##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062283_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347678
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.68199023234142	32.0	21.0	32.0	14.0	32.0
2	25.66456606400175	32.0	14.0	32.0	14.0	32.0
3	26.003517622627832	32.0	21.0	32.0	14.0	32.0
4	25.75725240020939	32.0	14.0	32.0	14.0	32.0
5	26.079216976627798	32.0	21.0	32.0	14.0	32.0
6	29.30684714016993	32.0	21.0	36.0	14.0	36.0
7	29.079130689891223	32.0	21.0	36.0	14.0	36.0
8	28.88353879164054	32.0	21.0	36.0	14.0	36.0
9	29.023308923774298	32.0	21.0	36.0	14.0	36.0
10	27.894436806470356	32.0	21.0	36.0	14.0	36.0
11	29.77064410172631	36.0	21.0	36.0	14.0	36.0
12	28.58245272924948	32.0	21.0	36.0	14.0	36.0
13	29.093224190198978	32.0	21.0	36.0	14.0	36.0
14	28.509494417248142	32.0	21.0	36.0	14.0	36.0
15	28.45744338151968	32.0	21.0	36.0	14.0	36.0
16	28.57680957667727	32.0	21.0	36.0	14.0	36.0
17	28.315099028411346	32.0	21.0	36.0	14.0	36.0
18	28.38779272775384	32.0	21.0	36.0	14.0	36.0
19	28.335902760600327	32.0	21.0	36.0	14.0	36.0
20	28.338661059946272	32.0	21.0	36.0	14.0	36.0
21	28.309760755641715	32.0	21.0	36.0	14.0	36.0
22	28.103408901339744	32.0	21.0	36.0	14.0	36.0
23	28.01364480927755	32.0	21.0	36.0	14.0	36.0
24	27.91308337024488	32.0	21.0	36.0	14.0	36.0
25	27.033030562762097	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	19.0
5	39.0
6	55.0
7	34.0
8	30.0
9	30.0
10	20.0
11	16.0
12	14.0
13	13.0
14	427.0
15	995.0
16	2025.0
17	4033.0
18	7143.0
19	10549.0
20	13705.0
21	15483.0
22	16767.0
23	17228.0
24	17464.0
25	17754.0
26	18044.0
27	18763.0
28	19395.0
29	20128.0
30	20907.0
31	22999.0
32	25184.0
33	26360.0
34	30713.0
35	21337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.54262277164503	22.80155776321769	12.407457475019989	25.248361990117292
2	18.837024019420323	19.327996962698137	36.860361772564765	24.974617245316775
3	18.841859421648767	23.612653087051815	28.43723215158854	29.10825533971088
4	12.710484682870703	16.61527398245362	35.18337408312958	35.49086725154609
5	14.854889009079505	36.27832311073775	32.99462594506266	15.872161935120083
6	33.47702100353081	35.91951955754173	16.17694877601701	14.426510662910452
7	28.78144124538708	31.088044122811564	20.810664548034797	19.31985008376656
8	27.572528225411798	32.6015740596807	19.31936600054119	20.506531714366318
9	25.936534713605912	14.479044019218644	19.777413773746225	39.80700749342921
10	16.381482153294233	26.625091054621258	30.439451915961985	26.553974876122528
11	35.84451482867838	21.73221998272387	22.203570400230348	20.219694788367406
12	23.82187722243177	24.127367114776092	28.426971791852257	23.623783870939878
13	28.32645704792862	19.81982500741372	25.665579321047193	26.18813862361047
14	23.148539991536182	19.585675997017514	25.868189002219594	31.397595009226713
15	24.66931940549474	27.014171614158943	22.75692888855618	25.559580091790142
16	24.75126096870034	26.51805661116101	24.065501278242245	24.665181141896404
17	22.84759250515357	26.610850713438438	25.8784102818053	24.663146499602686
18	23.61606513108841	25.090611784282057	26.898529195851	24.394793888778533
19	24.013333064676303	25.43846266770675	26.641950219771047	23.9062540478459
20	24.767935525653016	24.067928329855366	26.09570410880046	25.068432035691156
21	25.34699634437696	24.19763391957629	26.014795198756513	24.44057453729023
22	24.522642286135397	24.37496581563183	26.36298473434068	24.739407163892096
23	23.297480723345835	24.27289813234477	26.98012036575994	25.44950077854945
24	23.454693557997025	25.034324152402114	26.68504963689796	24.8259326527029
25	23.51844016337527	24.41015367522401	26.774432173069563	25.29697398833116
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	31.0
2	101.0
3	171.0
4	171.0
5	171.0
6	433.5
7	696.0
8	696.0
9	696.0
10	837.5
11	979.0
12	979.0
13	979.0
14	1267.5
15	1556.0
16	1556.0
17	1556.0
18	2549.5
19	3543.0
20	3543.0
21	3543.0
22	5263.5
23	6984.0
24	6984.0
25	6984.0
26	9732.0
27	12480.0
28	12480.0
29	12480.0
30	14806.0
31	17132.0
32	17132.0
33	17132.0
34	20494.0
35	23856.0
36	23856.0
37	23856.0
38	26318.5
39	28781.0
40	28781.0
41	28781.0
42	32208.5
43	35636.0
44	35636.0
45	35636.0
46	38983.5
47	42331.0
48	42331.0
49	42331.0
50	43273.5
51	44216.0
52	44216.0
53	44216.0
54	41051.5
55	37887.0
56	37887.0
57	37887.0
58	35257.5
59	32628.0
60	32628.0
61	32628.0
62	28587.5
63	24547.0
64	24547.0
65	24547.0
66	20285.5
67	16024.0
68	16024.0
69	16024.0
70	12319.0
71	8614.0
72	8614.0
73	8614.0
74	6516.0
75	4418.0
76	4418.0
77	4418.0
78	3733.5
79	3049.0
80	3049.0
81	3049.0
82	2107.0
83	1165.0
84	1165.0
85	1165.0
86	854.0
87	543.0
88	543.0
89	543.0
90	343.5
91	144.0
92	144.0
93	144.0
94	90.5
95	37.0
96	37.0
97	37.0
98	133.5
99	230.0
100	230.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.8762245526032707E-4
3	0.0
4	0.008053428747289159
5	0.02358504133134682
6	0.04745770511795397
7	0.08168477729393289
8	0.08513624675705682
9	0.08830009376492041
10	0.10325646143845742
11	0.10872128808840364
12	0.10555744108054005
13	0.10038023688585417
14	0.09117631831752368
15	0.10469457371475907
16	0.09376492041486663
17	0.09951736952007316
18	0.09117631831752368
19	0.07794568537554865
20	0.07276848118086275
21	0.07708281800976766
22	0.08542386921231714
23	0.06816652189669752
24	0.0733437260913834
25	0.07391897100190406
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	347678.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.0415002410703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.07132122726686	55.98207860352517
2	8.431742381621625	10.968263482937711
3	2.2414411130666094	4.373600780876002
4	1.00935131504606	2.6259889520357174
5	0.527072603018181	1.7140796418134283
6	0.3484958863918908	1.3600017167262102
7	0.226222915926133	1.0299714488521643
8	0.17848629921087797	0.928721333852165
9	0.11591326423567987	0.6785255343355393
>10	0.7171181179275669	8.912947926262692
>50	0.07483031364253752	3.397449346371797
>100	0.05579064804114383	6.4300119993561315
>500	8.855658419229179E-4	0.30872812573609415
>1k	0.0013283487628843768	1.2896311073192441
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2002	0.5758201554311748	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1440	0.414176335574871	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1036	0.2979768636496989	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	555	0.15963046266948153	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	517	0.1487008093695891	No Hit
ACCATACTCCCCCCGGAACCCAAAG	476	0.1369082887039157	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	393	0.11303562491730855	No Hit
TCGTAGTTCCGACCATAAACGATGC	362	0.10411932880423841	No Hit
GAATAGGACCGCGGTTCTATTTTGT	360	0.10354408389371775	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	355	0.10210597161741611	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	8.628673657809813E-4
13	0.0	2.8762245526032707E-4	0.0	0.0	8.628673657809813E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGTGT	25	0.0060217967	19.000576	6
GGTATCA	270	0.0	16.879675	1
GCACGCC	35	0.0021651976	16.28621	15
GGCCGCG	40	0.0052620885	14.252484	7
AAATCGC	40	0.005267122	14.250432	14
ATATCTG	40	0.005267122	14.250432	5
TCAATAC	40	0.0052721594	14.248379	3
CGACCAT	65	5.4198506E-5	13.15614	10
CGACTGG	65	5.4271855E-5	13.154244	15
CTGGCGA	65	5.4271855E-5	13.154244	18
CGCATCG	60	4.0767717E-4	12.667051	13
CGACCCG	75	1.4703086E-5	12.66705	5
GACCCGG	75	1.4703086E-5	12.66705	6
CTTTCGA	100	1.4266516E-7	12.352154	11
CGATGCC	85	3.910818E-6	12.296263	9
CTCCCGA	85	3.942452E-6	12.287411	1
GACCATA	70	1.0844334E-4	12.216415	11
GCGCGCG	55	0.0030562042	12.093017	11
CAGGACT	120	9.915311E-9	11.873651	4
TCGCCAG	65	7.9938764E-4	11.692662	17
>>END_MODULE