATCGC400.00526712214.25043214
ATATCTG400.00526712214.2504325
TCAATAC400.005272159414.2483793
CGACCAT655.4198506E-513.1561410
CGACTGG655.4271855E-513.15424415
CTGGCGA655.4271855E-513.15424418
CGCATCG604.0767717E-412.66705113
CGACCCG751.4703086E-512.667055
GACCCGG751.4703086E-512.667056
CTTTCGA1001.4266516E-712.35215411
CGATGCC853.910818E-612.2962639
CTCCCGA853.942452E-612.2874111
GACCATA701.0844334E-412.21641511
GCGCGCG550.003056204212.09301711
CAGGACT1209.915311E-911.8736514
TCGCCAG657.9938764E-411.69266217