FastQCFastQC Report
Thu 2 Feb 2017
SRR4062283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347678
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG5010.14409885008542386No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG4820.13863402343547765No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC4790.13777115606969667No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT4710.13547017642761405No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA4170.1199385638435564No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG3930.11303562491730855No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT3880.1115975126410069No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA3650.10498219617001939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA4450.030.1598661
CTACACG300.005741107729.3316084
ACCGTCG1200.027.4983838
CGGTCCA1053.6379788E-1227.23649210
CGAATGC1006.002665E-1126.40224643
CGCCGGT1200.025.6651577
CGCAATA1300.025.38677236
TCTTGCG1251.8189894E-1224.638552
CGTCGTA1251.8189894E-1224.6385510
GTATTAC450.001395521724.4465241
ATTAGCA450.001396691924.4430072
TACGAAT1102.0008883E-1024.0020441
TACCGTC1202.3646862E-1123.8319327
GCGCAAT1301.8189894E-1223.6943235
CCGGTCC1301.8189894E-1223.6909149
ATACGAA1254.0017767E-1122.88194540
GTCGTAG1407.2759576E-1221.99870511
ATACCGT1306.730261E-1121.9987056
GGTATCA1551.8189894E-1221.2921331
CTAGCGG1351.1277734E-1021.18698529
Produced by FastQC (version 0.11.5)