##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062283_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347678
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.076087644314565	32.0	32.0	32.0	21.0	32.0
2	28.60841065583672	32.0	32.0	32.0	14.0	32.0
3	30.233739264491856	32.0	32.0	32.0	21.0	32.0
4	30.95658914282756	32.0	32.0	32.0	32.0	32.0
5	30.738536807045598	32.0	32.0	32.0	32.0	32.0
6	34.0651321049937	36.0	36.0	36.0	32.0	36.0
7	34.25194864213439	36.0	36.0	36.0	32.0	36.0
8	33.93046151899171	36.0	36.0	36.0	32.0	36.0
9	34.46220065692969	36.0	36.0	36.0	32.0	36.0
10	33.86242730342443	36.0	36.0	36.0	32.0	36.0
11	34.571025489101984	36.0	36.0	36.0	32.0	36.0
12	34.16388727500733	36.0	36.0	36.0	32.0	36.0
13	34.40291016400232	36.0	36.0	36.0	32.0	36.0
14	34.28731757545775	36.0	36.0	36.0	32.0	36.0
15	34.147228182398656	36.0	36.0	36.0	32.0	36.0
16	34.19634259285891	36.0	36.0	36.0	32.0	36.0
17	34.18497287720247	36.0	36.0	36.0	32.0	36.0
18	34.163622662348494	36.0	36.0	36.0	32.0	36.0
19	34.1617473639402	36.0	36.0	36.0	32.0	36.0
20	34.2247798250105	36.0	36.0	36.0	32.0	36.0
21	34.256032880999086	36.0	36.0	36.0	32.0	36.0
22	34.21792290567709	36.0	36.0	36.0	32.0	36.0
23	34.05606912142845	36.0	36.0	36.0	32.0	36.0
24	34.1184429270762	36.0	36.0	36.0	32.0	36.0
25	34.04743181909698	36.0	36.0	36.0	32.0	36.0
26	33.928059871490284	36.0	36.0	36.0	32.0	36.0
27	33.93392449335305	36.0	36.0	36.0	32.0	36.0
28	33.890568284447106	36.0	36.0	36.0	32.0	36.0
29	33.79311892038035	36.0	36.0	36.0	32.0	36.0
30	33.771334395618936	36.0	36.0	36.0	32.0	36.0
31	33.840398299576044	36.0	36.0	36.0	32.0	36.0
32	33.74680307640978	36.0	36.0	36.0	32.0	36.0
33	33.66436185205851	36.0	36.0	36.0	27.0	36.0
34	33.63060648070916	36.0	36.0	36.0	27.0	36.0
35	33.61296659552804	36.0	36.0	36.0	27.0	36.0
36	33.5342443295233	36.0	36.0	36.0	27.0	36.0
37	33.555617554173686	36.0	36.0	36.0	27.0	36.0
38	33.52989547799976	36.0	36.0	36.0	27.0	36.0
39	33.530424703317436	36.0	36.0	36.0	27.0	36.0
40	33.510446447575056	36.0	36.0	36.0	27.0	36.0
41	33.46091210833012	36.0	36.0	36.0	21.0	36.0
42	33.36291913782293	36.0	36.0	36.0	21.0	36.0
43	33.36896208560795	36.0	36.0	36.0	21.0	36.0
44	33.243230805515445	36.0	36.0	36.0	21.0	36.0
45	33.187682280731025	36.0	36.0	36.0	21.0	36.0
46	33.13411547466334	36.0	36.0	36.0	21.0	36.0
47	33.13550469112224	36.0	36.0	36.0	21.0	36.0
48	33.03390493502609	36.0	36.0	36.0	14.0	36.0
49	33.016702235976965	36.0	36.0	36.0	14.0	36.0
50	32.318070168374184	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	3.0
21	10.0
22	66.0
23	184.0
24	416.0
25	894.0
26	1807.0
27	3399.0
28	6055.0
29	10301.0
30	16874.0
31	27042.0
32	42936.0
33	68608.0
34	98415.0
35	70666.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.26366659379351	20.834148362079876	13.715495518300752	25.186689525825862
2	18.817844507722842	20.66097161101044	34.95872522794604	25.56245865332068
3	21.675290571421176	22.899990508431674	28.306445350138205	27.118273570008945
4	15.235649077594788	17.388503155218334	35.313709811952435	32.06213795523444
5	19.382877259993442	33.35385040180857	30.449151226134525	16.81412111206346
6	34.97546580456629	36.14206248310218	14.941411305863472	13.941060406468054
7	29.769211741899117	30.463532348897544	19.445578955240194	20.32167695396315
8	28.495331887551124	32.456755963851606	17.72962338715708	21.318288761440183
9	26.11698180500348	13.982478040025542	17.933547707936654	41.96699244703432
10	16.749406059629887	25.860710197366526	29.454552775844316	27.935330967159267
11	37.737504242431214	20.71543209521454	21.00938224449059	20.537681417863656
12	24.330558735381587	23.325605876702006	27.247625676631827	25.09620971128458
13	29.189652494549556	18.76678996082582	24.646080568802166	27.397476975822453
14	23.868924694688765	18.74924499105494	24.20774394698543	33.17408636727087
15	25.72207617393105	26.068085987609223	21.4733172648255	26.736520573634227
16	25.92837605018451	25.798657949763715	22.725690799218814	25.54727520083296
17	24.118580985854727	26.270572196112496	24.072273770557814	25.538573047474962
18	25.2011919074546	24.792480398529673	25.11605566069754	24.890272033318187
19	25.50262024056742	25.23484373472006	24.924499105494164	24.338036919218357
20	26.223689735905058	23.827219438676018	24.23305472304834	25.71603610237058
21	26.796921289238895	24.167476803248984	24.151657568209664	24.88394433930246
22	25.747962194904478	24.24427199880349	24.61472972117879	25.393036085113234
23	24.62227872421817	24.080396459932636	25.336734958021385	25.96058985782781
24	24.524627885237653	24.8441791903358	24.899115553318474	25.732077371108076
25	24.906954135723282	24.11397902657056	25.217011142493917	25.76205569521224
26	24.854894471321167	24.72690247873032	24.952398483654417	25.465804566294096
27	25.32379191030756	24.44021318637701	24.973466752186656	25.262528151128777
28	24.6360095951955	24.46142075622566	25.566191317153425	25.336378331425415
29	24.888401845396288	24.89012759005511	25.072768899780257	25.148701664768346
30	25.04278445550848	24.587183934329097	25.397715669609088	24.972315940553337
31	25.499027830508865	24.538938551985183	24.752930889678897	25.20910272782706
32	25.585241474126846	24.696485531110774	24.21701751913414	25.50125547562824
33	24.570435863068703	24.68433435535179	25.183359315228458	25.56187046635105
34	25.321636585891998	24.596534119508163	25.195369238512978	24.88646005608686
35	26.153509856821543	24.407929175846615	24.794206133261238	24.644354834070604
36	24.323943418910602	24.926512462680986	24.957288065393843	25.792256053014572
37	25.727549370962453	24.62134068121286	24.815488072159553	24.835621875665133
38	25.087581037626773	24.18847324248299	25.231392265256936	25.49255345463331
39	25.45890162736785	24.6386023849654	24.380605042596887	25.521890945069863
40	25.743935480530837	25.098223068471405	24.70993275386996	24.4479086971278
41	24.880348370321794	24.846983973584603	25.12511648776447	25.147551168329134
42	25.84195978381861	25.322503171101413	24.543605880271866	24.29193116480811
43	25.27986469850547	24.400577562502157	25.157046377578606	25.162511361413763
44	25.14388768563022	24.82778060615474	24.462200899136242	25.566130809078803
45	25.27683979820176	25.419214551562668	24.49305959030586	24.810886059929704
46	24.829149313145724	24.96289606295589	24.83864101797096	25.369313605927424
47	25.056446393902355	24.807938448263464	25.243977852879844	24.89163730495434
48	25.9415646051095	25.451726657193973	23.90110048149661	24.705608256199916
49	24.82116713789761	25.239953864429303	24.60429544022941	25.334583557443676
50	25.866983441241864	24.94628542501388	24.385410344837506	24.801320788906754
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	7.0
2	7.0
3	11.5
4	16.0
5	22.5
6	29.0
7	32.5
8	36.0
9	52.0
10	68.0
11	103.5
12	139.0
13	224.5
14	310.0
15	441.5
16	573.0
17	704.5
18	836.0
19	937.5
20	1039.0
21	1276.5
22	1514.0
23	1879.5
24	2245.0
25	2780.0
26	3315.0
27	3783.5
28	4252.0
29	4775.5
30	5299.0
31	5657.5
32	6016.0
33	6759.5
34	7503.0
35	8328.0
36	9153.0
37	10240.0
38	11327.0
39	12818.5
40	14310.0
41	15289.0
42	16268.0
43	16726.5
44	17185.0
45	18422.5
46	19660.0
47	20839.5
48	22019.0
49	22991.5
50	23964.0
51	23368.5
52	22773.0
53	23138.0
54	23503.0
55	24393.0
56	25283.0
57	24817.0
58	24351.0
59	22351.0
60	20351.0
61	18711.5
62	17072.0
63	14882.5
64	12693.0
65	11072.0
66	9451.0
67	8361.5
68	7272.0
69	6850.5
70	6429.0
71	4989.5
72	3550.0
73	3136.0
74	2722.0
75	2155.5
76	1589.0
77	1391.5
78	1194.0
79	1059.0
80	924.0
81	746.5
82	569.0
83	484.5
84	400.0
85	294.0
86	188.0
87	141.5
88	95.0
89	71.5
90	48.0
91	36.5
92	25.0
93	26.0
94	27.0
95	21.5
96	16.0
97	21.5
98	27.0
99	31.5
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012080143120933737
2	0.0023009796420826165
3	2.8762245526032707E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.8762245526032707E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.8762245526032707E-4
24	8.628673657809813E-4
25	0.0
26	0.0
27	2.8762245526032707E-4
28	0.0011504898210413083
29	5.752449105206541E-4
30	0.0014381122763016355
31	5.752449105206541E-4
32	2.8762245526032707E-4
33	0.0
34	8.628673657809813E-4
35	0.0
36	0.0
37	0.0011504898210413083
38	0.0
39	0.0
40	0.0
41	5.752449105206541E-4
42	0.0020133571868222898
43	0.002876224552603271
44	0.0031638470078635982
45	0.0011504898210413083
46	0.0017257347315619625
47	8.628673657809813E-4
48	0.003451469463123925
49	0.0020133571868222898
50	0.0020133571868222898
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	347678.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.19127522718553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.01255851561575	62.657262767339716
2	7.3732963710703645	10.49828742568957
3	1.9553533201811926	4.17612289150231
4	0.8408372741935468	2.3944111123355705
5	0.4491255736836594	1.5986911163840494
6	0.27339307644006877	1.1677921050031144
7	0.19097810333087648	0.9517182301616013
8	0.12978330507395835	0.7391551193131162
9	0.09845014020762864	0.6307911924908666
>10	0.5858882374859065	7.7285284834153885
>50	0.0559030467040048	2.767697734654387
>100	0.03402794147174261	4.5450574448160905
>500	4.050945413302691E-4	0.1444843768942171
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	501	0.14409885008542386	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	482	0.13863402343547765	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	479	0.13777115606969667	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	471	0.13547017642761405	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	417	0.1199385638435564	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	393	0.11303562491730855	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	388	0.1115975126410069	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	365	0.10498219617001939	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.752449105206541E-4
7	0.0	0.0	0.0	0.0	5.752449105206541E-4
8	0.0	0.0	0.0	0.0	5.752449105206541E-4
9	0.0	0.0	0.0	0.0	5.752449105206541E-4
10	0.0	0.0	0.0	0.0	5.752449105206541E-4
11	0.0	0.0	0.0	0.0	5.752449105206541E-4
12	0.0	0.0	0.0	0.0	0.0011504898210413083
13	0.0	0.0	0.0	0.0	0.0011504898210413083
14	0.0	0.0	0.0	0.0	0.0011504898210413083
15	0.0	0.0	0.0	0.0	0.0011504898210413083
16	0.0	0.0	0.0	0.0	0.0011504898210413083
17	0.0	0.0	0.0	0.0	0.0011504898210413083
18	0.0	0.0	0.0	0.0	0.0011504898210413083
19	0.0	0.0	0.0	0.0	0.0011504898210413083
20	0.0	0.0	0.0	0.0	0.0011504898210413083
21	0.0	0.0	0.0	0.0	0.0011504898210413083
22	0.0	0.0	0.0	0.0	0.0014381122763016355
23	0.0	0.0	0.0	0.0	0.0017257347315619625
24	0.0	0.0	0.0	0.0	0.0017257347315619625
25	0.0	0.0	0.0	0.0	0.0017257347315619625
26	0.0	0.0	0.0	2.8762245526032707E-4	0.0020133571868222898
27	0.0	0.0	0.0	2.8762245526032707E-4	0.0020133571868222898
28	0.0	0.0	0.0	5.752449105206541E-4	0.0020133571868222898
29	0.0	0.0	0.0	8.628673657809813E-4	0.0020133571868222898
30	0.0	0.0	0.0	0.0014381122763016355	0.0020133571868222898
31	0.0	0.0	0.0	0.0017257347315619625	0.0023009796420826165
32	0.0	0.0	0.0	0.003163847007863598	0.0025886020973429438
33	0.0	0.0	0.0	0.004314336828904906	0.0025886020973429438
34	0.0	0.0	0.0	0.0051772041946858876	0.0025886020973429438
35	0.0	0.0	0.0	0.006615316470987523	0.0025886020973429438
36	0.0	0.0	0.0	0.008053428747289159	0.0025886020973429438
37	0.0	0.0	0.0	0.010642030844632102	0.0025886020973429438
38	0.0	0.0	0.0	0.013518255397235373	0.0025886020973429438
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	445	0.0	30.159866	1
CTACACG	30	0.0057411077	29.331608	4
ACCGTCG	120	0.0	27.498383	8
CGGTCCA	105	3.6379788E-12	27.236492	10
CGAATGC	100	6.002665E-11	26.402246	43
CGCCGGT	120	0.0	25.665157	7
CGCAATA	130	0.0	25.386772	36
TCTTGCG	125	1.8189894E-12	24.63855	2
CGTCGTA	125	1.8189894E-12	24.63855	10
GTATTAC	45	0.0013955217	24.446524	1
ATTAGCA	45	0.0013966919	24.443007	2
TACGAAT	110	2.0008883E-10	24.00204	41
TACCGTC	120	2.3646862E-11	23.831932	7
GCGCAAT	130	1.8189894E-12	23.69432	35
CCGGTCC	130	1.8189894E-12	23.690914	9
ATACGAA	125	4.0017767E-11	22.881945	40
GTCGTAG	140	7.2759576E-12	21.998705	11
ATACCGT	130	6.730261E-11	21.998705	6
GGTATCA	155	1.8189894E-12	21.292133	1
CTAGCGG	135	1.1277734E-10	21.186985	29
>>END_MODULE